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- 2016
孕期维生素D缺乏对子鼠Th1/Th2细胞免疫功能的影响
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Abstract:
目的·探讨孕期维生素D缺乏及补充对子鼠Th1/Th2免疫调节功能的影响。方法·48只8周龄雌性SD大鼠分为3组，对照组（C组）、维生素D缺乏组（VDD组）及维生素D补充组（VDS组）各16只。C组给予正常饲料，VDD组及VDS组给予维生素D缺乏饲料。喂养4周后按雌雄比2∶1合笼。妊娠成功后C组及VDS组给予正常饲料，VDD组继续予维生素D缺乏饲料。妊娠第10天(孕中期)用LC-MS/MS法检测维生素D水平。至子鼠4周龄时检测维生素D水平，采用流式细胞术检测Th1/Th2比例，ELISA法检测血浆中IFN-γ、IL-4表达水平。结果·C组、VDD组及VDS组孕鼠孕中期25(OH)D水平分别为（23.23±2.08）、（5.52±2.36）及（11.35±1.84）ng/mL；子鼠25(OH)D水平分别为(7.15±1.30)、(0.93±0.22)及(6.82±1.37)ng/mL；VDD组孕鼠及子鼠维生素D水平均明显低于C组和VDS组(P<0.05)。C组、VDD组及VDS组子鼠Th1/Th2细胞比例分别为0.90±0.35、0.24±0.08及0.94±0.26；与C组相比，VDD组Th1/Th2比例显著向Th2极化，而VDS组Th2极化有明显改善。C组、VDD组及VDS组IFN-γ水平分别为(18.72±5.66)、（8.27±3.86）及（19.31±5.68）pg/mL，IL-4水平分别为(3.49±0.52)、(4.14±0.55)及（3.52±0.52）pg/mL；与C组和VDS组相比，VDD组IFN-γ水平显著降低，IL-4水平显著升高（P<0.05）。结论·大鼠孕期维生素D缺乏可导致子代Th1/Th2向Th2极化，IFN-γ/IL-4向IL-4漂移。补充维生素D后子代Th2极化和IL-4漂移得到明显改善。
： Objective·To investigate the effects of maternal vitamin D deficiency and supplementation on the immunomodulatory function of Th1/Th2 in offspring rats. Methods·Forty-eight female SD (8-week-old) rats were randomly assigned to three groups, i.e control group (C group, n=16), vitamin D deficiency group (VDD group, n=16), and vitamin D supplementation group (VDS group, n=16). C group was fed a normal diet and VDD and VDS groups were fed a vitamin D deficiency diet. After four weeks, females and males were housed in a 2∶1 ratio. After gestation, C group and VDS group were fed a normal diet and VDD group was still fed a vitamin D deficiency diet. The vitamin D level in female rats was measured using LC-MS/MS at gestational day 10 (second trimester). The vitamin D level, the ratio of Th1/Th2, and expressions of serum IFN-γ and IL-4 in offspring were measured using LC-MS/MS, flow cytometry, and ELISA 4 weeks after birth, respectively. Results·For C group, VDD group, and VDS group, the 25(OH)D levels in female rats were (23.23±2.08), (5.52±2.36), and (11.35±1.84) ng/mL, respectively, while the 25(OH)D levels in offspring were (7.15±1.30), (0.93±0.22), and (6.82±1.37) ng/mL, respectively. The 25(OH)D levels in female rats and their offspring of VDD group were significantly lower than those of C group and VDS group (P<0.05). The Th1/Th2 ratios in offspring of C group, VDD group, and VDS group were 0.90±0.35, 0.24±0.08, and 0.94±0.26, respectively. The Th1/Th2 ratio in VDD group was significantly polarized toward Th2 and polarization of Th2 in VDS group was remarkably improved compared with C group. The IFN-γ levels in C group, VDD group, and VDS group were (18.72±5.66), (8.27±3.86), and (19.31±5.68) pg/ml, and the IL-4 levels were (3.49±0.52), (4.14±0.55), and (3.52±0.52) pg/ml. The
