维生素 E 拮抗双酚 A 对卵巢颗粒细胞性激素分泌的抑制作用
Vitamin E could reverse effects of bisphenol A on steroidogenesis in rat ovary granulosa cells

目的 · 研究维生素E 能否对双酚A（BPA）的雌性性激素干扰效应起拮抗作用，探索拮抗作用的适宜浓度。方法 · 提取大鼠 卵巢原代颗粒细胞。不同浓度 BPA（0、0.01、0.1、1、10、100 μmol/L）孵育 48 h 后，收集并用 ELISA 法测定细胞培养基中的雌二醇 （E2）及孕酮（P4）浓度。采用健康女性卵泡液中的平均浓度（简称“人体平均浓度”，5 μmol/L）或高浓度（25 μmol/L）维生素E(α生育酚 ) 单独孵育或与 BPA（10、100 μmol/L）共同孵育颗粒细胞48 h 后，测定培养液中性激素浓度。结果 · 10 μmol/L 及 100 μmol/L BPA 可使大鼠原代卵巢颗粒细胞分泌性激素水平呈浓度依赖性降低（P<0.05）。 25 μmol/L 维生素 E 单独孵育时可分别使 E2 和 P4 浓度 显著升高（44.89±15.18）% 和（43.33±8.82）%（P<0.05）；维生素 E（5 μmol/L 或 25 μmol/L）和 BPA 共同孵育时，性激素浓度恢复 正常，与阴性对照组相比，差异无统计学意义（P>0.05）。 结论 · 人体平均浓度及高浓度维生素 E 均可拮抗 BPA 导致的性激素降低效 应，而高浓度维生素 E 的改善效果相对显著。
： Objective · To investigate whether vitamin E could reverse the disruptive effects of bisphenol A (BPA) on steroidogenesis and to explore the optimal vitamin E concentration. Methods · Rat primary granulosa cells were extracted and exposed to BPA (0, 0.01, 0.1,1,10,100 μmol/L). After 48 h of incubation, culture medium was collected and estradiol (E2) and progesterone (P4) were measured using ELISA kits. Then, granulosa cells were incubated with 5 μmol/L(average concentration in follicular fluid) or 25 μmol/L (high concentration in follicular fluid) vitamin E (α-tocopherol) or vitamin E (5 μmol/ L or 25 μmol/L) plus BPA (10 μmol/L or 100 μmol/L) for 48 h, E2 and P4 were measured. Results · BPA at 10 μmol/L and 100 μmol/L suppressed E2 and P4 production in a dose-dependent manner (P<0.05). Vitamin E at 25 μmol/L significantly increased E2 and P4 levels by (44.89±15.18) % and (43.33±8.82) % (P<0.05), respectively. Coincubation of the granulosa cells with BPA and vitamin E (5 μmol/L or 25 μmol/L) restored the productions of E2 and P4, which were not significantly different from the control group (P>0.05). Conclusion · Vitamin E (5 μmol/L/ 25 μmol/L) could reverse BPA-induced reduction in steroid hormone production in rat ovary granulosa cells, and the antagonistic effect of 25 μmol/L vitamin E was more obvious than that of 5 μmol/L vitamin E