基质辅助激光解析电离飞行时间质谱检测β-内酰胺酶的研究进展
Research progresses of applying MALDI-TOF mass spectrometry in the detection of β-lactamase

基质辅助激光解析电离飞行时间质谱（MALDI-TOF MS）是一种检测微生物的新型工具，不仅可用于微生物的快速鉴定，而且还可以检测细菌的耐药机制。产 β-内酰胺酶是革兰阴性杆菌耐β-内酰胺类抗菌药物的主要耐药机制，目前利用MALDI-TOF MS快速检测细菌β-内酰胺酶的技术已被广泛报道，方法主要有检测 β-内酰胺酶水解β-内酰胺类抗菌药物的活性，直接检测β-内酰胺酶分子、肽段或其相关蛋白以及检测β-内酰胺酶基因的单核苷酸多态性等。该文结合最新的研究成果对MALDI-TOF MS在β-内酰胺酶检测中的应用现状进行综述。
： Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is an emerging tool for detecting microorganisms. It can be used not only for rapid identification of microorganisms, but also for the research of resistance mechanisms. Producing β-lactamase is the main mechanism for resistance of β-lactam antibiotics in Gram-negative bacilli. Currently using MALDI-TOF MS for rapid detection of bacterial β-lactamase has been widely reported, including detection of β-lactam hydrolysis activity of β-lactamase, direct detection of β-lactamase molecule, peptide and relevant proteins, and detection of the single nucleotide polymorphisms (SNPs) of β-lactamase gene. This paper reviews current application of MALDI-TOF MS to the detection of β-lactamase based on the latest research findings