佩兰精油镇静催眠作用的研究
Hypnotic effects of Eupatorium fortunei Turcz. essential oil

为了探索中草药佩兰(Eupatorium fortunei Turcz.)中挥发性小分子物质镇静的可行性,比较了定量嗅吸佩兰精油、薰衣草(Lavandula anguistifolia)精油和腹腔注射地西泮对ICR小鼠的镇静效果。结果表明,与空白对照组相比,2种精油都不能显著缩短ICR小鼠的睡眠潜伏期,但浓度为2 g/L的佩兰精油使ICR小鼠的睡眠持续期延长124.75% (P＜0.01)、自主活动量减少47.92%(P＜0.05),同时降低了小鼠体温(P＜0.05),薰衣草精油中0.2 g/L的浓度镇静催眠效果最明显,表现为使ICR小鼠的睡眠持续期延长63.91% (P＜0.05)、自主活动量减少20.81% (P＜0.05)、也同时降低了小鼠体温(P＜0.05)。通过GC-MS分析得知,佩兰精油的主要成分有β-倍半水芹烯、β-石竹烯、惕格酸百里酚酯、异丁酸百里香、石竹素、2-羟基-5-甲基苯乙酮、芳樟醇。
Quantitative inhalation of Eupatorium fortunei Turcz.essential oil (EEO),Lavandula anguistifolia essential oil (LEO) and intraperitoneal injection of diazepam were investigated to evaluate their hypnotic effects on ICR mice.The results showed that,compared with the blank control,none of the two essential oils significantly shortened the sleep latency.However,the EEO (2 g/L) increased sleep duration by 124.75% (P＜0.01),reduced locomotion by 47.92% (P＜0.05) and lowed the body temperature (P＜0.05).Similarly,the LEO (0.2 g/L) increased sleep duration by 63.91% (P＜0.05),reduced locomotion by 20.81% (P＜0.05) and lowed the body temperature (P＜0.05).Furthermore,GC-MS analysis indicated that the main components of EEO were β-sesquilpene,β-caryophyllene,thymic acid thymol,isobutyric acid thyme,carnation,2-hydroxy- acetophenone and linalool.