基于RAPD的风信子遗传多样性分析
Genetic Diversity Analysis of Hyacinth orientalis by RAPD Markers

利用RAPD分子标记法,对20个风信子品种的遗传多样性以及亲缘关系进行研究。结果表明,筛选出的12条随机引物共扩增出93条指纹谱带,其中多态性条带67条,占扩增条带总数的72.0%;使用NTSYS-pc2.10对扩增出的条带进行统计分析,得到20个风信子品种的遗传相似系数及亲缘关系树状图,以遗传相似系数0.83为分界线将20个风信子品种分为2个大类,发现各品种间亲缘关系相对较近;相同色系的品种在聚类中基本聚为一类。风信子在引进栽培过程中,在环境的驯化作用下产生了一些芽变品种,这些品种提高了风信子种群遗传多样性的丰富程度。本研究通过RAPD技术对风信子种质资源进行研究,为风信子进一步创新种质资源提供了基础。
Random amplified polymorphic DNA (RAPD) markers were used to study the genetic relationship and genetic diversity of 20 cultivars of Hyacinth orientalis.Twelve primers out of sixty RAPD markers were selected to analyze 20 cultivars of Hyacinth orientalis which generated 93 DNA fragments,including 67 polymorphism fragments accounting for 72.0% of the total.Based on those polymorphism fragments,we assessed genetic similarity coefficient and dendrogram by using NTSYS-pc (Version 2.10e).The result indicated that the cultivars can be divided into two major categories by fixing the value of 0.83 as the threshold of similarity coefficient.The genetic relationships among the cultivars were relatively close,and the cultivars with the same shade of color were basically clustered into the same category.In the process of introduction and cultivation,mutation cultivars occurred due to the influence of natural environment,which flourished the genetic diversity of Hyacinth orientalis.This research lays foundation for the further innovation of germplasm resources of Hyacinth.