基于核酸适配体的纳米金淬灭罗丹明B荧光法检测氨苄青霉素
Fluorescence Assay for Ampicillin Detection Based on Rhodamine B Quenched by Gold Nanoparticles

研究利用核酸适配体与氨苄青霉素的特异性结合效应以及纳米金对罗丹明B的荧光淬灭反应,通过改变反应体系荧光信号的强弱,从而实现氨苄青霉素的定量检测,建立了氨苄青霉素快速检测的新方法。最佳反应体系条件为适配体终浓度20 nmol/L,盐溶液NaCl终浓度0.12 mol/L,最优反应为温度30 ℃。在10 mmol/L的3-吗啉丙磺酸(MOPS)缓冲溶液(pH 6.0)的条件下,该方法可检测氨苄青霉素的浓度范围是0.494~2 000 nmol/L,其最低检测限为0.494 nmol/L,远低于国际相关检测标准。该方法具有检测时效性强、成本低廉、操作简便、反应灵敏等优点,有着良好的推广应用潜能。
A new detection method has been developed to detect ampicillin.Based on the specific binding of aptamer and ampicillin,this assay can realize the quantitative detection of ampicillin by changing the strength of the fluorescent signal in the detection system with the help of quenching reaction between gold nanoparticles and Rhodamine B.The optimized condition of this detection system was:aptamer concentration 20 nmol/L,NaCl concentration 0.12 mol/L and reaction temperature 30 ℃.The detection limit of this method is 0.494 nmol/L,much lower than the relevant maximum residue limit,with a detection range of 0.494 to 2 000 nmol/L.Because of its time-efficiency,low cost,ease of operation and strong sensitivity,this assay has a favorable application potential.