茄子抗逆相关基因SmDREB3的克隆和表达分析
Cloning and Expression Analysis of smDREB3 Related to Stress Tolerance in Eggplant(Solanum melongena L.)

DREB (dehydration responsive element binding protein)基因在植物非生物胁迫的应激反应中具有非常重要的作用,本研究运用同源克隆的方法从‘YZ-14’紫茄品种中分离克隆得到了一个DREB基因,因其与番茄和马铃薯中DREB3基因的相似度很高,所以命名为SmDREB3。SmDREB3基因的cDNA全长为887 bp,开放阅读框(ORF)长度为777 bp,可以编码一个含有259个氨基酸的蛋白,与同科作物马铃薯中DREB基因序列的相似性高达90%,与番茄中DREB基因序列的相似性也能达到88%。成熟蛋白等电点为6.39,分子量为28.665 kDa。RT-qPCR实验结果表明,SmDREB 基因在紫茄的各个组织中都会表达,但表达水平具有组织特异性,其在果皮中的表达量最高,其次为叶和果肉,而在根、花和茎中的表达量相对较低。本研究为深入研究茄子的抗逆机制以及培育抗逆茄子新品种方面提供了理论基础。
DREB has an important role in stress signal transduction pathways in plants.A cDNA fragment,named SmDREB3,was isolated from ‘YZ-14’ eggplant species using homology-based cloning method.The full length of cDNA of SmDREB is 887 bp,and the ORF is 777 bp,encoding 259 amino acids.It had 90% homologies with the sequence of potato and 88% homologies with the sequence of tomato. The mature protein isoelectric point was at pH 6.39 and molecular weight was 28.665 kDa.RT-qPCR analysis indicated that SmDREB3 was expressed in all organs.However,the expression level of SmDREB3 was tissue-specific.It expressed highest in the pericarp,followed by the leaf and sarcocarp,while it expressed relatively low in roots,stems and flowers.This study provides the theoretical basis for further researches on resilience mechanisms and cultivating new varieties that resist adverse environment.