紫甘蓝花色苷及其复合体的抗炎活性研究
Anti-inflammatory effects of red cabbage anthocyanin and its complexes

为了探究紫甘蓝花色苷—3-二糖-5-D-吡喃葡糖苷矢车菊素(cyanidin-3-diglcoside-5-glcoside,Cy)及其复合体对鼠源单核-巨噬细胞RAW264.7的抗炎活性,首先采用紫甘蓝花色苷碱水解产物中的Cy为原料,模拟天然蓝花中结构相对稳定的花色苷复合体存在形式,与芦丁(rutin)及2种不同金属离子(Mg2+和Fe3+)构建5种Cy复合体;然后通过建立脂多糖(LPS)诱导的鼠源单核-巨噬细胞RAW264.7炎症模型,评价Cy复合体构建前后对Cy抗炎活性的影响。结果表明,Cy可以显著性抑制RAW264.7细胞上清液中NO释放及细胞内炎症相关基因iNOS、IL-1β、IL-6和TNF-α的mRNA的表达(P<0.05)。在构建的复合体中,与Cy相比,Cy-Fe3+、Cy-rutin-Fe3+、Cy-Mg2+和Cy-rutin-Mg2+复合体的抗炎活性均显著提高(P<0.05)。其中,Cy-Mg2+和Cy-rutin-Mg2+复合体的抗炎活性高于Cy-Fe3+和Cy-rutin-Fe3+复合体,而Cy-Mg2+复合体的抗炎活性最强。由这些结果可以得出Cy及其复合体均具有显著的抗炎活性,且Cy复合体的抗炎活性高于Cy。Cy复合体有望开发成为一种新型食品添加剂,辅助干预炎症反应及其引起的相关疾病。
The aim of this study was to evaluate the anti-inflammatory effects of a kind of red cabbage anthocyanin — cyanidin-3-diglcoside-5-glcoside (Cy) and its complexes on murine RAW264.7 in vitro.Cy was firstly obtained after the alkali hydrolysis of red cabbage anthocyanin.Then,five Cy complexes were constructed with rutin and two metal irons (Mg2+ and Fe3+) imitating the relatively stable existence in blue flowers.Next,the anti-inflammatory effects of Cy and its complexes were evaluated in lipopolysaccharide (LPS)-induced RAW264.7 cells.The results demonstrated that Cy could significantly reduce the release of NO in supernatant and inhibit the relative mRNA expressions of iNOS, IL-1β, TNF-α and IL-6 (P<0.05).Among five Cy complexes,Cy-Fe3+,Cy-rutin-Fe3+,Cy-Mg2+ and Cy-rutin-Mg2+ all presented significant anti-inflammatory effects compared with Cy (P<0.05).Meanwhile,the anti-inflammatory effects of Cy-Mg2+ and Cy-rutin-Mg2+ were better than that of Cy-Fe3+ and Cy-rutin-Fe3+.Furthermore,Cy-Mg2+ presented the best anti-inflammatory effect.In conclusion,both Cy and its complexes presented significant anti-inflammatory effects in vitro.The anti-inflammatory effects of Cy complexes were better than that of Cy,suggesting the potential use as new food additives for the prophylactic or therapeutic purposes of inflammatory reactions and related diseases.