caspase-3限制小鼠脑卒中恢复期齿状回神经前体细胞增殖

Objective To investigate the effect of caspase-3 on the proliferation of neuronal precursor cells (NPCs) in the dentate gyruse (DG) during stroke recovery. Methods The stroke model was produced by electrocoagulating the distal middle cerebral artery (dMCA) of mice, then the NPGs were cultered from ischemia DG on the 7th day after stroke. Treatment groups and control groups were assigned in a randomized manner. The expression of caspase-3 and apoptosis and proliferation of NPCs were analyzed by Western blot, immunocytochemistry, flow cytometry analysis and TUNEL staining in NPCs cultured from ischemic DG. Besides, the effects of caspase-3 inhibition on the proliferation of NPCs in the DG was also investigated by immunohistochemistry in the mouse at 14 day after stroke.Results Caspase-3 was expressed in cultured NPCs,but more than 87% of the caspase-3 positive cells and TUNEL positive cells were not collocated. Caspase-3 negatively regulated self-renewal of NPCs,but did not participate in cell death in culture DG NPCs. Caspase-3 negatively regulated NPCs proliferation in the DG during stroke recovery.Conclusions Caspase-3 may possess a novel function that limits the neurogenic response after stroke.