The
lifetime of G.
biloba is
very long, and its growth is relatively slow. However, little is known about
growth-related genes in this species. We combined mRNA sequencing (RNA-Seq)
with bulked segregant analysis (BSA) to fine map significant agronomic trait
genes by developing polymorphism molecular markers at the transcriptome level.
In this study, transcriptome sequencing of high growth (GD) and low growth (BD)
samples of G.
biloba half-sib families was performed. After assembling the clean reads, 601
differential expression genes were detected and 513 of them were assigned
functional annotations. Single nucleotide polymorphism (SNP) analysis
identified SNPs associated with 119 genes in the GD and BD groups; 58 of these
genes were annotated. Two Homeobox-leucine zipper protein genes were
up-regulated in the GD group compared with the BD group; therefore, these are
very likely related to high growth of G.
biloba.
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