A
method to produce transgenic Camelina sativaplants in cvs. PI650159
and PI650161 was developed. Micropropagated shoot meristemcultures were established
from in vitro germinated seedlings and used as target tissues for Agrobacterium-mediated
transformation. A plasmid harboringenhanced green fluorescent protein,
References
[1]
Budin, J., Breene, W. and Putnam, D. (1995) Some Compositional Properties of Camelina (Camelina sativa L. crantz) Seeds and Oils. Journal of the American Oil Chemists’ Society, 72, 309-315. https://doi.org/10.1007/BF02541088
[2]
Gugel, R.K. and Falk, K.C. (2006) Agronomic and Seed Quality Evaluation of Camelina sativa in Western Canada. Canadian Journal of Plant Science, 86, 1047-1058. https://doi.org/10.4141/P04-081
[3]
Bernardo, A., Howard-Hildige, R., O'Connell, A., Nichol, R., Ryan J., Rice, B., Roche, E. and Leahy, J. (2003) Camelina Oil as a Fuel for Diesel Transport Engines. Industrial Crop and Product, 17, 191-197. https://doi.org/10.1016/S0926-6690(02)00098-5
[4]
Frohlich, A. and Rice, B. (2005) Evaluation of Camelina sativa Oil as a Feedstock for Biodiesel Production. Industrial Crop and Product, 21, 25-31. https://doi.org/10.1016/j.indcrop.2003.12.004
[5]
Choudhury, S.R., Riesselman, A.J. and Pandey, S. (2014) Constitutive or Seed-Specific Overexpression of Arabidopsis G-Protein γ Subunit 3 (AGG3) Results in Increased Seed and Oil Production and Improved Stress Tolerance in Camelina sativa. Plant Biotechnology Journal, 12, 49-59. https://doi.org/10.1111/pbi.12115
[6]
Lee, S.B., Kim, H., Kim, R.J., and Suh, M.C. (2014) Overexpression of Arabidopsis MYB96 Confers Drought Resistance in Camelina sativa via Cuticular Wax Accumulation. Plant Cell Reports, 33, 1535-1546. https://doi.org/10.1007/s00299-014-1636-1
[7]
Miki, B. and McHugh, S. (2004) Selectable Marker Genes in Transgenic Plants: Applications, Alternatives and Biosafety. Journal of Biotechnology, 107, 193-232. https://doi.org/10.1016/j.jbiotec.2003.10.011
[8]
Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987) GUS Fusions: Beta-Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in Higher Plants. The EMBO Journal, 6, 3901-3907. https://doi.org/10.1002/j.1460-2075.1987.tb02730.x
[9]
Narasimhulu, S.B., Kirti, P.B., Bhatt, S.R., Prakash, S. and Chopra, V.L. (1994) Intergeneric Protoplast Fusion between Brassica carinata and Camelina sativa. Plant Cell Reports, 13, 657-660. https://doi.org/10.1007/BF00232940
[10]
Sigareva, M.A. and Earle, E.D. (1999) Camalexin Induction in Intertribal Somatic hybrids between Camelina sativa and Rapid-Cycling Brassica oleracea. Theoretical and Applied Genetics, 98, 164-170. https://doi.org/10.1007/s001220051053
[11]
Tattersall, A. and Millam, S. (1998) Establishment and in Vitro Regeneration Studies of the Potential Oil Crop Species Camelina sativa. Plant Cell Tissue Organ Culture, 55, 147-150. https://doi.org/10.1023/A:1006132407886
[12]
Kuvshinov, V., Kanerva, A., Koivu, K., Kuvshinova, S. and Pehu, E. (2009) Transformation System for Camelina sativa. Patent # 12/290,379, USA.
[13]
Liu, L., Liu, Z., Bai, H. and Sun, D.D. (2012) Concurrent Filtration and Solar Photocatalytic Disinfection/Degradation Using High-Performance Ag/TiO2 Nanofiber Membrane. Water Resources, 46, 1101-1112.
[14]
Petrie, J.R., Shrestha, P., Belide, S., Kennedy, Y., Lester, G., Liu Q., Divi, U.K., Mulder, R. J., Mansour, M.P., Nichols, P.D. and Singh, S.P. (2014) Metabolic Engineering of Camelina sativa with Fish Oil-Like Levels of DHA. PLoS ONE, 9, e85061. https://doi.org/10.1371/journal.pone.0085061
[15]
Zhu, Y., Xie, L., Chen, G.Q., Lee, M.Y., Loque, D. and Scheller, H.V. (2018) A Transgene Design for Enhancing Oil Content in Arabidopsis and Camelina Seeds. Biotechnology for Biofuels, 11, No. 1. https://doi.org/10.1186/s13068-018-1049-4
[16]
Kanerva, A., Koivu. K., Kuvshinov. V., Kuvshinova, S. and Eija, P. (2011) Transformation in Camelina sativa. Patent # US7910803B2, USA.
[17]
Yemets, A.I., Boychuk, Y.N., Shysha, E.N., Rakhmetov, D.B. and Blume, Y.B. (2013) Establishment of in Vitro Culture, Plant Regeneration, and Genetic Transformation of Camelina sativa. Cytology and Genetics, 47, 14-20. https://doi.org/10.3103/S0095452713030031
[18]
Sitther V., Tabatabai, B., Enitan, O. and Dhekney, S. (2018) Agrobacterium-Mediated Transformation of Camelina sativa for Production of Transgenic Plants. Journal of Biological Methods, 5, e83. https://doi.org/10.14440/jbm.2018.208
[19]
Dutt, M., Li, T., Dhekney, A. and Gray, J. (2007) Transgenic Plants from Shoot Apical Meristems of Vitis vinifera L. “Thompson Seedless” via Agrobacterium-Mediated Transformation. Plant Cell Reports, 26, 2101-2110. https://doi.org/10.1007/s00299-007-0424-6
[20]
Li, T., Dhekney, A., Dutt, M. and Gray, J. (2008) An Improved Protocol for Agrobacterium-Mediated Transformation of Grapevine (Vitis vinifera L.). Plant Cell, Tissue and Organ Culture, 93, 311-321. https://doi.org/10.1007/s11240-008-9378-9
[21]
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
[22]
Dhekney, A., Li, T., Dutt, M. and Gray, J. (2012) Initiation and Transformation of Grapevine Embryogenic Cultures. In: Transgenic Plants, Humana Press, New York, 215-225. https://doi.org/10.1007/978-1-61779-558-9_18
[23]
Dhekney, A., Li, T., Zimmerman, W. and Gray, J. (2009) Factors Influencing Genetic Transformation and Plant Regeneration of Vitis. American Journal of Enology and Viticulture, 60, 285-292.
[24]
Lu, C. and Kang, J. (2008) Generation of Transgenic Plants of a Potential Oilseed Crop Camelina sativa by Agrobacterium-Mediated Transformation. Plant Cell Reports, 27, 273-278. https://doi.org/10.1007/s00299-007-0454-0
[25]
Haggins, K.J., Potlakayala, S., Hussain, I., Josekutty, P.C. and Rudrabhatla, S. (2011) Improving Stress Tolerance in Camelina sativa. NSF-REU Symposium, Penn State Harrisburg.
[26]
Barlass, M. and Skene, K.G.M. (1978) In Vitro Propagation of Grapevine (Vitis vinifera L) from Fragmented Shoot Apices. Vitis, 17, 335-340.
[27]
Dutt, M. and Grosser, J.W. (2009) Evaluation of Parameters Affecting Agrobacterium-Mediated Transformation of Citrus. Plant Cell Tissue Organ Culture, 98, 331-340. https://doi.org/10.1007/s11240-009-9567-1