Phytochemical and Antifungal Activity of Leaf Extracts of Prosopis africana and Anacardium occidentale against Macrophomina Root Rot of Sesamum indicum L. in Benue State, Central Nigeria
This study investigated the antifungal activity of leaf extracts of Prosopis africana and Anacardium occidentale against Macrophomina phaseolina, the causal agent of root rot of Sesamum indicum L. Phytochemical analysis of the two plants showed the presence of alkaloids, saponins, tannins, flavonoids and anthraquinones in petroleum ether, ethyl acetate, methanol and water extracts. The effectiveness of the two medicinal plants viz: P. africana and A. occidentale was tested against the causative agent of root rot of Sesamum indicum L. The effect of plant leaf extracts on mycelia growth of the test organism shows that both P. africana and A. anacardium reduced the mycelia growth significantly as compared to the control (plate, 2, 3, 4). The antifungal property of P. africana and A. occidentale makes these plants of potential interest for the control of the fungi Macrophomina phaseolina.
References
[1]
[1] Mohammad, D.H. and Merajul, I.R. (2015) In Vitro Studies on Phytochemical Screening of Different Leaf Extracts and Their Antifungal Activity against Seed Borne Pathogen Aspergillus niger. Journal of Plant Pathology & Microbiology, 6, 320.
[2]
Elaigwu, M., Oluma, H.A.O. and Ochokwunu, D. (2017) In Vivo and In Vitro Activities of Some Plant Extracts on Macrophomina Phaseolina (Tassi) Goid. The Casual Agent of Charcoal Rot of Sesame in Benue State, Nigeria. IJSRM, 5, No. 4.
[3]
Oluma, H.O.A. and Elaigwu, M. (2006) Antifungal Activity of Extracts of Some Medicinal Plants against Macrophomina phaseolina (Tassi). Goid. Nigeria Journal of Botany, 19, 121-128.
[4]
Akash, P.D., Vishal, D.J. and Arun, B.J. (2009) Antimicrobial Screening of Different Extract of Anacardium occidentale Linn. Leaves. Journal of ChemTech Research, 1, 856-858.
[5]
Ezike, A.C., Akah, P.A., Okoli, C.O., Udegbunam, S., Okwume, N., Okeke, C. and Iloani, O. (2010) Medicinal Plants Used in Wound Care: A Study of Prosopis africana (Fabaceae) Stem Bark. Indian Journal of Pharmaceutical Sciences, 72, 3349 p.
[6]
Patro, C. and Behera, R.N. (1979) Cashew Helps to Fix sand Dunes in Orissa. Indian Farming, 28, 31-32.
[7]
Shokalu, O., Enikuomehin O.A., Idowu, A.A. and Uwala, A.C. (2002) Effects of Seed Treatment on the Control of Leaf Blight Disease of Sesame. Tropical Oilseeds Journal, 7, 94-100.
[8]
Hayes, W.J. and Laws, E.R. (1991) Handbook of Pesticide Toxicology. Academic Press Inc., New Delhi, 25-30.
[9]
Enikuomehin, O.A. and Peters, O.T. (2002) Evaluation of Crude Extracts from Some Nigerian Plants for the Control of Field Diseases of Sesame (Sesamum indicum L.). Tropical Oilseeds Journal, 7, 84-93.
[10]
Okigbo, R.N. and Emoghene, A.O. (2003) Effect of Leaf Extract of Three Plant Species on Mycosphaerella fijiensis Morelet, the Causal Organism of Black Sigatoka Disease of Banana (Musa acuminata). Nigerian Journal of Plant Protection, 20, 101-110.
[11]
Oluma, H.O.A. and Garba, I.U. (2004). Screening of Eucalyptus globulus and Ocimum gratissimum against Pythium aphanidermatum. Nigeria Journal of Plant Protection, 21, 109-114.
[12]
Sofowora, A. (1993) Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd., Ibadan.
[13]
Trease, G.E. and Evans, W.C. (1989) Pharmacognosy: A Physician’s Guide to Herbal Medicine. 13th Edition, Bailiere Tindall, London, 912.
[14]
Tewari, S.N. and Nayak, M. (1999) Activity of Four Plant Extracts against Three Fungal Pathogens of Rice. Tropical Agriculture (Trinidad), 68, 373-375.
[15]
Amadioha, A.C. and Obi, V.I. (1999) Control of Anthracnose Disease of Cowpea by Cympopogon citratus and Ocimum gratissimum. Acta Phytopathologica et Entomologica Hungarica, 34, 85-89.
[16]
Mota, M.L., Thomas, G. and Barbosa-filho, J.M. (1985) Anti-Inflammatory Actions of Tannins Isolated from the Bark of Anacardium occidentale L. Journal of Ethnopharmacology, 13, 289-300. https://doi.org/10.1016/0378-8741(85)90074-1
[17]
Laurens (1982) Study of Antimicrobial Activity of Anarcadiun occidentale. Annales Pharmaceutiques Françaises, 40, 143-146.
[18]
Kudi, A.C., Umoh, J.U. and Eduve, L.E. (1999) Screening of Some Nigeria Medicinal Plants for Antibacterial Activity. Journal of Ethnopharmacology, 69, 225-228. https://doi.org/10.1016/S0378-8741(98)00214-1
[19]
Omojasola, P.F. and Awe, S. (2004) The Antibacterial Activity of the Leaf Extracts of Anacardiun occidentale and Gossypium hirsutun against Some Selected Microorganisms. Bioscience Research Communications, 60, 25-58.
[20]
Lale, N.E.S. (2002) Bio-Activity and Limitation against Wide Spread Use of Neem Products for the Management of Insect Pests. Nigerian Journal of Experimental and Applied Biology, 3, 115-125.
[21]
Tewari, S. and Singh, A. (2004) Toxic and Sub Lethal Effects Oleadrin on Biochemical Parameters of Freshwater Air Breathing Murrel. Chant Punctatus (Bloch.). Indian Journal of Experimental Biology, 42, 413-418.
[22]
Okorie, J.M., Okonkwo, T.J. and Okonta, N. (2010) Sub-Chronic Hepatoxicity of Anarcadium occidentale. Indian Journal of Pharmacology Science, 72, 353-357.
[23]
Arekemase, M.O., Oyeyiola, G.P. and Aliyu, M.B. (2011) Antibacterial Activity of Anacardium occidentale on Some Enterotoxin Producing Bacteria. International Journal of Biology, 3, 92-97. https://doi.org/10.5539/ijb.v3n4p92
[24]
Rajesh, K.V., Sumathi, C.S., Balasubramanian, V. and Ramesh, N. (2009) Elementary Chemical Profiling and Antifungal Properties of Cashew (Anacardium occidentale L.) Nuts. Botany Research International, 2, 253-257.
[25]
Rubenice, A.S., Silvana, A.L., Flavia, M., Amaral, F.R., Fernandes, N., Luce, M.B., Valério, M.N. and Rosane, N.M.G. (2016) Antimicrobial and Antioxidant Activity of Anacardium occidentale L. Flowers in Comparison to Bark and Leaves Extracts. Journal of Biosciences and Medicines, 4, 87-99.
[26]
Tapia, A., Egly, F.G., Bustos, D., Astudillo, L., Theoduloz, C. and Schmeda-Hirschmann, G. (2000) Biologically Active Alkaloids and a Free Radical Scavenger from Prosopis Species. Journal of Ethnopharmacology, 71, 241-246. https://doi.org/10.1016/S0378-8741(00)00171-9
[27]
Rwang, P.G., Fabiyi, J.P., Suleiman, M. and Mercy, K.P. (2016) Evaluation and Phytochemical Analysis of Prosopis africana and Erythrina senegalensis Used against Immature Stages of Schistosoma haematobium. European Journal of Medicinal Plants, 13, 1-9. https://doi.org/10.9734/EJMP/2016/17675
[28]
Badamasi, M.Z., Adamu, T.U., Abubakar, U., Ladan, M.J. and Bala, A.Y. (2011) Antimalarial Activity and Phytochemical Analysis of the Aqueous Stem Bark Extract of Prosopis africana (Kirya) against Plasmodium Species. Nigerian Journal of Parasitology, 32, 271-275.
[29]
Arshad, J. and Rehman, H.A. (2011) Antifungal Activity of Leaf Extracts of Some Medicinal Trees against M. phaseolina. Journal of Medicinal Plants Research, 5, 2868-2872.
[30]
Jabeen, K. and Javid, A. (2008) Antifungal Activity of Aqueous and Organic Solvent Extracts of Allelopathic Trees against Ascochyta rabiei. Allelopathy Journal, 22, 231-238.
[31]
Prince, L. and Prabakaran, P. (2011) Antifungal Activity of Medicinal Plants against Plant Pathogenic Fungus Colletotrichum falcatum. Asian Journal of Plant Science and Research, 1, 84-87.
[32]
Shadab, Q., Hanif, M. and Chaudhary, F.M. (1992) Antifungal Activity by Lemongrass Essential Oils. Pakistan Journal of Scientific and Industrial Research, 35, 246-249.
[33]
Kareru, P.G., Keriko, J.M., Kenji, G.M. and Gachanja, A.N. (2010) Anti-Termite and Antimicrobial Properties of Paint Made from Thevetia peruviana (Pers.) Schum. Oil Extract. African Journal of Pharmacy and Pharmacology, 4, 87-89.
[34]
Ogunbosoye, D.O. and Babayemi, O.J. (2010) Potential Values of Some Non-Leguminous Browse Plants as Dry Season Feed for Ruminants in Nigeria. African Journal of Biotechnology, 9, 2720-2726.
[35]
Banso, A. and Adeyemo, S.O. (2007) Evaluation of Antimicrobial Properties of Tannins Isolated from Dichrostachys cinereaa. African Journal of Biotechnology, 6, 1785-1787. https://doi.org/10.5897/AJB2007.000-2262