The potential of Near Infrared Spectroscopy (NIRS) in quality control of Traditional Chinese Medicine has been evaluated. This study focuses on a rapid and non-destructive quantitative method for the determination of schaftoside acid content in Desmodium styracifolium by near-infrared spectroscopy. The schaftoside acid contents of 103 samples were determined by HPLC. The NIRS quantitative model of the ethanol extracts content was performed by partial least-squares (PLS) regression as linear regression with analysis values as calibration reference using 2nd derivative method as spectral preprocessing options. The model was verified by internal cross validation and external predictive validation with 22 Desmodium styracifolium samples. The correlation coefficients (r), the root-mean-square error of calibration (RMSEC), the root-mean-square error of prediction (RMSEP) and the root-mean-square error of cross-validation (RMSECV) of the calibration model for ethanol extracts content were 0.97201, 0.147, 0.162 and 0.433, respectively. The average recovery was 99.82%, and t-test showed that P > 0.5. Precision and repeatability RSD were 1.75% and 1.17%, RPD > 2.5. The data obtained from results showed that calibration model of NIRS was verified to be reliable, suggesting that the quantitative NIR models can be qualified to accurately quantify the ethanol extracts content of Desmodium styracifolium rapidly.
References
[1]
Liu, L., Chen, M. and Chen, X. (2015) Analysis of Alcohol Dehydrogenase Inhibitors from Desmodium Styracifolium Using Centrifugal Ultrafltration Coupled with HPLC-MS. Journal of the Serbian Chemical Society, 80, 23-23.
https://doi.org/10.2298/JSC140919023L
[2]
Moe, O.W. (2006) Kidney Stones: Pathophysiology and Medical Management. Lancet, 367, 333-344. https://doi.org/10.1016/S0140-6736(06)68071-9
[3]
Gürocak, S. and Küpeli, B. (2006) Consumption of Historical and Current Phytotherapeutic Agents for Urolithiasis: A Critical Review. Journal of Urology, 176, 450-455. https://doi.org/10.1016/j.juro.2006.03.034
[4]
Xiang, S.T., Zhou, J.F., Gan, S., Rong, X.L., Li, J. and Wang, S.S. (2013) Antiurolithiatic Activity of Aqueous Extract from Desmodium styracifolium (Osb.) Merr. on Renal Calcium Oxalate in Rats. Chinese Journal of Experimental Surgery, 30, 1166-1166.
[5]
Xiang, S.T., Gan, S., Zhou, J.F., et al. (2014) Effects of Aqueous Extract from Desmodium styracifolium (Osb.) Merr. on Oxidative Stress of Renal Calcium Oxalate Rat Models. Chinese Journal of Urology, 35, 465-468.
[6]
Zhang, Y.Q., Luo, J.G., Han, C., Xu, J.F. and Kong, L.Y. (2015) Bioassay-Guided Preparative Separation of Angiotensin-Converting Enzyme Inhibitory C-Flavone Glycosides from Desmodium styracifolium by Recycling Complexation High-Speed Counter-Current Chromatography. Journal of Pharmaceutical & Biomedical Analysis, 102, 276-281. https://doi.org/10.1016/j.jpba.2014.09.027
[7]
Committee, N.P. (2015) Pharamcoeia of People’s Republic of China (Part 1). Chemical Industry Press, Beijing.
[8]
Guo, N.X., Cai, J.L., Huang, J.Y., Li, L.L. and Ji, S.G. (2013) Quantitative Model for Hesperidin Content in Citrus Reticulate by NIR Spectroscopy. China Pharmacy, 24, 2932-2934.
[9]
Li, L.L., Wang, H.X., Ling, P. and Ji, S.G. (2013) Application of Near-Infrared Spectroscopy in Determination of Moisture Content in Eriobotrya Japonica. Chinese Journal of Experimental Traditional Medical Formulae, 19, 104-109.
[10]
Wang, D., Jia, Y. and Ji, S.G. (2013) Identification of Dihuang Steamed for Different Hours with Near-Infrared Spectrum. Chinese Journal of Spectroscopy Laboratory, 27, 1356-1360.
[11]
Kirchler, C.G., Pezzei, C.K., Beć, K.B., Henn, R., Ishigaki, M., Ozaki, Y., et al. (2017) Critical Evaluation of NIR and ATR-IR Spectroscopic Quantifications of Rosmarinic Acid in Rosmarini Folium Supported by Quantum Chemical Calculations. Planta Medica, 83, 1076-1084. https://doi.org/10.1055/s-0043-107032
[12]
Li, W., Wang, Y. and Qu, H. (2012) Near Infrared Spectroscopy as a Tool for the Rapid Analysis of the Honeysuckle Extracts. Vibrational Spectroscopy, 62, 159-164.
https://doi.org/10.1016/j.vibspec.2012.07.005
[13]
Pezzei, C.K., Schönbichler, S.A., Hussain, S., Kirchler, C.G., Huck-Pezzei, V.A., Popp, M., Krolitzek, J., Bonn, G.K. and Huck, C.W. (2017) Near-Infrared and Mid-Infrared Spectroscopic Techniques for a Fast and Non-Destructive Quality Control of Thymi Herba. Planta Medica. https://doi.org/10.1055/s-0043-121038
[14]
Nie, L., Dai, Z. and Ma, S. (2016) Enhanced Accuracy of Near-Infrared Spectroscopy for Traditional Chinese Medicine with Competitive Adaptive Reweighted Sampling. Analytical Letters, 49, 2259-2267.
https://doi.org/10.1080/00032719.2016.1143479
