Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared
by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with
the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk.
The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC
solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured
from images of transmission electron microscopy (TEM). The effect of foliar
application of the mixture of OC-nSiO2 on the induction of resistance against
anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili
fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal
treatment that reduced the disease severity on chili fruits to 22.2% compared
with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered
as an effective biotic elicitor to prevent anthracnose disease infection for
chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be
used as an environmentally friendly agrochemical product for sustainable development
of agriculture.
References
[1]
Kim, K.W. and Thomas, R.L. (2007) Antioxidative Activity of Chitosans with Varying Molecular Weights. Food Chemistry, 101, 308-313.
https://doi.org/10.1016/j.foodchem.2006.01.038
[2]
Zheng, L.Y. and Zhu, J.F. (2003) Study on Antimicrobial Activity of Chitosan with Different Molecular Weights. Carbohydrate Polymers, 54, 527-530.
https://doi.org/10.1016/j.carbpol.2003.07.009
[3]
Chamnanmanoontham, N., Pongprayoon, W., Pichayangkura, R., Roytrakul, S. and Chadchawan, S. (2015) Chitosan Enhances Rice Seedling Growth via Gene Expression Network between Nucleus and Chloroplast. Plant Growth Regulation, 75, 101-114. https://doi.org/10.1007/s10725-014-9935-7
[4]
Dzung, N.A., Khanh, V.T.P. and Dzung, T.T. (2011) Research on Impact of Chitosan Oligomers on Biophysical Characteristics, Growth, Development and Drought Resistance of Coffee. Carbohydrate Polymers, 84, 751-755.
https://doi.org/10.1016/j.carbpol.2010.07.066
[5]
Luan, L.Q., Nagasawa, N., Tamada, M. and Nakanishi, T.M. (2006) Enhancement of Plant Growth Activity of Irradiated Chitosan by Molecular Weight Fractionation. Radioisotopes, 55, 21-27. https://doi.org/10.3769/radioisotopes.55.21
[6]
Aziz, A., Trotel-Aziz, P., Dhuicq, L., Jeandet, P., Couderchet, M., et al. (2006) Chitosan Oligomers and Copper Sulfate Induce Grapevine Defense Reactions and Resistance to Gray Mold and Downy Mildew. Phytopathology, 96, 1188-1194.
https://doi.org/10.1094/PHYTO-96-1188
[7]
Vasyukova, N.I., Zinov’eva, S.V., Il’inskaya, L.I., Perekhod, E.A., Chalenko, G.I., et al. (2001) Modulation of Plant Resistance to Diseases by Water-Soluble Chitosan. Applied Biochemistry and Microbiology, 37, 103-109.
https://doi.org/10.1023/A:1002865029994
[8]
Yin, H., Zhao, X. and Du, Y. (2010) Oligochitosan: A Plant Disease Vaccine: A Review. Carbohydrate Polymers, 82, 1-8. https://doi.org/10.1016/j.carbpol.2010.03.066
[9]
Garg, A. and Balodi, R. (2014) Recent Trends in Agriculture: Vertical Farming and Organic Farming. Advances in Plants and Agriculture Research, 1, Article ID: 00023. https://doi.org/10.15406/apar.2014.01.00023
[10]
Stockdale, E.A., Lampkin, N.H., Hovi, M., Keatinge, R., Lennartsson, E.K.M., et al. (2001) Agronomic and Environmental Implications of Organic Farming Systems. Advances in Agronomy, 70, 263-327.
https://doi.org/10.1016/S0065-2113(01)70007-7
[11]
Huang, S., Wang, L., Liu, L., Hou, Y. and Li, L. (2015) Nanotechnology in Agriculture, Livestock, and Aquaculture in China. A Review. Agronomy for Sustainable Development, 35, 369-400. https://doi.org/10.1007/s13593-014-0274-x
[12]
Taha, R.A. (2016) Nanotechnology and Its Application in Agriculture. Advances in Plants and Agriculture Research, 3, Article ID: 00089.
https://doi.org/10.15406/apar.2016.03.00089
[13]
Kiirika, L.M., Stahl, F. and Wydra, K. (2013) Phenotypic and Molecular Characterization of Resistance Induction by Single and Combined Application of Chitosan and Silicon in Tomato against Ralstonia solanacearum. Physiological and Molecular Plant Pathology, 81, 1-12. https://doi.org/10.1016/j.pmpp.2012.11.002
[14]
Tiwari, R.K. (n.d.) Post Harvest Profile of Chili.
http://agmarknet.gov.in/Others/preface-chhilli.pdf
[15]
Huq, A.S.M.A. and Arshad, F.M. (2010) Technical Efficiency of Chili Production. American Journal of Applied Sciences, 7, 185-190.
https://doi.org/10.3844/ajassp.2010.185.190
[16]
Mahasuk, P., Khumpeng, N., Wasee, S., Taylor, P.W.J. and Mongkolporn, O. (2009) Inheritance of Resistance to Anthracnose (Colletotricum casici) at Seedling and Fruiting Stages in Chili Pepper (Capsicum spp.). Plant Breeding, 128, 701-706.
https://doi.org/10.1111/j.1439-0523.2008.01615.x
[17]
Duy, N.N., Phu, D.V., Anh, N.T. and Hien, N.Q. (2011) Synergistic Degradation to Prepare Oligochitosan by γ-Irradiation of Chitosan Solution in the Presence of Hydrogen Peroxide. Radiation Physics and Chemistry, 80, 848-853.
https://doi.org/10.1016/j.radphyschem.2011.03.012
[18]
Dzung, P.D, Ngoc, L.S., Thuy, N.N., Truc, L.T.M., Le, B.V., et al. (2016) Effect of Nanosilica from Rice Husk on the Growth Enhancement of Chili Plant (Casicum frutescens L.). Journal of Science and Technology, 54, 607-613.
[19]
Tiraferri A., Maroni, P., Rodríguez, D.C. and Borkovec, M. (2014) Mechanism of Chitosan Adsorption on Silica from Aqueous Solutions. Langmuir, 30, 4980-4988.
https://doi.org/10.1021/la500680g
[20]
Somapala, K.S., Weerahewa, H.L.D. and Thrikawala, S. (2016) Enhanced Resistance against Anthracnose Disease in Chili Pepper (Capsicum annuum L.) by Soil Application of Potassium. International Journal of Agriculture, Forestry and Plantation, 2, 35-39.
http://digital.lib.ou.ac.lk/docs/bitstream/701300122/1227/1/KLIAFP2_39.pdf
[21]
Ahmad, A. and Ashraf, Y. (2016) In Vitro and In Vivo Management of Alternaria Leaf Spot of Brassica campestris L. Journal of Plant Pathology & Microbiology, 7, Article ID: 1000365. https://doi.org/10.4172/2157-7471.1000365
[22]
Das, S.N., Madhuprakash, J., Sarma, P.V.S.R.N., Purushotham, P., Suma, K., et al. (2015) Biotechnological Approaches for Field Applications of Chitooligosaccharides (COS) to Induce Innate Immunity in Plants. Critical Reviews in Biotechnology, 35, 29-43. https://doi.org/10.4172/2157-7471.1000365
[23]
Phu, D.V., Du, B.D., Tuan, L.N.A., Tam, H.V. and Hien, N.Q. (2017) Preparation and Foliar Application of Oligochitosan-Nanosilica on the Enhancement of Soybean Seed Yield. International Journal of Environment, Agriculture and Biotechnology, 2, 421-428. https://doi.org/10.22161/ijeab/2.1.53
[24]
Sumarni, W., Iswari, R.S., Marwoto, P. and Rahayu, E.F. (2016) Physical Characteristics of Chitosan-Silica Composite of Rice Husk Ash. IOP Conference Series: Materials Science and Engineering, 107, Article ID: 012039.
https://doi.org/10.1088/1757-899X/107/1/012039
[25]
Ghormade, V., Deshpande, M.V. and Paknikar, K.M. (2011) Perspective for Nano-Biotechnology Enabled Protection and Nutrition of Plants. Biotechnology Advances, 29, 792-803. https://doi.org/10.1016/j.biotechadv.2011.06.007
[26]
Fruijtier-Pölloth, C. (2012) The Toxicological Mode of Action and the Safety of Synthetic Amorphous Silica-A Nanostructured Material. Toxicology, 294, 61-79.
https://doi.org/10.1016/j.tox.2012.02.001
