Photodynamic therapy today is becoming an important role in the healing of lacerated tissues, since it has therapeutic resources capable of accelerating this process. One treatment option is the clinical phototherapy, and Photodynamic Therapy (PDT) is being widely used. This study aims to evaluate the effect of PDT on the healing of skin wounds in rats. We used a sample of 39 male rats Wistar divided into three groups, a control, a PDT-treated green and red with the last PDT. After 24 hours before the surgical incisions, PDT was used in both groups for 6 minutes and was evaluated in histological level, the inflammatory reaction and the repair process. The results showed that the granulation tissue was more developed in the irradiated group than in the control group and the amount of chronic inflammatory cells (monocytes, macrophages, lymphocytes and plasma cells) predominated with green phototherapy. The epithelialization in the wound margins and scarring with better quality occurred with red PDT (640 nm), which the higher deposition was of collagen. However, phototherapy not collimated of 640 nm (red) resulted in better anti-inflammatory effects. Given these answers with phototherapy not collimated green (525 nm) and red (640 nm), we conclude that the use of these wavelengths is possible with benefits, mainly with red LED.
References
[1]
Alwan, A., Maclean, D.R., Riley, L.M., d’Espaignet, E.T., Mathers, C.D., Stevens, G.A. and Bettcher, D. (2010) Monitoring and Surveillance of Chronic Noncommunicable Diseases: Progress and Capacity in High Burden Countries. The Lancet, 376, 1861-1868. https://doi.org/10.1016/S0140-6736(10)61853-3
[2]
Fang, R.C. and Galiano, R.D. (2009) Adjunctive Therapies in the Treatment of Osteomyelitis. Seminars in Plastic Surgery, 23, 141-147. https://doi.org/10.1055/s-0029-1214166
[3]
Panuncialman, J. and Falanga, V. (2010) Unusual Causes of Cutaneous Ulceration. Surgical Clinics of North America, 90, 1161-1180.
[4]
Hamblin, M.R. (2016) Antimicrobial Photodynamic Inactivation: A Bright Newtechnique to Kill Resistant Microbes. Current Opinion in Microbiology, 33, 67-73. https://doi.org/10.1016/j.mib.2016.06.008
[5]
Diretrizes da Sociedade Brasileira de Diabetes (2016) Parte 2: Complicacoes, Doencas Relacionadas e Decorrentes do Diabetes Mellitus. 137-214. http://www.diabetes.org.br/sbdonline/images/docs/DIRETRIZES-SBD-2015-2016.pdf
[6]
Darlenski, R. and Fluhr, J.W. (2012) Photodynamic Therapy in Dermatology: Past, Present, and Future. Journal of Biomedical Optics, 18, Article ID: 061208. https://doi.org/10.1117/1.jbo.18.6.061208
[7]
Kashef, N., Djavid, G.E., Siroosy, M., Khani, A.T., Zokai, F.H. and Fateh, M. (2011) Photodynamic Inactivation of Drug-Resistant Bacteria Isolated from Diabetic Foot Ulcers. Iranian Journal of Microbiology, 3, 36-41.
[8]
Abrahamse, H. and Hamblin, M.R. (2016) New Photosensitizers for Photodynamic Therapy. Biochemical Journal, 473, 347-364. https://doi.org/10.1042/BJ20150942
[9]
Lei, X., Liu, B. and Wu, J. (2015) A Clinical Study of Photodynamic Therapy for Chronic Skin Ulcersin Lower Limbs Infected with Pseudomonas aeruginosa. Archives of Dermatological Research, 307, 49-55. https://doi.org/10.1007/s00403-014-1520-4
[10]
Sharma, M., Visai, L., Bragheri, F., Cristiani, I., Gupta, P.K. and Speziale, P. (2008) Toluidine Blue-Mediated Photodynamic Effects on Staphylococcal Biofilms. Antimicrobial Agents and Chemotherapy, 52, 299-305. https://doi.org/10.1128/AAC.00988-07
[11]
Tardivo, J.P. and Baptista, M.S. (2009) Treatment of Osteomyelitis in the Feet of Diabetic Patients by Photodynamic Antimicrobial Chemotherapy. Photomedicine and Laser Surgery, 145-150. https://doi.org/10.1089/pho.2008.2252
[12]
Tardivo, J.P., Adami, F., Correa, J.A., Pinhal, M.A.S. and Baptista, M.S. (2014) A Clinical Trial Testing the Efficacy of PDT in Preventing Amputation in Diabetic Patients. Photodiagnosis and Photodynamic Therapy, 11, 342-350. https://doi.org/10.1016/j.pdpdt.2014.04.007
[13]
Abramovitis, W. and Arrozala, A.G.K. (2005) Light-Emitting Diode-Based Therapy. Skinmed, 4, 38-41. https://doi.org/10.1111/j.1540-9740.2005.03959.x
[14]
Meyer, P.F., Araújo, H.G., Carvalho, M.G.F., Tatum, B.I.S., Fernandes, I.C.A., Ronzio, O.A. and Pinto, M.V.M. (2010) Avaliacao dos efeitos do LED na cicatrizacao de feridas cutaneas em ratos Wistar. Fisioterapia Brasil, 11, 428-432.
[15]
Failache, H. and Geido, D. (2006) Fonte de fototerapia em base a LEDs, Facultades de Ingeniería y Medicina Universidad de la República Del Uruguay. Montevidéu. http://uruguayescribe/fuente-de-luz-azul-para-el-tratamiento-de-la-hiperbilirr ubinemia-neonatal
[16]
Pinto, M.V.M. (2011) Fototerapia Aspectos Clínicos da Reabilitacao. Editora Andreoli.
[17]
Jorge, A.S. and Dantas, S.R.P.E. (2003) Abordagem multiprofissional do tratamento de feridas. Atheneu, Sao Paulo.
[18]
Vinck, E.M., Cagnie, B.J., Cornelissen, M.J., Declercg, H.A. and Cambier, D.C. (2005) Green Light Emitting Diode Irradiation Enhances Fibroblast Growth Impaired by High Glucose Level. Photomed Laser Surg, 2, 167-171. https://doi.org/ 10.1089/pho.2005.23.167
[19]
Carvalho, P.T.C. (2002) Análise da cicatrizacao de lesoes cutaneas através da espectrofometria: Estudo experimental em ratos diabéticos [Dissertacao]. Universidade de Sao Paulo, Sao Paulo.
[20]
Seung, A., Park, K.H., Choi, J.W., Kwon, J.K., Doo, K.L., Mi, S.S., et al. (2007) A Prospective, Randomized, Placebo-Controlled, Double-Blinded, and Split-Face Clinical Study on LED Phototherapy for Skin Rejuvenation: Clinical, Profilometric, Histologic, Ultrastructural, and Biochemical Evaluations and Comparison of Three Different Treatment Settings. Journal of Photochemistry and Photobiology, 88, 51-67. https://doi.org/10.1016/j.jphotobiol.2007.04.008
[21]
Trelles, A.M. and Allones, E.M. (2006) Red Light-Emitting Diode (LED) Therapy Accelerates Wound Healing Post-Blepharoplasty and Periocular Laser Ablative Resurfacing. Journal of Cosmetic and Laser Therapy, 8, 39-42. https://doi.org/10.1080/14764170600607731
[22]
Pinto, M.V.M., Costa, D.A., Aguiar, J.L.N., Barauna, M.A., Silva, A.L.S., Biagini, A.P., et al. (2007) Análise do efeito da terapia laser de baixa intensidade e do controle doloroso na cicatrizacao da úlcera venosa. Revista Dor, 8, 1113-1137.
[23]
Pinto, M.V.M., Costa, D.A., Rocha, L.L.V., Santos, H.R., Silva, A.L.S.S., Barbosa, L.G., et al. (2008) Estudo comparativo dos efeitos do Ga-As (904 nm, 150mW) laser e do ultra-som pulsado de 1 MHz na inflamacao do músculo tibial de ratos Wistar. Brazilian Archives of Biology and Technology, 51, 225-230.
[24]
Bartoled, D. (2008) Light-Emitting Diodes (LEDs) in Dermatology. Seminars in Cu- taneous Medicine and Surgery, 27, 227-238. https://doi.org/10.1016/j.sder.2008.08.003
