All Title Author
Keywords Abstract

Thiol-Disulfide Homeostasis in Patients with Panic Disorder

DOI: 10.4236/ijcm.2017.81004, PP. 34-41

Keywords: Panic Disorder, Thiol-Disulphide Homeostasis

Full-Text   Cite this paper   Add to My Lib


Objective: To determine serum thiol/disulfide homeostasis in panic disorder (PD). Methods: Serum native thiol, total thiol, and disulfide levels were measured in the patients with 40 PD patients and 40 healthy subjects. Serum native thiol, total thiol, and disulfide levels were measured with a novel colorimetric, automated method. The thiol-disulfide ratio was also calculated. Results: The native thiol (p < 0.001) and total thiol (p < 0.001) levels, and the native thiol/total thiol (p < 0.001) ratio were significantly lower, whereas disulfide/native thiol (p < 0.001) and disulfide/total thiol (p < 0.001) ratio significantly increased in the PD patient group compared to the control group. The cut-off value was 92.26, 3.83 and 3.56 for native thiol/total thiol, disulfide/native thiol and disulfide/total thiol respectively. Conclusion: This is the first study in the literature to evaluate thiol-disulfide homeostasis in patients with PD. Our results suggest that the disulfide/thiol ratio is significantly greater in panic disorder patients.


[1]  Ersoy, M.A., Selek, S., Celik, H., Erel, O., Kaya, M.C., Savas, H.A., et al. (2008) Role of Oxidative and Antioxidative Parameters in Etiopathogenesis and Prognosis of Panic Disorder. International Journal of Neuroscience, 118, 1025-1037.
[2]  Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., et al. (2014) Oxidative Stress, Prooxidants, and Antioxidants: The Interplay. BioMed Research International, 2014, Article ID: 761264.
[3]  Gul, I.G., Karlidag, R., Cumurcu, B.E., Turkoz, Y., Kartalci, S., Ozcan, A.C., et al. (2013) The Effect of Agoraphobia on Oxidative Stress in Panic Disorder. Psychiatry Investigation, 10, 317-325.
[4]  Bouayed, J., Rammal, H. and Soulimani, R. (2009) Oxidative Stress and Anxiety: Relationship and Cellular Pathways. Oxidative Medicine and Cellular Longevity, 2, 63-67.
[5]  Cadenas, E. (1989) Biochemistry of Oxygen Toxicity. Annual Review of Biochemistry, 58, 79-110.
[6]  Tetik, S., Ahmad, S., Alturfan, A.A., Fresko, I., Disbudak, M., Sahin, Y., et al. (2010) Determination of Oxidant Stress in Plasma of Rheumatoid Arthritis and Primary Osteoarthritis Patients. Indian Journal of Biochemistry & Biophysics, 47, 353-358.
[7]  Nagy, P. (2013) Kinetics and Mechanisms of Thiol-Disulfide Exchange Covering Direct Substitution and Thiol Oxidation-Mediated Pathways. Antioxidants & Redox Signaling, 18, 1623-1641.
[8]  Turell, L., Radi, R. and Alvarez, B. (2013) The Thiol Pool in Human Plasma: The Central Contribution of Albumin to Redox Processes. Free Radical Biology & Medicine, 65, 244-253.
[9]  Erel, O. and Neselioglu, S. (2014) A Novel and Automated Assay for Thiol/Disulphide Homeostasis. Clinical Biochemistry, 47, 326-332.
[10]  Ellman, G. and Lysko, H. (1979) A Precise Method for the Determination of Plasma Sulfhydryl Groups. Analytical Biochemistry, 93, 98-102.
[11]  Matteucci, E. and Giampietro, O. (2010) Thiol Signalling Network with an Eye to Diabetes. Molecules, 15, 8890-8903.
[12]  Go, Y.M. and Jones, D.P. (2011) Cysteine/Cystine Redox Signaling in Cardiovascular Disease. Free Radical Biology & Medicine, 50, 495-509.
[13]  Jonas, C.R., Ziegler, T.R. and Gu, L.H. (2002) Extracellular Thiol/Disulfide Redox State Affects Proliferation Rate in a Human Colon Carcinoma (Caco2) Cell Line. Free Radical Biology & Medicine, 33, 1499-1506.
[14]  Smeyne, M. and Smeyne, R.J. (2013) Glutathione Metabolism and Parkinson’s Disease. Free Radical Biology & Medicine, 62, 13-25.
[15]  Steele, M.L., Fuller, S. and Maczurek, A.E. (2013) Chronic Inflammation Alters Production and Release of Glutathione and Related Thiols in Human U373 Astroglial Cells. Cellular and Molecular Neurobiology, 33, 19-30.
[16]  Lluis, J.M, Morales, A. and Blasco, C. (2005) Critical Role of Mitochondrial Glutathione in the Survival of Hepatocytes during Hypoxia. The Journal of Biological Chemistry, 280, 3224-3232.
[17]  Himmelfarb, J., McMonagle, E. and McMenamin, E. (2000) Plasma Protein Thiol Oxidation and Carbonyl Formation in Chronic Renal Failure. Kidney International, 58, 2571-2578.
[18]  First, M.B. and Pincus, H.A. (2002) The DSM-IV Text Revision: Rationale and Potential Impact on Clinical Practice. Psychiatric Services, 53, 288-292.
[19]  Kuloglu, M., Atmaca, M., Tezcan, E., Ustundag, B. and Bulut, S. (2003) Antioxidant Enzyme and Malondialdehyde Levels in Patients with Panic Disorder. Neuropsychobiology, 46, 186-189.
[20]  Herken, H., Akyol, O., Yilmaz, H.R., Tutkun, H., Savas, H.A. and Ozen, M.E. (2006) Nitric Oxide, Adenosine Deaminase, Xanthine Oxidase and Superoxide Dismutase in Patients with Panic Disorder: Alterations by Antidepressan Treatment. Human Psychopharmacology, 21, 53-59.
[21]  Biswas, S., Chida, A.S. and Rahman, I. (2006) Redox Modifications of Protein Thiols: Emerging Roles in Cell Signaling. Biochemical Pharmacology, 28, 551-564.
[22]  Cremers, C.M. and Jakob, U. (2013) Oxidant Sensing by Reversible Disulfide Bond Formation. The Journal of Biological Chemistry, 288, 26489-26496.
[23]  Jones, D.P. and Liang, Y. (2009) Measuring the Poise of Thiol/Disulfide Couples in Vivo. Free Radical Biology & Medicine, 47, 1329-1338.
[24]  Rodrigues, S.D., Batista, G.B. and Ingberman, M. (2012) Plasma Cysteine/Cystine Reduction Potential Correlateswith Plasma Creatinine Levels in Chronic Kidney Disease. Blood Purification, 34, 231-237.


comments powered by Disqus

Contact Us


微信:OALib Journal