Validated procedures play an
important role to obtain accurate information about individual amino acid
requirement data. The aim of the present study was to assess lysine (Lys)
requirement of growing chicken both by classical supplementation technique and principles of diet dilution technique as
applied with “Goettingen approach”. During the starter period (1 - 21 d),
a growth study with male meat type chicken (Ross 308) was conducted making use
of five graded dietary Lys-levels (3 repetition boxes with 3 birds/box). L-Lys×HCl was gradually added to a diet based on wheat,
soybean protein concentrate, wheat gluten and fishmeal to yield 80%, 87.5%, 95%,
102.5% and 110% of the expected requirement level (13 g Lys/kg as fed). Diets
were iso-energetic (12.8 MJME/kg) and iso-nitrogenous (21.65% crude protein).
Birds were fed on free choice level also to assess the feed intake (FI) effects
as important factor on traditional response criteria. Analyzed body composition
at start and end of the growth study yielded N deposition (ND) data for further
data assessment using exponential approximations depending on dietary Lys
content or observed Lys intake. The results indicated significant differences (p < 0.05) in response on body weight
gain (BWG) and observed dietary protein quality with unexpected consequences
for the derived Lys requirement data. According to the independent variable
(Lys in % of diet versus daily Lys intake) and aimed level of daily ND, the
needed in-feed content of Lys varied between 1.24% and 1.46%. Application of
the exponential modelling by “Goettingen approach” overcame these misleading
conclusions by modelling the relationship between required Lys intake and
observed response data (BWG, ND) taking also into account the expected real feed intake to formulate the
needed in-feed concentration.
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