金针菇菇脚酸性多糖结构表征
Structural Characterization of Acid Polysaccharides from the Base of Flammulina Velutipes

DOI: 10.12677/HJFNS.2016.54018, PP. 149-156

Keywords: 金针菇菇脚，多糖，分离，纯化
The Base of Flammulina Velutipes, Polysaccharides, Isolation, Purification

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Abstract:

本文采用DEAE-52纤维素离子交换层析和Sephadex G-100层析对金针菇菇脚多糖进行分离纯化，得到两个酸性多糖组分FVP₁和FVP₂，相对分子质量分别为1.9 × 10⁶ Da和9.8 × 10³ Da。通过红外光谱鉴定，表明FVP₁和FVP₂分别为β-吡喃型半乳聚糖和α-吡喃型葡聚糖。TLC法表明FVP₁的单糖组成为半乳糖和葡萄糖，FVP₂的单糖组成为葡萄糖。
Acid polysaccharides extracted from the flammulina velutipes were isolated and purified by DEAE-52 cellulose and gel chromatography. Their preliminary structural characterizations were also analyzed. The results showed that the purified acid polysaccharides contained no protein. The molecular weights of FVP₁ and FVP₂ were 1.9 × 10⁶ Da and 9.8 × 10³ Da, respectively. By the analysis of IR, FVP₁ was a beta pyranose galactan, and FVP₂ was alpha glucan pyran type. TLC proved that FVP₁ was composed of galactose and glucose, and FVP₂ was composed of glucose.

References

[1]	傅亚欣. 金针菇菌柄基部多糖的提取纯化及细胞免疫活性研究[D]:[硕士学位论文]. 南京: 南京农业大学, 2012.
[2]	Ren, A.-N., Lu, Y., Zou, Y.-F., et al. (2013) Separation, Purification and Preliminary Structure Analysis of Acidic Polysaccharides from Safflower. European Food Research and Technology, 237, 449-455. http://dx.doi.org/10.1007/s00217-013-2009-7
[3]	李守勉, 任清, 李明, 等. 金针菇多糖的提取及其美容功效评价[J]. 食用菌, 2009, 31(5): 72-73.
[4]	占建波. 金针菇多糖提取纯化及生物活性研究[J]. 农业科技辑, 2011(S1): 109-170.
[5]	杨文建. 金针菇多糖的分离纯化, 结构分析及其记忆功能改善作用机制研究[D]: [博士学位论文]. 南京: 南京农业大学, 2012.
[6]	邓颖琳. 金针菇子实体多糖的提取分离纯化及结构鉴定[D]: [硕士学位论文]. 浙江: 浙江工业大学, 2011.
[7]	Chen, R.Z., Yang, S.M., Dong, H., et al. (2012) Polysaccharides from Acanthopanacis Senticosi Isolation Purification Antioxidant and Immunological Activities in Vitro. Chinese Academy of Sciences, 45, 955-956.
[8]	李敏晶, 毛婕昕, 付荣香, 等. DEAE-纤维素分离提纯海带硫酸多糖的研究[J]. 广东化工, 2010, 37(9): 30-31.
[9]	刘芳, 陈贵堂, 胡秋辉, 等. 金针菇锌多糖分离纯化及其结构特征[J]. 食品科学, 2014, 35(5): 1-7.
[10]	卢艳. 金针菇子实体水溶性多糖纯化结构鉴定及对乙酰胆碱酯酶抑制活性的研究[D]: [硕士学位论文]. 浙江: 浙江工业大学, 2011.
[11]	夏朝红, 戴奇, 房韦, 等. 几种多糖的红外光谱研究[J]. 武汉理工大学学报, 2007, 29(1): 45-47.
[12]	屠婕红, 陈伟光, 盛静, 等. 葎草多糖的提取分离及组成性质研究[J]. 北京中医药大学学报, 2008, 31(18): 561-563.
[13]	贾建波, 李相前, 杨文, 等. 桑黄多糖分离纯化及其结构初步鉴定[J]. 食品科学, 2006, 27(12): 446-449.
[14]	武汉大学. 分析化学[M]. 北京: 高等教育出版社, 2007: 273.
[15]	超声波辅助提取水溶性大豆多糖及纯化工艺[J]. 食品科学, 2011, 32(6): 139-143.

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