1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described here the isolation of strain ZH-1, which has the ability of high production with 1,3-propanediol, from Fenhe River in China. It was classified as a member of K. pneumoniae after the study of phenotypic, physio-logical, biochemical and phylogenetic (16S rDNA). The initial glycerol concentration, fermentation time and pH value of strain ZH-1 were determined to be 50 g·L-1, 36 h and 8.0. Under these conditions, the practical yield of 1,3-PD was 18.53 g·L-1 and a molar yield (mol1,3-PD molGlycerol-1 of 1,3-propanediol to glycerol of 0.497. In addition, we found that for the strain ZH-1, the optimum grown pH was 9.0, so we can deter-mine that it is a new member of alkali-resistant strains.
References
[1]
Zhang, G.L., Ma, B.B., Xu, X.L., Li, C. and Wang, L.W. (2007) Fast Conversion of Glycerol to 1,3-Propanediol by a New Strain of Klebsiella pneumoniae. Biochemical Engineering Journal, 37, 256-260. http://dx.doi.org/10.1016/j.bej.2007.05.003
[2]
Zheng, P., Wereath, K., Sun, J.B., Heuvel, J. and Zeng, A.P. (2006) Overexpression of Genes of the DHA Regulation and Its Effects on Cell Growth, Glycerol Fermentation to 1,3-Pro- panediol and Plasmid Stability in Klebsiella pneumoniae. Process Biochemistry, 41, 2160- 2169. http://dx.doi.org/10.1016/j.procbio.2006.06.012
[3]
Zheng, Z.M., Guo, N.N., Hao J., Cheng, K.K., Sun, Y., et al. (2009) Scale-Up of Micro- Aerobic 1,3-Propanediol Production with Klebsiella pneumoniae CGMCC 1.6366. Process Biochemistry, 44, 944-948. http://dx.doi.org/10.1016/j.procbio.2009.04.017
[4]
Barbirato, F., Himmi, E.H., Conte, T. and Bories, A. (1998) 1,3-Propanediol Production by Fermentation: An Interesting Way to Valorize Glycerin from the Ester and Ethanol Industries. Industrial Crops and Products, 7, 281-289.
http://dx.doi.org/10.1016/S0926-6690(97)00059-9
[5]
Wong, C.L., Huang, C.C., Chen, W.M. and Chang, J.S. (2011) Converting Crude Glycerol to 1,3-Propandiol Using Resting and Immobilized Klebsiella sp. HE-2 Cells. Biochemical Engineering Journal, 58-59, 177-183. http://dx.doi.org/10.1016/j.bej.2011.09.015
[6]
Biebl, H., Zeng, A.P., Menzel, K. and Deckwer, W.D. (1998) Fermentation of Glycerol to 1,3-Propanediol and 2,3-Butanediol by Klebsiella pneumoniae. Applied Microbiology and Biotechnology, 50, 24-29. http://dx.doi.org/10.1007/s002530051251
[7]
Mu, Y., Xiu, Z.L. and Zhang, D.J. (2008) A Combined Bioprocess of Biodiesel Production by Lipase with Microbial Production of 1,3-Propanediol by Klebsiella pneumoniae. Biochemical Engineering Journal, 40, 537-541. http://dx.doi.org/10.1016/j.bej.2008.02.011
[8]
Zhao, Y.N., Chen, G. and Yao, S.J. (2006) Microbial Production of 1,3-Propanediol from Glycerol by Encapsulated Klebsiella pneumoniae. Biochemical Engineering Journal, 32, 93-99. http://dx.doi.org/10.1016/j.bej.2006.09.007
[9]
Saxe, R.K., Casali, S., Gungormusler, M., Bertin, L., Fava, F., et al. (2012) Development of a Biofilm Technology for the Production of 1,3-Propanediol(1,3-PDO) from Crude Glycerol. Biochemical Engineering Journal, 64, 84-90. http://dx.doi.org/10.1016/j.bej.2011.11.012
[10]
Rodriguez, A., Wojtusik, M., Ripoll, V., Santos, V.E. and Garcia-Ochoa, F. (2016) 1,3-Pro- panediol Production from Glycerol with a Novel Biocatalyst Shimwellia blattae ATCC 33430: Operational Conditions and Kinetics in Batch Cultivations. Bioresource Technology, 200, 830-837. http://dx.doi.org/10.1016/j.biortech.2015.10.061
[11]
Silva, G.P., Lima, C.J. and Contiero, J. (2015) Production and Productivity of 1,3-Propane- diol from Glycerol by Klebsiella pneumoniae GLC29. Catalysis Today, 257, 259-266.
http://dx.doi.org/10.1016/j.cattod.2014.05.016
[12]
Reimann, A., Biebl, H. and Deckwer, W.D. (1998) Production of 1,3-Propanediol by Clostridium butyricum in Continuous Culture with Cell Recycling. Applied Microbiology and Biotechnology, 49, 359-363. http://dx.doi.org/10.1007/s002530051182
[13]
Biebl, H. (2001) Fermentation of Glycerol by Clostridium pasteurianum—Batch and Continuous Culture Studies. Journal of Industrial Microbiology and Biotechnology, 7, 18-26.
http://dx.doi.org/10.1038/sj.jim.7000155
[14]
Vivek, N., Pande, A. and Binod, P. (2016) Biological Valorization of Pure and Crude Glycerol into 1,3-Propanediol Using a Novel Isolate Lactobacillus brevis N1E9.3.3. Bioresource Technology, 213, 222-230. http://dx.doi.org/10.1016/j.biortech.2016.02.020
[15]
Wu, Z., Wang, Z., Wang, G.Q. and Tan, T.W. (2013) Improved 1,3-Propanediol Production by Engineering the 2,3-Butanediol and Formic Acid Pathways in Integrative Recombinant Klebsiella pneumoniae. Journal of Biotechnology, 168, 194-200.
http://dx.doi.org/10.1016/j.jbiotec.2013.04.022
[16]
Buchanan, R.E. (1984) Bergey’s Manual of Determinatve Bacteriology. Williams & Wilkins, Baltimore.
[17]
Liu, J., Miao, S., Wen, X. and Sun, Y. (2009) Optimization of Polysaccharides (ABP) Extraction from the Fruiting Bodies of Agaricus blazei Murill Using Response Surface Methodology (RSM). Carbohydrate Polymers, 78, 704-709.
http://dx.doi.org/10.1016/j.carbpol.2009.06.003
[18]
Wang, J.F., Xiu, Z.L. and Fan, S.D. (2001) Determination of Glycerin Concentration during the Fermentation of Glycerin to 1,3-Propanediol. Industrial Microbiology, 31, 33-35.
[19]
Jun, S.A., Moon, C., Kang, C.H., Kong, S.W., Sang, B.I., et al. (2010) Microbial Fed-Batch Production of 1,3-Propanediol Using Raw Glycerol with Suspended and Immobilized Klebsiella pneumoniae. Applied Biochemistry and Biotechnology, 161, 491-501.
http://dx.doi.org/10.1007/s12010-009-8839-x
[20]
Saxena, R.K., Anand, P., Saran, S. and Isar, J. (2009) Microbial Production of 1,3-Propane- diol: Recent Developments and Emerging Opportunities. Biotechnology Advances, 27, 895- 913. http://dx.doi.org/10.1016/j.biotechadv.2009.07.003
[21]
Cheng, K.K., Zhang, J.A. and Liu, D.H. (2006) Production of 1,3-Propanediol by Klebsiella pneumoniae from Glycerol Broth. Biotechnology Letters, 28, 1817-1821.
http://dx.doi.org/10.1007/s10529-006-9158-8
[22]
Chen, X., Zhang, D.J., Qi, W.T., Gao, S.J., Xiu, Z.L., et al. (2003) Microbial Fed-Batch Production of 1,3-Propanediol by Klebsiella pneumoniae under Micro-Aerobic Conditions. Applied Microbiology and Biotechnology, 63, 143-146.
http://dx.doi.org/10.1007/s00253-003-1369-5
[23]
Zheng, Z.M., Hu, Q.L., Hao, J., Xu, F., Guo, N.N., et al. (2008) Statistical Optimization of Culture Conditions for 1,3-Propanediol by Klebsiella pneumoniae AC 15 via Central Composite Design. Bioresource Technology, 99, 1052-1056.
http://dx.doi.org/10.1016/j.biortech.2007.02.038
[24]
Yang, G., Tian, J.S. and Li, J.L. (2007) Fermentation of 1,3-Propanediol by a Lactate Deficient Mutant of Klebsiella oxytoca under Microaerobic Conditions. Applied Microbiology and Biotechnology, 73, 1017-1024. http://dx.doi.org/10.1007/s00253-006-0563-7
