Our aim was to study the selected cases of the patients with ischemic heart disease and to analyze the structure of blood serum of patients in comparison with control serum of healthy subjects by methods: synchronous fluorescence fingerprint and atomic force microscopy that are still not used in clinical practice. Our results of fluorescence analysis showed that blood serum of all patients with ischemic heart disease decreased intensity of fluorescence in comparison with control blood serum. Endogenous fluorescence of synchronous fluorescence fingerprint of blood serum of patients with unstable angina pectoris state after non ST elevation myocardial infarction; angina pectoris and arterial hypertension 3 was similar, but atomic force microscopy revealed differences in the structure of blood serum of patients with the angina pectoris. Blood serum of patients with angina pectoris exhibited disappearance of fluorescence peak with maximum fluorescence and showed lower fluorescence intensity than control blood serum and blood serum of patients with arterial hypertension 2. Profiles of synchronous fluorescence fingerprint of blood serum of patients with arterial hypertension stage 2 showed formation of the new fluorescent peak with maximum fluorescence, similar shape of synchronous fluorescence fingerprint and higher fluorescence intensity than blood serum of healthy subjects. Blood serum sensitively revealed changes in the body by using untraditional novel techniques which enable the analysis of the mixture of blood serum and might be a new possibility in study of heart ischemia diseases.
O’Gara, P.T., et al. (2013) 2013 ACCF/AHA Guideline for the Management of ST-Elevation Myocardial Infarction: A Report of the American College of Cardiology Foundation/ American Heart Association Task Force on Practice Guidelines. Circulation, 127, 362-425.
http://dx.doi.org/10.1161/CIR.0b013e3182742c84
[3]
Opie, L.H. (1975) Metabolism of Free Fatty Acids, Glucose and Catecholamines in Acute Myocardial Infarction: Relation to Myocardial Ischemia and Infarct Size. The American Journal of Cardiology, 36, 938-953. http://dx.doi.org/10.1016/0002-9149(75)90086-7
[4]
Bodi, V., et al. (2012) Metabolomic Profile of Human Myocardial Ischemia by Nuclear Magnetic Resonance Spectroscopy of Peripheral Blood Serum: A Translational Study Based on Transient Coronary Occlusion Models. Journal of the American College of Cardiology, 59, 1629-1641. http://dx.doi.org/10.1016/j.jacc.2011.09.083
[5]
Flierl, M.A., Rittirsch, D., Huber-Lang, M., Sarma, J.V. and Ward, P.A. (2008) Catecholamines-Crafty Weapons in the Inflammatory Arsenal of Immune/Inflammatory Cells or Opening Pandora’s Box? Molecular Medicine, 14, 195.
[6]
Wannemacher, R.W., Klainer, A.S., Dinterman, R.E. and Beisel, W.R. (1976) The Significance and Mechanism of an Increased Serum Phenylalanine-Tyrosine Ratio during Infection. The American Journal of Clinical Nutrition, 29, 997-1006.
[7]
Guleria, A., et al. (2015) NMR-Based Serum Metabolomics Discriminates Takayasu Arteritis from Healthy Individuals: A Proof-of-Principle Study. Journal of Proteome Research, 14, 3372. http://dx.doi.org/10.1021/acs.jproteome.5b00422
[8]
Sun, L., et al. (2012) Metabonomics Reveals Plasma Metabolic Changes and Inflammatory Marker in Polycystic Ovary Syndrome Patients. Journal of proteome research, 11, 2937- 2946. http://dx.doi.org/10.1021/pr3000317
[9]
Deidda, M., et al. (2015) Metabolomic Approach to Profile Functional and Metabolic Changes in Heart Failure. Journal of Translational Medicine, 13, 297.
http://dx.doi.org/10.1186/s12967-015-0661-3
[10]
Zhong, X., et al. (2012) Glycine Attenuates Myocardial Ischemia-Coreperfusion Injury by Inhibiting Myocardial Apoptosis in Rats. Journal of Biomedical Research, 26, 346-354.
http://dx.doi.org/10.7555/JBR.26.20110124
[11]
Drake, K.J., Sidorov, V.Y., McGuinness, O.P., Wasserman, D.H. and Wikswo, J.P. (2012) Amino Acids as Metabolic Substrates during Cardiac Ischemia. Experimental Biology and Medicine, 237, 1369-1378. http://dx.doi.org/10.1258/ebm.2012.012025
[12]
Ouyang, X., Dai, Y., Wen, J.L. and Wang, L.X. (2011) (1)H NMR-Based Metabolomic Study of Metabolic Profiling for Systemic Lupus Erythematosus. Lupus, 20, 1411.
http://dx.doi.org/10.1177/0961203311418707
[13]
Filippo, C.D., Cuzzocrea, S., Rossi, F., Marfella, R. and D’Amico, M. (2006) Oxidative Stress as the Leading Cause of Acute Myocardial Infarction in Diabetics. Cardiovascular Drug Reviews, 24, 77-87. http://dx.doi.org/10.1111/j.1527-3466.2006.00077.x
[14]
Keller, J.N. (2006) Interplay between Oxidative Damage, Protein Synthesis, and Protein Degradation in Alzheimer’s Disease. BioMed Research International, 2006, 12129.
[15]
Berezin, M.Y. and Achilefu, S. (2010) Fluorescence Lifetime Measurements and Biological Imaging. Chemical Reviews, 110, 2641-2684. http://dx.doi.org/10.1021/cr900343z
[16]
Tomecková, V., Bohó, A., Komanicky V., Tomecko, M. and Gu1'asová, Z. (2015) Monitorovanie rôznych srdcovych ochorení z autofluorescencie krvi. Ateroskleróza, 19, 694.
[17]
Zhang X. Fales A. and Vo-Dinh, T. (2015) Time-Resolved Synchronous Fluorescence for Biomedical Diagnosis. Sensors, 15, 21746-21759. http://dx.doi.org/10.3390/s150921746
[18]
Bohó, A., Tomecková, V. and Sedláková, E. (2012) Vyuzitie fluorescencnej analyzy pri vcasnej diagnostike akútneho koronárneho syndrómu. Cardiology Letters, 21, 124-130.
[19]
Gu1'asová, Z., et al. (2015) Monitoring of Thoracic Aortic Aneurysm in Blood by Fluorescence Spectroscopy. American Journal of Medical and Biological Research, 3, 128-132.
http://dx.doi.org/10.12691/ajmbr-3-5-2
[20]
Zhu, W., Zhao, Z., Guo, X. and Hong, X. (2010) Study on Serum Fluorescence Spectra Based on Wavelet Transform. African Journal of Biotechnology, 9, 892-899.
http://dx.doi.org/10.5897/AJB09.1412
[21]
Masilamani, V., et al. (2012) Fluorescence Spectra of Blood and Urine for Cervical Cancer Detection. Journal of Biomedical Optics, 17, 098001.
http://dx.doi.org/10.1117/1.jbo.17.9.098001
