Hemorrhage during trauma occurred in emergency situations is a significant challenge.
It may be life threatening if it is not treated swiftly. A new device which can
effectively stop bleeding to save life of injured person, especially in battlefield situations
and accidents, is presented. A plasma generator is designed to generate a low
temperature air plasma spray for treating wounds. The spectral spike at 777.4 nm in
the emission spectrum of the plasma plume and the spatial distribution of this emission
line’s spectral intensity indicate that abundant atomic oxygen is generated and
sprays out of the generator by about 25 mm. Atomic oxygen carried by the plasma
spray can quickly activate the cascading of coagulation processes and works as dry
disinfectant to advance healing. Tests on blood droplets reveal the strong dependence
of blood clotting on the amount of atomic oxygen applied in the plasma treatment,
which is maneuvered by increasing the plasma treatment time or decreasing the exposure
distance; in both approaches, the degree of blood clotting increases. Treated
smeared blood samples show that an increase of the erythrocyte concentration and a
drastic decrease of the platelet count are also correlated to the increase of atomic
oxygen dose applied in the plasma treatment. The results reveal the mechanisms of
air plasma blood coagulation and wound healing. As animal models, pigs were used
in the tests of stopping wound bleeding from a cross cut in the ham area, from a hole
in an ear’s saphenous vein, and from cuts to arteries in an ear and in a real leg, all
stopped swiftly. Moreover, both artery cuts were secure to remove tourniquet; downgrade
of tourniquet necessary wound in under 2 minutes was demonstrated. The
healing progress of cross cut wounds was observed. The healing time was shortened
to about half. This battery power plasma spray can be carried to or placed at anywhere
available for first aid applications. It stops bleeding swiftly to save life, and also
downgrades tourniquet necessary wound to extend the golden period of saving the
remaining part below tourniquet.
References
[1]
Jevon, P. and Cooper, L. (2005) First Aid. Part 5. First-Aid Treatment for Severe Bleeding. Nursing Times, 104, 26-27.
[2]
USA Today (2009) Advanced First Aid for Troops Sought. A1, 14 September.
[3]
Spinella, P.C., Perkins, J.G., McLaughlin, D.F., Niles, S.E., Grathwohl, K.W., Beekley, A.C., Salinas, J., Mehta, S., Wade, C.E. and Holcomb, J.B. (2008) The Effect of Recombinant Activated Factor VII on Mortality in Combat-Related Casualties with Severe Trauma and Massive Transfusion. Journal of Trauma, 64, 286-294.
http://dx.doi.org/10.1097/TA.0b013e318162759f
[4]
Kramer, A.H., Diringer, M.N., Suarez, J.I., Naidech, A.M., Macdonald, L.R. and Le Roux, P.D. (2011) Red Blood Cell Transfusion in Patients with Subarachnoid Hemorrhage: A Multidisciplinary North American Survey. Critical Care, 15, R30.
http://dx.doi.org/10.1186/cc9977
Kuo, S.P. (2012) Air Plasma for Medical Applications. Journal of Biomedical Science & Engineering, 5, 481-495. http://dx.doi.org/10.4236/jbise.2012.59061
[13]
Chen, C.Y., Fan, H.W., Kuo, S.P., Chang, J., Pedersen, T., Mills, T. and Huang, C.C. (2009) Blood Clotting by Low Temperature Air Plasma. IEEE Transaction on Plasma Science, 37, 993-999. http://dx.doi.org/10.1109/TPS.2009.2016344
[14]
Kuo, S.P., Tarasenko, O., Chang, J., Popovic, S., Chen, C., Fan, H., Scott, A., Lahiani, M., Alusta, P., Drake, J. and Nikolic, M. (2009) Contribution of a Portable Air Plasma Torch to Rapid Blood Coagulation as a Method of Preventing Bleeding. New Journal of Physics, 11, Article ID: 115016. http://dx.doi.org/10.1088/1367-2630/11/11/115016
[15]
Kuo, S.P., Chen, C.Y., Lin, C.S. and Chiang, S.H. (2010) Wound Bleeding Control by Low Temperature Air Plasma. IEEE Transaction on Plasma Science, 38, 1908-1914.
http://dx.doi.org/10.1109/TPS.2010.2047028
[16]
Kuo, S.P., Chen, C.Y., Lin, C.S. and Chiang, S.H. (2012) Applications of Air Plasma for Wound Bleeding Control and Healing. IEEE Transactions on Plasma Science, 40, 1117- 1123. http://dx.doi.org/10.1109/TPS.2012.2184142
[17]
Kuo, S.P. (2015) Battery Powered Handheld Air Plasma Spray. US Patent No. 8927896B2.
[18]
Kuo, S.P., Pedersen, T. and Mills, T. (2008) Lateral Distribution of Atomic Oxygen Flux Produced by an Array of Three Fan-Shaped Plasma Torches. IEEE Transactions on Plasma Science, 36, 1056-1057. http://dx.doi.org/10.1109/TPS.2004.924556
[19]
Kuo, S.P., Pedersen, T. and Mills, T. (2011) Two-Dimensional Distribution of Atomic Oxygen Multiplet Radiation Produced by an Air Plasma Torch. IEEE Transactions on Plasma Science, 39, 2282-2283. http://dx.doi.org/10.1109/TPS.2011.2155089
[20]
O’Connor, N., Humphreys, H.H. and Daniels, S. (2013) Oxygen Line Ratio Method for the Determination of Plasma Parameters in Atmospheric Pressure Discharges Using Air as the Working Gas. 31st International Conference on Phenomena in Ionized Gases (ICPIG), Granada, 14-19 July 2013.
[21]
Anthea, M., Hopkins, J., Johnson, S., McLaughlin, D.L.C., Warner, M.Q. and Wright, J.D. (1994) Human Biology and Health. Prentice Hall, Englewood Cliffs.
[22]
Reimers, R.C., Sutera, S.P. and Joist, H.J. (1984) Potentiation by Red Blood Cells of Shear- Induced Platelet Aggregation: Relative Importance of Chemical and Physical Mechanisms. Blood, 64, 1200-1206.
[23]
Sachs, U.J. and Nieswandt, B. (2007) In Vivo Thrombus Formation in Murine Models. Circulation Research, 100, 979-991. http://dx.doi.org/10.1161/01.RES.0000261936.85776.5f
[24]
Kolev, K., Longstaff, C. and Machovich, R. (2005) Fibrinolysis at the Fluid-Solid Interface of Thrombi. Current Medicinal Chemistry: Cardiovascular & Hematological Agents, 3, 341- 355. http://dx.doi.org/10.2174/156801605774322337
[25]
Bethesda, L.D. (2005) Blood Groups and Red Cell Antigens. National Library of Medicine (US): NCBI.
[26]
http://www.genomesize.com/cellsize/mammals.htm
[27]
Greer, J.P., Foerster, J., Rodgers, G.M., Paraskevas, F., Glader, B., Arber, D.A. and Means Jr., R.T. (2003) Wintrobe’s Clinical Hematology. 11th Edition, Lippincott Williams & Wilkins, Philadelphia.
[28]
Fridman, G., Shereshevsky, A., Jost, M., Brooks, A., Fridman, A., Gutsol, A., Vasilets, C.V. and Friedman, G. (2007) Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines. Plasma Chemistry & Plasma Processing, 27, 163-176. http://dx.doi.org/10.1007/s11090-007-9048-4
[29]
Machovich, R. and Owen, W.G. (1990) The Elastase-Mediated Pathway of Fibrinolysis. Blood Coagulation & Fibrinolysis, 1, 79-90.
http://dx.doi.org/10.1097/00001721-199003000-00011
[30]
Born, G.V.R., Bergquist, D. and Arfors, K.E. (1976) Evidence for Inhibition of Platelet Activation in Blood by a Drug Effect on Erythrocytes. Nature, 259, 233-235.
http://dx.doi.org/10.1038/259233a0
[31]
Bergquist, D. and Arfors, K.E. (1980) Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation—An Analysis of Red Blood Cell Participation. Thrombosis and Haemostasis, 44, 6-8.
[32]
Schmid-Schdnbein, H. and Teitel, P. (Eds.) (1979) Basic Aspects of Blood Trauma. Schattauer Verlag, Stuttgart, 322-340. http://dx.doi.org/10.1007/978-94-009-9337-2_15
[33]
Tiefenbach, H.J., Durchschlag, H., Schneider, G. and Jaenicke, R. (2004) Thermodynamic Analysis of Serum Albumin Denaturation by Sodium Dodecyl Sulfate. Aqueous Polymer Dispersions, 124, 130-140.
[34]
Schmid-Schdnbein, H., Born, G.V.R., Richardson, P.D., Cusack, N., Rieger, H., Forst, R., Rohling-Winkel, J., Blasberg, P. and Wehmeyer, A. (1981) Rheology of Thrombotic Processes in Flow: The Interaction of Erythrocytes and Thrombocytes Subjected to High Flow Forces. Biorheology, 18, 415-444.
[35]
Massberg, S., Grahl, L., von Bruehl, M., Manukyan, D., et al. (2010) Reciprocal Coupling of Coagulation and Innate Immunity via Neutrophil Serine Proteases. Nature Medicine, 16, 887-896. http://dx.doi.org/10.1038/nm.2184
[36]
Kolev, K. and Colin, L. (2016) Bleeding Related to Disturbed Fibrinolysis. British Journal of Haematology, 1-12. http://dx.doi.org/10.1111/bjh.14255
[37]
Meireles, M., Aimar, P. and Sanchez, V. (2004) Albumin Denaturation during Ultrafiltration: Effects of Operating Conditions and Consequences on Membrane Fouling. Biotechnology and Bioengineering, 38, 528-534. http://dx.doi.org/10.1002/bit.260380511
[38]
Davis, J.C. and Hunt, T.K. (1988) Problem Wounds: The Role of Oxygen. Elsevier, Berlin.
[39]
La Van, F.B. and Hunt, T.K. (1990) Oxygen and Wound Healing. Clinics in Plastic Surgery, 17, 463-472.
[40]
Harman, D. (1956) Aging: A Theory Based on Free Radical and Radiation Chemistry. Journal of Gerontology, 11, 298-300. http://dx.doi.org/10.1093/geronj/11.3.298