Antibacterial Activity of Moringa ovalifolia and Moringa oleifera Methanol, N-Hexane and Water Seeds and Bark Extracts against Pathogens That Are Implicated in Water Borne Diseases
Antibacterial
activity of methanol, n-hexane and water extracts of seeds and bark of Moringa
oleifera and Moringa ovalifolia was conducted. The causative agents
of common bacterial infections that are thought to cause water-borne diseases,
namely, Escherichia coli, Enterococcus faecalis, and Bacillus
cereus were chosen for the study. The paper-disc diffusion
method was used with treatments arranged in a completely randomized design and
replicated four times. The M. oleifera extracts
showed more inhibitory effect than those of M.
ovolifolia. The conventional antibiotic Ampicilin generally showed higher
inhibitory effect than the extracts of both M.
oleifera and M. ovalifolia. The
inhibitory effect varied depending on the solvent used. The n-hexane extracts
of both seeds and bark of M. ovalifolia and M. oleifera had almost the same
inhibition activities (6 ± 1 mm mean inhibition zones) on B. cereus, E. coli
References
[1]
Al-Bari, M.A., Sayeed, M.A., Bahman, M.S. and Mossadik, M.A. (2006) Characterization and Antimicrobial Activities of a Phenolic Acid Derivative Produced by Streptomyces bangladeshiensis, a Novel Species Collected in Bangladesh. Research Journal of Medicine and Medical Sciences, 1, 77-81.
[2]
Bekele, A.B., Abera, R., Mebratu, T., Dessie, W., Getu, A. and Getnet, B. (2015) Antimicrobial Activity of Thymus schimperi Ronninger (Lamiaceae) against Standard and Clinical Isolates of Human Pathogenic Bacteria. Journal of Medicinal Plants Research, 4, 379-385.
[3]
Muyibi, S.A. and Evison, L.M. (1995a) Moringaoleifera Seeds for Softening Hard Water. Water Research, 29, 1099-1105. http://dx.doi.org/10.1016/0043-1354(94)00250-B
[4]
Danazumi, S. and Bichi, M.H. (2010b) Industrial Pollution and Implication on Source of Water Supply in Kano, Nigeria. International Journal of Engineering & Technology IJET-IJENS, 10, 101-110.
[5]
Bichi, M.H. (2013) A Review of the Applications of Moringaoleifera Seeds Extract in Water Treatment. Civil and Environmental Research, 3, 1-9.
[6]
Arbuckle, T.E., Hrudey, S.E., Krasner, S.W., Nuckols, J.R., Richardson, S.D., Singer, P., Mendola, P., Dodds, L., Weisel, C., Ashley, D.L., Froese, K.L., Pegram, R.A., Schultz, I.R., Reif, J., Bachand, A.M., Benoit, F.M., Lynberg, M.I., Poole, C. and Waller, K. (2002) Assessing Exposure in Epidemiologic Studies to Disinfection By-Products in Drinking Water: Report from an International Workshop. Environmental Health Perspectives, 110, 53-60. http://dx.doi.org/10.1289/ehp.02110s153
[7]
Goveas, S.W. and Abraham, A. (2013) Evaluation of Antimicrobial and Antioxidant Activity of Stem and Leaf Extracts of Coscinium fenestratum. Asian Journal of Pharmaceutical and Clinical Research, 6, 218-221.
[8]
Ouma, Y., Sharby, A. and Tateishi, R. (2005) Dynamism and Abundance of Water Hyacinth in the Winam Gulf of Lake Victoria: Evidence from Sensing and Seasonal-Climate Data. International Journal of Environmental Studies, 62, 449-465. http://dx.doi.org/10.1080/00207230500197011
[9]
Alikwe, P.C.N., Ohimain, E.J., Zige, D.V. and Angaye, T.N.C. (2013) Antibacterial Activity of Ethanol Extract of the Defatted Seed and Seed Coat of Moringaoleifera. Journal of Pharmacy and Biological Sciences, 8, 38-41. http://dx.doi.org/10.9790/3008-0813841
[10]
Silver, L.L. (1993) Discovery and Development of New Antibiotics: The Problem of Antibiotics Resistance. Antimicrobial Agents and Chemotherapy, 37, 377-383. http://dx.doi.org/10.1128/AAC.37.3.377
[11]
Doughari, J.H., El-Mahmood, A.M. and Manzara, S. (2007) Studies on the Antibacterial Activity of Root Extracts of Carica papaya L. African Journal of Microbiology Research, 1, 37-41.
[12]
Sharif, M.D.M. and Banik, G.R. (2006) Status and Utilization of Medicinal Plants in Rangamati of Bangladesh. Research Journal of Agriculture and Biological Sciences, 2, 268-273.
[13]
Zy, E.A. (2005) Antimicrobial Activity of Some Medicinal Plants Extracts in Palestine. Pakistan Journal of Medical Sciences, 2, 187-193.
[14]
Bugno, A., Nicoletti, M.A., Almodóvar, A.A.B., Pereira, T.C. and Auricchio, M.T. (2007) Antimicrobial Efficacy of Curcuma zedoaria Extracts as Assessed by Linear Regression Compared with Commercial Mouth Rinses. Brazilian Journal of Microbiology, 38, 440-445. http://dx.doi.org/10.1590/S1517-83822007000300011
[15]
Ramachandran, C., Peter, K.V. and Gopalakrishnan, P.K. (1980) Drumstick (Moringa oleifera): A Multipurpose Indian Vegetable. Economic Botany, 34, 276-283. http://dx.doi.org/10.1007/BF02858648
[16]
Fahey, J.W. (2005) Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Trees for Life Journal, 1, 5.
[17]
Kwaambwa, H.M., Hellsing, M. and Rennie, A.R. (2010) Adsorption of a Water Treatment Protein from Moringa oleifera Seeds to a Silicon Oxide Surface Studies by Neutron Reflection. Langmuir, 26, 3902-3910. http://dx.doi.org/10.1021/la9031046
[18]
Walter, A., Samuel, W., Peter, A. and Joseph, O. (2011) Antibacterial Activity of Moringa oleifera and Moringa stenopetala Methanol and n-Hexane Seed Extracts on Bacteria Implicated in Water Borne Diseases. African Journal of Microbiology Research, 5, 153-157.
[19]
Onuoha, S.C. and Alisa, C.O. (2013) Antimicrobial Potential of Leaf Juice and Extracts of Moringa oleifera Lam against Some Human Pathogenic Bacteria. IOSR Journal of Pharmacy and Biological Sciences, 5, 37-42. http://dx.doi.org/10.9790/3008-0543742
[20]
Kwaambwa, H.M., Chimuka, L., Kandawa-Schulz, M., Munkombwe, N.M. and Thwala, J.M. (2012) Situational Analysis and Promotion of the Cultivation and Utilization of the Moringa oleifera Tree in Selected Sub-Sahara Africa Countries. Progress Multidisciplinary Research Journal, 4, 9-40.
[21]
Barry, A.L. and Brown, S.D. (1996) Fluconazole Disk Diffusion Procedure for Determining Susceptibility of Candida Species. Journal of Clinical Microbiology, 34, 2154-2157.
[22]
Akinyemi, K.O., Oluwa, O.K. and Omomigbehin, E.O. (2006) Antimicrobial Activity of Crude Extracts of Three Medicinal Plants Used in South-West Nigerian Folk Medicine on Some Food Borne Bacterial Pathogens. African Journal of Traditional, Complementary, and Alternative Medicines, 3, 13-22. http://dx.doi.org/10.4314/ajtcam.v3i4.31173