Menstrual blood stem cells (MensSCs) have enormous potential as a source for cell replacement therapies. Since there is a major concern in utilization of nanofibers in tissue engineering of stem cells, we examined the potential of MensSCs to differentiate into hepatocytes, using different protocols and compare cells, with two-dimensional (2D) and three-dimensional (3D) culture systems. Cell characterization experiments of MensSCs have demonstrated that they are multipotent stem cells similar to mesenchymal stem cells, which can successfully differentiate into osteogenic and adipogenic lineages. The efficiency of the cells on the scaffold was appraised by scanning electron microscopy (SEM), MTT assay, and hematoxylin and eosin (H&E) staining. Thereafter, the differentiation protocols were developed by hepatocyte growth factor (HGF) and oncostatin M (OSM) with serum-supplemented or serum-free culture media up to 30 days. Immunofluorescence analysis and ELISA assay revealed the expression of albumin (ALB) in differentiated cells. Hepatocyte-like cells expressed liver-specific gene such as albumin(ALB), α-fetoprotein (AFP), tyrosine aminotransferase (TAT) and cytochrome P450 subunit 7a1 (Cyp7a1) at mRNA levels. In conclusion, the evidences presented in this study show that the nanofiber scaffold and MensSCs may provide a source of differentiated cells for treatment of liver diseases.
References
[1]
Haridass, D., Narain, N. and Ott, M. (2008) Hepatocyte Transplantation: Waiting for Stem Cells. Current Opinion in Organ Transplantation, 13, 627-632. http://dx.doi.org/10.1097/MOT.0b013e328317a44f
[2]
Kim, S.J., Kim, M.R., Oh, J.S., Han, I. and Shin, S.W. (2009) Effects of Polycaprolactone-Tricalcium Phosphate, Recombinant Human Bone Morphogenetic Protein-2 and Dog Mesenchymal Stem Cells on Bone Formation: Pilot Study in Dogs. Yonsei Medical Journal, 50, 825-831. http://dx.doi.org/10.3349/ymj.2009.50.6.825
[3]
Yilgor, P., Sousa, R.A., Reis, R.L., Hasirci, N. and Hasirci, V. (2008) 3D Plotted PCL Scaffolds for Stem Cell Based Bone Tissue Engineering. Macromolecular Symposia, 269, 92-99. http://dx.doi.org/10.1002/masy.200850911
[4]
Kalervo Vaananen, H. (2005) Mesenchymal Stem Cells. Annals of Medicine, 37, 469-479. http://dx.doi.org/10.1080/07853890500371957
[5]
Toma, C., Pittenger, M.F., Cahill, K.S., Byrne, B.J. and Kessler, P.D. (2002) Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine Heart. Circulation, 105, 93-98. http://dx.doi.org/10.1161/hc0102.101442
[6]
Zurita, M. and Vaquero, J. (2004) Functional Recovery in Chronic Paraplegia after Bone Marrow Stromal Cells Transplantation. Neuroreport, 15, 1105-1108. http://dx.doi.org/10.1097/00001756-200405190-00004
[7]
Vilquin, J. and Rosset, P. (2010) Mesenchymal Stem Cells in Bone and Cartilage Repair: Current Status. Regenerative Medicine, 1, 589-604. http://dx.doi.org/10.2217/17460751.1.4.589
[8]
Bentz, E.K., Kenning, M., Schneeberger, C., Kolbus, A., Huber, J.C., Hefler, L.A. and Tempfer, C.B. (2010) OCT-4 Expression in Follicular and Luteal Phase Endometrium: A Pilot Study. Reproductive Biology and Endocrinology, 8, 38. http://dx.doi.org/10.1186/1477-7827-8-38
[9]
De Coppi, P., Bartsch, G., Siddiqui, M.M., Xu, T., Santos, C.C., Perin, L. and Atala, A. (2007) Isolation of Amniotic Stem Cell Lines with Potential for Therapy. Nature Biotechnology, 25, 100-106. http://dx.doi.org/10.1038/nbt1274
[10]
Borlongan, C.V., Kaneko, Y., Maki, M., Yu, S.J., Ali, M., Allickson, J.G. and Sanberg, P.R. (2010) Menstrual Blood Cells Display Stem Cell-Like Phenotypic Markers and Exert Neuroprotection Following Transplantation in Experimental Stroke. Stem Cells and Development, 19, 439-452. http://dx.doi.org/10.1089/scd.2009.0340
[11]
Lin, J., Xiang, D., Zhang, J.L., Allickson, J. and Xiang, C. (2011) Plasticity of Human Menstrual Blood Stem Cells Derived from the Endometrium. Journal of Zhejiang University Science B, 12, 372-380. http://dx.doi.org/10.1631/jzus.B1100015
[12]
Cho, S.W., Kim, I.K., Lim, S.H., Kim, D.I., Kang, S.W., Kim, S.H. and Kim, B.S. (2004) Smooth Muscle-Like Tissues Engineered with Bone Marrow Stromal Cells. Biomaterials, 25, 2979-2986. http://dx.doi.org/10.1016/j.biomaterials.2003.09.068
[13]
Mosmann, T. (1983) Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal of Immunological Methods, 65, 55-63. http://dx.doi.org/10.1016/0022-1759(83)90303-4
[14]
Patel, A.N., Park, E., Kuzman, M., Benetti, F., Silva, F.J. and Allickson, J.G. (2008) Multipotent Menstrual Blood Stromal Stem Cells: Isolation, Characterization, and Differentiation. Cell Transplantation, 17, 303-311. http://dx.doi.org/10.3727/096368908784153922
[15]
Patel, A.N. and Silva, F. (2008) Menstrual Blood Stromal Cells: The Potential for Regenerative Medicine. Regenerative Medicine, 3, 443-444. http://dx.doi.org/10.2217/17460751.3.4.443
[16]
Van Phuc, P., Lam, D.H., Ngoc, V.B., Thu, D.T., Nguyet, N.T.M. and Ngoc, P.K. (2011) Production of Functional Dendritic Cells from Menstrual Blood—A New Dendritic Cell Source for Immune Therapy. In Vitro Cellular & Developmental Biology-Animal, 47, 368-375. http://dx.doi.org/10.1007/s11626-011-9399-2
[17]
Allickson, J.G., Sanchez, A., Yefimenko, N., Borlongan, C.V. and Sanberg, P.R. (2011) Recent Studies Assessing the Proliferative Capability of a Novel Adult Stem Cell Identified in Menstrual Blood. The Open Stem Cell Journal, 3, 4. http://dx.doi.org/10.2174/1876893801103010004
[18]
Allickson, J. and Xiang, C. (2012) Human Adult Stem Cells from Menstrual Blood and Endometrial Tissue. Journal of Zhejiang University-Science B, 13, 419-420. http://dx.doi.org/10.1631/jzus.B1200062
[19]
Glicklis, R., Shapiro, L., Agbaria, R., Merchuk, J.C. and Cohen, S. (2000) Hepatocyte Behavior within Three-Dimensional Porous Alginate Scaffolds. Biotechnology and Bioengineering, 67, 344-353. http://dx.doi.org/10.1002/(SICI)1097-0290(20000205)67:3<344::AID-BIT11>3.0.CO;2-2
[20]
Baharvand, H., Hashemi, S.M., Ashtiani, S.K. and Farrokhi, A. (2006) Differentiation of Human Embryonic Stem Cells into Hepatocytes in 2D and 3D Culture Systems in Vitro. International Journal of Developmental Biology, 50, 645. http://dx.doi.org/10.1387/ijdb.052072hb
[21]
Ong, S.Y., Dai, H. and Leong, K.W. (2006) Inducing Hepatic Differentiation of Human Mesenchymal Stem Cells in Pellet Culture. Biomaterials, 27, 4087-4097. http://dx.doi.org/10.1016/j.biomaterials.2006.03.022
[22]
Yoshimoto, H., Shin, Y.M., Terai, H. and Vacanti, J.P. (2003) A Biodegradable Nanofiber Scaffold by Electrospinning and Its Potential for Bone Tissue Engineering. Biomaterials, 24, 2077-2082. http://dx.doi.org/10.1016/S0142-9612(02)00635-X
[23]
Guex, A.G., Romano, F., Marcu, I.C., Tevaearai, H.T., Ullrich, N.D. and Giraud, M.N. (2012) Culture of Cardiogenic Stem Cells on PCL-Scaffolds: Towards the Creation of Beating Tissue Constructs. IASTED Conference Proceedings, Vol. 791. http://dx.doi.org/10.2316/P.2013.791-080
[24]
Hoveizi, E., Khodadadi, S., Tavakol, S., Karima, O. and Nasiri-Khalili, M.A. (2014) Small Molecules Differentiate Definitive Endoderm from Human Induced Pluripotent Stem Cells on PCL Scaffold. Applied Biochemistry and Biotechnology, 173, 1727-1736. http://dx.doi.org/10.1007/s12010-014-0960-9
[25]
Haynesworth, S.E., Barer, M.A. and Caplan, A.I. (1992) Cell Surface Antigens on Human Marrow-Derived Mesenchymal Cells Are Detected by Monoclonal Antibodies. Bone, 13, 69-80. http://dx.doi.org/10.1016/8756-3282(92)90363-2
[26]
Gillette, B.M., Rossen, N.S., Das, N., Leong, D., Wang, M., Dugar, A. and Sia, S.K. (2011) Engineering Extracellular Matrix Structure in 3D Multiphase Tissues. Biomaterials, 32, 8067-8076. http://dx.doi.org/10.1016/j.biomaterials.2011.05.043
[27]
Rim, N.G., Shin, C.S. and Shin, H. (2013) Current Approaches to Electrospun Nanofibers for Tissue Engineering. Biomedical Materials, 8, Article ID: 014102. http://dx.doi.org/10.1088/1748-6041/8/1/014102
[28]
Snykers, S., Vanhaecke, T., De Becker, A., Papeleu, P., Vinken, M., Van Riet, I. and Rogiers, V. (2007) Chromatin Remodeling Agent Trichostatin A: A Key-Factor in the Hepatic Differentiation of Human Mesenchymal Stem Cells Derived of Adult Bone Marrow. BMC Developmental Biology, 7, 24. http://dx.doi.org/10.1186/1471-213X-7-24
[29]
Sancho-Bru, P., Roelandt, P., Narain, N., Pauwelyn, K., Notelaers, T., Shimizu, T. and Verfaillie, C. (2011) Directed Differentiation of Murine-Induced Pluripotent Stem Cells to Functional Hepatocyte-Like Cells. Journal of Hepatology, 54, 98-107. http://dx.doi.org/10.1016/j.jhep.2010.06.014
[30]
Cai, J., Zhao, Y., Liu, Y., Ye, F., Song, Z., Qin, H., et al. (2004) Directed Differentiation of Human Embryonic Stem Cells into Functional Hepatic Cells. Hepatology, 45, 1229-1239. http://dx.doi.org/10.1002/hep.21582
[31]
Basma, H., Soto-Gutiérrez, A., Yannam, G.R., Liu, L., Ito, R., Yamamoto, T. and Fox, I.J. (2009) Differentiation and Transplantation of Human Embryonic Stem Cell-Derived Hepatocytes. Gastroenterology, 136, 990-999. http://dx.doi.org/10.1053/j.gastro.2008.10.047
