|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
抗纤软肝冲剂对肝星状细胞自分泌 转化生长因子β1的影响
Keywords: gene expression, transforming growth factorβ1, hepatic cirrhosis, KXRGG
Abstract:
目的：从细胞分子水平研究抗纤软肝冲剂对肝星状细胞(HSC)表达转化生长因子β1(TGF-β1)的影响。方法：抗纤软肝冲剂药物血清温育传一代HSC,以生理盐水和秋水仙碱为对照。免疫细胞化学分析TGF-β1的蛋白合成；半定量RT-PCR法检测TGF-β1的mRNA表达量。结果：抗纤软肝冲剂能明显抑制HSC的自分泌TGF-β1及其mRNA表达(P<0.01)。结论：抗纤软肝冲剂能抑制HSC的TGF-β1的基因和蛋白表达,阻断其自分泌放大活化效应,这可能是该方抗肝纤维化的细胞分子学机制之一
| [1] | bonaldomf,lennong,soaresmb.normalizationandsubtraction:twoapproachestofacilitategenediscovery.genomeres,1996,6:791
|
| [2] | 徐列明,刘成,刘平,等.一种稳定和高产的肝贮脂细胞分离法.细胞生物学杂志,1995,117(3)：14
|
| [3] | nakamurat,sakatar,uemot,etal.inhibitionoftransforminggrowthfactorbetapreventsprogressionofliverfibrosisandenhanceshepatocyteregenerationindimethylnitrosamine-treatedrats.hepatology,2000,32：247
|
| [4] | friedmansl.molecularmechanismsofhepaticfibrosisandprinciplesoftherapy.jgastroenterol,1997,32：4242flisiakr,pytel-krolczukb,prokopowiczd.circulatingtransforminggrowthfactorbeta(1)asanindicatorofhepaticfunctionimpairmentinlivercirrhosis.cytokine,2000,12：677
|
| [5] | iimuroy,galluccirm,lustermi,etal.antibodiestotumornecrosisfactoralfaattenuatehepaticnecrosisandinflammationcausedbychronicexposuretoethanolintherat.hepatology,1997,26：1530
|
| [6] | 王灵台,张斌.抗纤复方对大鼠肝贮脂细胞表达tgfβ1影响.中西医结合肝病志,1999,9：21
|
| [7] | gressneram.transdifferentiationofhepaticstellatecellstomyofibroblasts：akeyeventinhepaticfibrogenesis.kidneyintsuppl,1996,49：39
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
