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OALib Journal期刊
ISSN: 2333-9721
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eb病毒潜伏膜蛋白1基因多态性与nk/t细胞淋巴瘤的相关性
Keywords: eb病毒,淋巴瘤,结外nk/t细胞,潜伏膜蛋白1,基因多态性
Abstract:
？目的:探讨eb病毒(ebv)潜伏膜蛋白1(lmp1)基因变异与山东地区结外鼻型nk/t细胞淋巴瘤(enktl)的相关性。方法:采用巢式pcr和dna测序检测山东地区ebv阳性enktl组织(47例)和健康成年人咽漱液(70例)中lmp1全长序列,与ebv标准株及相关序列进行比对,绘制系统进化树,根据其特征性突变对基因变异进行分类,比较lmp1各变异类型在enktl和健康人群中分布的差异。结果:117例标本完成测序,共发现3种主要变异类型:china1、china2和med-,其在enktl中的检出率分别为85.1%、8.5%和4.3%,在健康人群中的检出率分别为65.7%、5.7%和21.4%,另有1例(2.1%)enktl和5例(7.2%)健康人群为不同亚型的重组病毒株,lmp1亚型在2种人群中的分布差异具有统计学意义(p=0.022)。lmp1n端xhoi位点缺失变异即xhoi(-)和c端30bp缺失即del-lmp1在enktl中的检出率(95.7%和85.1%)高于健康人群(74.3%和67.1%),差异均有统计学意义(p=0.003和p=0.029)。结论:山东地区enktl中lmp1以china1亚型、xhoi(-)和del-lmp1为主,带有3种变异型的ebv毒株可能与enktl的发生相关。
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