全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

甲醛对人ⅱ型肺泡上皮细胞dna氧化和甲基化水平的影响

Keywords: 甲醛,dna氧化,dna甲基化,8-羟基鸟嘌呤脱氧核苷,5-甲基胞嘧啶

Full-Text   Cite this paper   Add to My Lib

Abstract:

?目的:?分析甲醛染毒对体外培养的人ⅱ型肺泡上皮细胞(a549)内8-羟基鸟嘌呤脱氧核苷(8-oxo-dg)水平、dna?5-甲基胞嘧啶(5-mc)含量的影响及其时间-效应和剂量-效应关系,探讨甲醛所致的细胞dna氧化与dna甲基化的关联性。方法:用5~20μmol/l甲醛作用于a549细胞,用高压液相色谱串联电化学检测技术分析细胞dna?8-oxo-dg水平,用免疫荧光和高效毛细管电泳法检测细胞全基因组dna?5-mc含量改变。结果:甲醛染毒提高了细胞8-oxo-dg水平,但是降低了细胞的dna?5-mc含量。甲醛致细胞dna氧化与dna甲基化呈现不同特点,a549细胞暴露于20μmol/l甲醛后,dna氧化水平在1周甚至更早时间就已显著增高,而相同条件下dna甲基化水平的改变需要6周以上的时间,dna甲基化的改变滞后于dna氧化。a549细胞经5~20?μmol/l甲醛染毒8周后,dna氧化水平与甲醛剂量呈正相关关系,而同样条件下dna甲基化水平与甲醛剂量呈负相关关系。结论:甲醛染毒可提高dna氧化水平,降低细胞dna甲基化水平。在甲醛的毒作用效应中,细胞dna氧化损伤可能是早于其dna甲基化的一个重要分子事件。

References

[1]  owusu-ansahe,banerjeeu.reactiveoxygenspeciesprimedrosophilahaematopoieticprogenitorsfordifferentiation[j].nature,2009,461(7263):537-541.
[2]  kohkp,raoa.dnamethylationandmethylcytosineoxidationincellfatedecisions[j].curropincellbiol,2013,25(2):152-161.
[3]  武阳,常青,杨旭.不同浓度甲醛致大鼠肝细胞dna氧化损伤作用[j].环境科学学报,2009,29(11):2415-2419.
[4]  liuq,yangl,gongc,etal.effectsoflong-termlow-doseformaldehydeexposureonglobalgenomichypomethylationin16hbecells[j].toxicollett,2011,205(3):235-240.
[5]  netzern,goodenbourjm,davida,etal.innateimmuneandchemicallytriggeredoxidativestressmodifiestranslationalfidelity[j].nature,2009,462(7272):522-526.
[6]  hitchlermj,wikainapakulk,yul,etal.epigeneticregulationofmanganesesuperoxidedismutaseexpressioninhumanbreastcancercells[j].epigenetics,2006,1(4):163–171.
[7]  liul,liy,tollefsbolto.gene-environmentinteractionsandepigeneticbasisofhumandiseases[j].currissuesmolbiol,2008,10(1/2):25-36.
[8]  key,chengj,zhangz,etal.increasedlevelsofoxidativednadamageattributabletocookingoilfumesexposureamongcooks[j].inhaltoxicol,2009,21(8):682-687.
[9]  dengcx.brca1:cellcyclecheckpoint,geneticinstability,dnadamageresponseandcancerevolution[j].nucleicacidsres,2006,34(5):1416-1426.
[10]  nakaed,mizumotoy,kobayashie,etal.improvedgenomic/nucleardnaextractionfor8-hydroxyguanosineanalysisofsmellamountsofratlivertissue[j].cancerlett,1995,97(5):233-239.
[11]  张慧敏,陶功华,柯跃斌.毛细管胶束电动色谱测定基因组dna甲基化水平[j].中华预防医学杂志,2010,44(6):539-541.
[12]  klunglanda,bjellandbs.oxidativedamagetopurinesindna:roleofmammalianogg1[j].dnarepair,2007,6(4):481-488.
[13]  kovtuniv,liuy,bjorasm,etal.ogg1initiatesage-dependentcagtrinucleotideexpansioninsomaticcells[j].nature,2007,447(7143):447-452.
[14]  key,duanx,wenf,etal.associationofmelamineexposurewithurinarystoneandoxidativednadamageininfants[j].archtoxicol,2010,84(4):301-307.
[15]  lovellma,markesberywr.oxidativednadamageinmildcognitiveimpairmentandlate-stagealzheimer’sdisease[j].nucleicacidsres,2007,35(22):7497-7504.
[16]  bowermanb.oxidativestressandcancer[j].science,2005,308(20):1119-1121.
[17]  bernardd,gilj,dumontp,etal.themethyl-cpg-bindingproteinmecp2isrequiredforprostatecancercellgrowth[j].oncogene,2006,25(9):1358-1366.
[18]  tunco,tremellenk.oxidativednadamageimpairsglobalspermdnamethylationininfertilemen[j].jassistreprodgenet,2009,26(9/10):537–544.
[19]  柯跃斌,夏菠,梁明婵,等.dna去甲基化与基因激活过程[j].癌变·畸变·突变,2010,22(2):149-153.
[20]  gehringm,reikw,henikoffs.dnademethylationbydnarepair[j].trendsgenet,2009,25(2):82–90.
[21]  pastorwa,papeuj,huangy,etal.genome-widemappingof5-hydroxymethylcytosineinembryonicstemcells[j].nature,2011,473(7347):394-397.
[22]  wusc,zhangy.activednademethylation:manyroadsleadtorome[j].natrevmolcellbiol,2010,11(9):607-620.
[23]  yamaguchir,hiranot,asamis,etal.increased8-hydroxyguaninelevelsindnaanditsrepairactivityinratkidneyafteradministrationofarenalcarcinogen,ferricnitrilotriacetate[j].carcinogenesis,1996,17(11):2419-2422.
[24]  yamaguchir,hiranot,ootsuyamay,etal.increaseof8-hydroxyguanineindnaanditsrepairactivityinhamsterandratlungafterintratrachealinstillationofcrocidoliteasbestos[j].jpnjcancerres,1999,90(5):505-509.
[25]  ehrlichm,woodscb,yumc,etal.quantitativeanalysisofassociationsbetweendnahypermethylation,hypomethylation,anddnmtrnalevelsinovariantumors[j].oncogene,2006,25(18):2636-2645.
[26]  wangp,maiz,daiz,etal.investigationofdnamethylationbydirectelectrocatalyticoxidation[j].chemcommun,2010,46(41):7781–7783.
[27]  hutcgkermj,donmannf.anepigeneticperspectiveonthefreeradicaltheoryofdevelopment[j].freeradicbiolmed,2007,43(7):1023-1036.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133