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维吾尔族妇女宫颈病变与calca基因启动子甲基化的关系

Keywords: 降钙素相关肽&alpha,启动子,甲基化,维吾尔族妇女宫颈癌,cpg位点

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Abstract:

?目的:研究维吾尔族妇女宫颈病变与降钙素相关肽α(calcitonin-relatedpolypeptidealpha,calca)基因启动子区cpg位点甲基化的关系,进一步揭示宫颈癌组织中该基因表达调控的表观遗传学机制。方法:收集维吾尔族妇女宫颈病变患者手术切除或活检组织标本50例,其中宫颈鳞癌(cervicalsquamouscellcarcinoma,cscc)25例、宫颈内上皮内瘤样变(cervicalintraepithelialneoplasia,cin)9例及慢性宫颈炎16例,应用sequenommassarray甲基化dna定量分析平台,对组织dna的calca基因启动子区cpg位点进行甲基化水平的定量分析。结果:宫颈炎、cin和cscc组织中calca基因启动子区cpg片段甲基化率分别为0.22、0.26和0.33,经统计学分析,cscc与cin以及cscc与宫颈炎之间均有显著差异(p<0.05)。从单一cpg位点角度分析,cscc与宫颈炎组织cpg-2、cpg-3、cpg-9.10.11和cpg-12等6个cpg位点甲基化率均有显著差异(p<0.05)。结论:calca基因启动子甲基化与维吾尔族妇女宫颈癌发生密切相关,其中特定cpg位点甲基化水平变化可能成为宫颈癌早期诊断的分子标志物。

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