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2,4-d丁酯对金鱼血清过氧化物酶和酯酶同工酶的影响

Keywords: 2,4-d丁酯,金鱼,血清,过氧化物酶,酯酶

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Abstract:

?目的:探讨除草剂2,4-d丁酯对金鱼血清过氧化物酶(pod)和酯酶(est)同工酶的影响。方法:根据半数致死浓度,设置0.5、1.0、1.5、2.0ml/l?4个浓度组及0.9%生理盐水对照组,每个浓度组按0.1ml/g腹腔注射金鱼,染毒24h,断尾取血,离心,取上清液,聚丙烯酰胺方法检测金鱼血清pod和est同工酶活性,实验数据采用anova分析。结果:与对照组相比,2,4-d丁酯在0.5、1.5和2.0ml/l浓度时可抑制金鱼血清pod、est同工酶活性(p<0.05),在1.0ml/l浓度时可使金鱼血清est同工酶活性增强(p<0.05)。2,4-d丁酯对est同工酶迁移率的影响大于pod。结论:2,4-d丁酯对金鱼血清pod和est同工酶活性均有影响,2,4-d丁酯可改变est同工酶的迁移率。

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