|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
柔细束丝藻土臭素合成酶基因的克隆及表达分析
DOI: 10.3724/SP.J.1145.2015.04011, PP. 824-829
Keywords: 高效热不对称交错pcr,柔细束丝藻,土臭素合成酶,异味物质
Abstract:
土臭素（geosmin）是一种具有土霉异味的次级代谢产物，对水体具有严重的影响.为探讨柔细束丝藻（aphanizomenongracile）土臭素的合成机制以及环境因子对其土臭素产量的影响，利用高效热不对称交错pcr（high-efficiencythermalasymmetricinterlacedpcr，hitail-pcr）技术对柔细束丝藻藻株wh-1的土臭素合成酶基因进行克隆，并对该基因在不同温度和光强下的表达情况进行实时荧光定量pcr（rt-pcr）分析.结果显示本研究成功克隆柔细束丝藻藻株wh-1的土臭素合成酶基因（geo），geo全长2262bp，编码753个氨基酸.低温（15℃）、强光（35μmolm-2s-1）以及不间断光照处理均有利于geo的表达.这表明hitail-pcr技术作为一种高效、低廉的染色体步移技术具有较好的应用价值，同时为异味物质的监测及防治提供部分理论基础.
| [1] | 1petera,kostero,schildknechta,vonguntenu.occurrenceofdissolvedandparticle-boundtasteandodorcompoundsinswisslakewaters[j].waterres,2009,43(8):2191-2200
|
| [2] | 2durrerm,zimmermannu,juttnerf.dissolvedandparticle-boundgeosmininamesotrophiclake(lakezurich):spatialandseasonaldistributionandtheeffectofgrazers[j].waterres,1999,33(17):3628-3636
|
| [3] | 3zhangt,lil,zuoy,zhouq,songl.biologicaloriginsandannualvariationsofearthy-mustyoff-flavoursinthexionghereservoirinchina[j].jwatersupply:restechnol,2010,59(4):243-254
|
| [4] | 4gerbern,lechevalierh.geosmin,anearthy-smellingsubstanceisolatedfromactinomycetes[j].applmicrobiol,1965,13(6):935-938
|
| [5] | 5canede,wattrm.expressionandmechanisticanalysisofagermacradienolsynthasefromstreptomycescoelicolorimplicatedingeosminbiosynthesis[j].pnatlacadsciusa,2003,100(4):1547-1551
|
| [6] | 6jiangj,hex,canede.geosminbiosynthesis.streptomycescoelicolorgermacradienol/germacrenedsynthaseconvertsfarnesyldiphosphatetogeosmin[j].jamchemsoc,2006,128(25):8128-8129
|
| [7] | 14ochmanh,gerberas,hartldl.geneticapplicationsofaninversepolymerasechainreaction[j].genetics,1988,120(3):621-623
|
| [8] | 15trouttab,mcheyzer-williamsmg,pulendranb,nossalg.ligation-anchoredpcr:asimpleamplificationtechniquewithsingle-sidedspecificity[j].pnatlacadsciusa,1992,89(20):9823-9825
|
| [9] | 16王闵霞,马欣荣,王天山,谭红.染色体步行pcr技术[j].应用与环境生物学报,2006,12(3):427-430[wangmx,maxr,wangts,tanh.pcrtechniquesforchromosomewalking[j].chinjapplenvironbiol,2006,12(3):427-
|
| [10] | 17liuy-g,cheny.high-efficiencythermalasymmetricinterlacedpcrforamplificationofunknownflankingsequences[j].biotechniques,2007,43(5):649-656
|
| [11] | 18lil,pangd,wangt,liz,chenl,zhangm,songn,nied,chenz,lail.productionofareportertransgenicpigformonitoringcrerecombinaseactivity[j].biochembiophresco,2009,382(2):232-235
|
| [12] | 20yangj,lij,maiz,tianx,zhangs.purification,characterization,andgenecloningofacold-adaptedthermolysin-likeproteasefromhalobacillussp.scsio20089[j].jbioscibioen,2013,115(6):628-632
|
| [13] | 23porebskis,baileylg,baumbr.modificationofactabdnaextractionprotocolforplantscontaininghighpolysaccharideandpolyphenolcomponents[j].plantmolbiolrep,1997,15(1):8-15
|
| [14] | 24daumm,herrmanns,wilkinsonb,bechtholda.genesandenzymesinvolvedinbacterialisoprenoidbiosynthesis[j].curropinchembiol,2009,13(2):180-188
|
| [15] | 25zhangt,lil,songl,chenw.effectsoftemperatureandlightonthegrowthandgeosminproductionoflyngbyakuetzingii(cyanophyta)[j].japplphycol,2009,21(3):279-285
|
| [16] | 26saadounimk,schraderkk,blevinswt.environmentalandnutritionalfactorsaffectinggeosminsynthesisbyanabaenasp.[j].waterres,2001,35(5):1209-1218
|
| [17] | 27naesh,aarnesh,utkilenh,nilsens,skulbergo.effectofphotonfluencerateandspecificgrowthrateongeosminproductionofthecyanobacteriumoscillatoriabrevis(kütz.)gom[j].applenvironmicrob,1985,49(6):1538
|
| [18] | 28bowmerk,padovana,oliverr,korthw,ganfg.physiologyofgeosminproductionbyanabaenacircinalisisolatedfromthemurrumbidgeeriver,australia[j].waterscitechnol,1992,25(2):259-267
|
| [19] | 7jiangj,hex,canede.biosynthesisoftheearthyodorantgeosminbyabifunctionalstreptomycescoelicolorenzyme[j].natchembiol,2007,3(11):711-715
|
| [20] | 8ludwigf,medgera,boernickh,opitzm,langk,goettfertm,roeskeli.identificationandexpressionanalysesofputativesesquiterpenesynthasegenesinphormidiumsp.andprevalenceofgeoa-likegenesinadrinkingwaterreservoir[j].applenvironmicrob,2007,73(21):6988-6993
|
| [21] | 9giglios,jiangj,saintcp,canede,monispt.isolationandcharacterizationofthegeneassociatedwithgeosminproductionincyanobacteria[j].environscitechnol,2008,42(21):8027-8032
|
| [22] | 10aggersa,lopez-gallegof,hoyetr,schmidt-dannertc.identificationofsesquiterpenesynthasesfromnostocpunctiformepcc73102andnostocsp.strainpcc7120[j].jbacteriol,2008,190(18):6084-6096
|
| [23] | 11giglios,saintcp,monispt.expressionofthegeosminsynthasegeneinthecyanobacteriumanabaenacircinalisawqc318[j].jphycol,2011,47(6):1338-1343
|
| [24] | 12王中杰.蓝藻土霉异味物质合成基因的研究及其生态应用[d].武汉:中国科学院水生生物研究所,2012[wangzj,thestudiesongenesresponsibleforsynthesisofearthy-mustyodorantsincyanobacteriaandtheirecologicalapplication[d].wuhan:instituteofhydrobiology,chineseacademyofsciences,
|
| [25] | 13zhangt,lid,wangg,songl,lil.identificationandexpressionanalysisofthegeneassociatedwithgeosminproductioninlyngbyakuetzingiiutex1547(cyanobacteria)[j].harmfulalgae,2014,39:127-133
|
| [26] | 19ruiz-rojasj,sargentd,shulaevv,dickermana,pattisonj,holts,ciordiaa,veilleuxre.snpdiscoveryandgeneticmappingoft-dnainsertionalmutantsinfragariavescal.[j].theorapplgenet,2010,121(3):449-463
|
| [27] | 21wangg-h,xiaoj-h,xiongt-l,liz,murphyrw,huangd-w.determinationofahighlydegeneratedprophagewogenomeinthewolbachiastrainofafigwaspspeciesbyhitail-pcr[j].applenvironmicrob,201381(19):2261-2213
|
| [28] | 22周莉莉,陈瑞琴,陈秀兰,曾胤新,陈波.应用hitail-pcr技术克隆marinomonassp.bsi20584菌株β-半乳糖苷酶基因[j].极地研究,2013,12(3):249-256[zhoull,chenrq,chenxl,zengyx,chenb.cloningofβ-galactosidasegenesfrommarinomonassp.bsi20584byhitail-pcr[j].chinjpolarres,2013,12(3):249-
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
