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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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细菌dietziasp.dq12-45-1b中两种长链烷烃羟化酶基因的鉴定
DOI: 10.3724/SP.J.1145.2015.04039, PP. 813-818
Keywords: dietziasp.,长链烷烃,alma,lada,生物降解
Abstract:
细菌dietziasp.dq12-45-1b是一株高效石油降解菌，从中已经鉴定了编码膜整合烷烃羟化酶（alkb）和细胞色素p450类cyp153烷烃羟化酶的基因alkw1和cyp153，该菌能产生不同的表面活性剂以降解不同碳链长度的烷烃，但在不同时期对不同碳源进行降解的机制仍不清楚.通过以二十八烷（c28）为单一碳源培养alkw1缺陷的突变株m5-5，发现alkw1缺陷降低了菌株对c28的降解能力，但仍具有降解c28的能力.通过对dq12-45-1b菌株全基因组信息分析，并运用实时荧光定量pcr（real-timepcr）技术，鉴定了两种长链烷烃羟化酶基因，其中g1的氨基酸序列与acinetobactersp.dsm17874alma的相似性是50%，g2的氨基酸序列与geobacillusthermodenitrificansng80-2lada的相似性是48%.同时，发现dietziasp.e1存在基因g1的同源基因（g1α）和g2的同源基因（g2α和g2β），其中基因g1α与g1的相似性是71%，基因g2α、g2β与g2的相似性分别是34%、87%.real-timepcr分析表明，在dietziasp.dq12-45-1b及突变株m5-5中，基因g1和g2的转录水平均因c28的诱导而显著上调.上述对alma和lada同源基因的鉴定表明，dietziasp.dq12-45-1b具有独特的烷烃降解多酶体系，是目前报道的烷烃羟化酶最多的细菌之一.
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