|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
基于叶绿体psba-trnh和核糖体5srrna基因间隔区序列的石斛种间和种内鉴别
DOI: 10.3724/SP.J.1145.2015.03026, PP. 887-896
Keywords: 石斛,psba-trnh基因间隔区序列,5srrna基因间隔区序列,多序列组合,分子鉴别,单核苷酸多态性位点
Abstract:
采用直接测序法对12种石斛叶绿体psba-trnh基因间隔区序列和核糖体5srrna基因间隔区序列进行测序，用克隆测序法对叠鞘石斛dq-2品种和dq-5品系（各20份样品）psba-trnh基因间隔区序列进行测序，所得序列采用squencher4.14拼接后，采用bioedit7.0软件对获得的psba-trnh基因间隔区序列和5srrna基因间隔区序列进行序列比对和排序，用dnasp5.0软件分析序列核苷酸多态性，用mega5.2计算种内和种间kimura2-parameter（k2p）遗传距离，并构建系统发育树（nj树）.结果显示，石斛叶绿体psba-trnh基因间隔区序列平均长度为742.3bp，有72个变异位点，其中信息位点33个；核糖体5srrna基因间隔区序列平均长度为336.4bp，存在213个变异位点，其中信息位点139个；以上两个基因片段的组合序列，能对叠鞘石斛、铁皮石斛、球花石斛、细叶石斛、细茎石斛、齿瓣石斛、鼓槌石斛、尖刀唇石斛和金钗石斛等9种石斛进行有效鉴别，可揭示铁皮石斛不同居群间的差异，而且可根据该组合序列中存在的一个单核苷酸多态性位点（singlenucleotidepolymorphism，snp）对叠鞘石斛dq-2品种和dq-5品系进行区分.本研究表明，psba-trnh基因间隔区序列和5srrna基因间隔区序列组合后显示出多序列组合策略在石斛种间和种内的分子鉴别中的优势，可为石斛属植物及药材鉴别提供分子依据.
| [1] | 1何涛,淳泽,罗傲雪,范益军,刘静,胡明珠.四川石斛野生资源及其保护研究[j].应用与环境生物学报,2008,14(5):710-715[het,chunz,luoax,fanyj,liuj,humz.wildresourcesandconservationofdendrobiuminsichuanchina[j].chinjapplenvironbiol,2008,14(5):710-
|
| [2] | 2刘静,何涛,淳泽.分子标记技术在石斛属植物中的应用研究进展[j].应用与环境生物学报,2008,14(6):855-862[liuj,het,chunz.advanceinapplicationofdnamolecularmarkersondendrobium[j].chinjapplenvironbiol,2008,14(6):855-
|
| [3] | 3suchernj,carlesmc.genome-basedapproachestotheauthenticationofmedicinalplants[j].plantamed,2008,74:603-623
|
| [4] | 8曾丽萍,张宁,马红.被子植物系统发育深层关系研究:进展与挑战[j].生物多样性,2014,22(1):21-39[zenglp,zhangn,mah.advancesandchallengesinresolvingtheangiospermphylogeny[j].biodivsci,2014,22(1):21-
|
| [5] | 9dongwp,liuj,yuj,wangl,zhousl.highlyvariablechloroplastmarkersforevaluatingplantphylogenyatlowtaxonomiclevelsandfordnabarcoding[j].plosone,2012,7(4):e35071
|
| [6] | 24berteacm,azzolincmm,bossis,dogliag,maffeim.identificationofanecorirestrictionsiteforarapidandprecisedeterminationofbasarone-freeacoruscalamuscytotypes[j].phytochemistry,2005,66:507-514
|
| [7] | 25?torchováh,olsonms.thearchitectureofthechloroplastpsba-trnhnon-codingregioninangiosperms[j].plantsystevol,2007,268(1-4):235-256
|
| [8] | 26钟珍萍,吴乃虎.应用体外系统分析水稻高粱psba3′utr调控效应[j].中国科学(c辑),1997,27(3):247
|
| [9] | 27wunh,c?téjc,wur.structureofthechloroplastpsbageneencodingtheqbproteinfromoryzasatival.[j].devgenet,1987,8:339-350
|
| [10] | 29fazekasaj,burgessks,kesanakurtipr,grahamsw,newmastersg,husbandbc,percydm,hajibabaeim,barrettsch.multiplemultilocusdnabarcodesfromtheplastidgenomediscriminateplantspeciesequallywell[j].plosone,2008,3(7):e2802
|
| [11] | 30栗丹,李振坚,毛萍,严雪锋,淳泽,马欣荣.基于its序列石斛材料的鉴定及系统进化分析[j].园艺学报2012,39(8):1539-1550[lid,lizj,maop,yanxf,chunz,maxr.phylogeneticanalysisandidentificationofdendrobiumspeciesbasedonribosomaldnainternaltranscribedspacer(its)sequence[j].actahortsin,2012,39(8):1539-
|
| [12] | 31钟珍萍,吴乃虎.叶绿体基因mrna3’utr功能[j].生物工程进展,1995,15(2):18-22
|
| [13] | 32钟珍萍,吴乃虎.真核生物mrna稳定性的机制[j].生物工程进展,1997,17(3):34-38[zhongzp,wunh.stabilityofmrnaineukaryote[j].progbiotechnol,1997,17(3):34-
|
| [14] | 4刘静,何涛,淳泽.药用石斛的叶绿体matk基因序列分析及鉴别[j].药学学报,2009,44(9):1051-105[liuj,het,chunz.analysisandauthenticationofchloroplastmatkgenesequencesofherbadendrobii[j].actapharmsin,2009,44(9):1051-
|
| [15] | 5刘静,何涛,淳泽.基于its序列的中国药用石斛及其混伪品的分子鉴定[j].中国中药杂志,2009,34(22):2853-2856[liuj,het,chunz.dnamolecularidentificationofherbadendrobiianditsadulterantspeciesbasedonitssequenceanalysis[j].chinjchinmatermed,2009,34(22):2853-
|
| [16] | 6singhhk,parveeni,raghuvanshis,babbarsb.thelocirecommendedasuniversalbarcodesforplantsonthebasisoffloristicstudiesmaynotworkwithcongenericspeciesasexemplifiedbydnabarcodingofdendrobiumspecies[j].bmcresnot,2012,5:42
|
| [17] | 7宁淑萍,颜海飞,郝刚,葛学军.植物dna条形码研究进展[j].生物多样性,2008,16(5):417-425[ningsp,yanhf,haog,gexj.currentadvancesofdnabarcodingstudyinplants[j].biodivsci,2008,16(5):417-
|
| [18] | 10zhangn,zenglp,shanhy,mah.highlyconservedlow-copynucleargenesaseffectivemarkersforphylogeneticanalysesinangiosperms[j].nphytol,2012,195:923-937
|
| [19] | 11lixw,yangy,henryrj,rossettom,wangyt,chensl.plantdnabarcoding:fromgenetogenome[j].biolrevcambphilossoc,2014(1):157-166
|
| [20] | 12?lvarezi,wendeljf.ribosomalitssequencesandplantphylogeneticinference[j].molphylogenetevol,2003,29:417-434
|
| [21] | 13newmastersg,fazekasaj,steevesrad,janovecj.testingcandidateplantbarcoderegionsinthemyristicaceae[j].molecolres,2008,8:480-490
|
| [22] | 14yaoh,songjy,maxy,liuc,liy,xuhx,hanjp,duanls,chensl.identificationofdendrobiumspeciesbyacandidatednabarcodesequence:thechloroplastpsba-trnhintergenicregion[j].plantamed,2009,75:667-669
|
| [23] | 15horih,osawats.originandevolutionoforganismsasdeducedfrom5sribosomalrnasequences[j].molbiolevol,1987,4(5):445-472
|
| [24] | 16chenf,chanhye,wongkl,wangj,yumt,butpph,shawpc.authenticationofsausssrealappa,anendangeredmedicinalmaterial,byitsdnaand5s-rrna[j].plantamed,2008,74:889-892
|
| [25] | 17dongtt,maxq,clarkec,songzh,jizn,lock.phylogenyofastragalusinchina:molecularevidencefromthednasequencesof5srrnaspacer,its,and18srrna[j].agricfoodchem,2003,51:6709-6714
|
| [26] | 18caizh,lip,dongttx,tsimkwk.moleculardiversityof5s-rrnaspacerdomaininfririllariaspeciesrevealedbypcranalysis[j].plantamed,1999,65:360-364
|
| [27] | 19ganalmw,lapitannlv,tanksleysd.amolecularandcytogeneticsurveyofmajorrepeateddnasequencesintomato(lycopersiconesculentum)[j].molgengenet,1988,213:262-268
|
| [28] | 20negims,rajagopalj,chauhann,cronnr,lakshmikumaranm.lengthandsequenceheterogeneityin5srdnaofpopulousdeltoids[j].genome,2002,45:1181-1188
|
| [29] | 21pinhald,yoshimuraas,arakics,martinsc.the5srdnafamilyevolvesthroughconcertedandbirth-and-deathevolutioninfishgenomes:anexamplefromfreshwaterstingrays[j].bmcevolbiol,2011,11(2):131-146
|
| [30] | 22doylejj,doylejl.arapidisolationprocedureforsmallquantitiesoffreshleaftissue[j].phytochembull,1987,19:11-15
|
| [31] | 23kresswj,ericksondl.atwo-locusglobaldnabarcodeforlandplants:thecodingrbclgenecomplementsthenon-codingtrnh-psbaspacerregion[j].plosone,2007,6:e508
|
| [32] | 28吴乃虎,方晓华,施晓梅,张晓武,周立,黄美娟,孙凯鸣.高粱叶绿体psba基因的结构特征及5′utr调控效应[j].中国科学(c辑),1999,29(4):397-406
|
| [33] | 33adamscc,sterndb.controlofmrnastabilityinchloroplastsby3’invertedrepeats:effectsofstemandloopmutationsondegradationofpsbamrnainvitro[j].nucleicacidsres,1990,18(20):6003-6010
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
