|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
油松林地点柄乳牛肝菌菌塘细菌群落动态
DOI: 10.3724/SP.J.1145.2015.03001, PP. 974-980
Keywords: 点柄乳牛肝菌,16srdna,pcr-dgge,细菌群落,出菇期
Abstract:
为了解北京山区油松（pinustabuliformis）林地点柄乳牛肝菌（suillusgranulatus）出菇期间菌塘内的细菌群落变化规律，收集出菇期前后（5-11月）的菌塘土样，通过聚合酶链式反应-变性梯度凝胶电泳（pcr-dgge）方法进行评估与分析.利用土壤总dna提取试剂盒提取不同时期总dna，以细菌通用引物338f和518r进行pcr扩增和dgge，进一步对细菌群落的dgge指纹图谱分析后进行切胶回收和测序.结果显示在点柄乳牛肝菌出菇期间，菌塘中细菌群落较为丰富，群落多样性指数维持在较高水准，并且菌塘中的细菌群落呈现出季节性变化.菌塘细菌分属于5个门（酸杆菌门、放线菌门、拟杆菌门、厚壁菌门和变形菌门）22个属（酸杆菌属、氨基酸杆菌属等）.菌塘中变形菌门和酸杆菌门菌群的相对丰度较高，为优势菌群，其中α-变形菌纲为菌塘中最丰富的菌群.γ-变形菌纲菌群的相对丰度变化与点柄乳牛肝菌子实体发生周期一致.酸杆菌门菌群的相对丰度在点柄乳牛肝菌出菇前期（7月）明显增大.这些发现为点柄乳牛肝菌野生资源保育与人工驯化等后续研究提供了理论和实验依据.
| [1] | 1kivlinsn,hawkescv,tresederkk.globaldiversityanddistributionofarbuscularmycorrhizalfungi[j].soilbiolbiochem,2011,43(11):2294-2303
|
| [2] | 2brundrettm.diversityandclassificationofmycorrhizalassociations[j].biolrevcambphilossoc,2004,79(3):473-495
|
| [3] | 3tojuh,satoh,tanabeas.diversityandspatialstructureofbelowgroundplant-fungalsymbiosisinamixedsubtropicalforestofectomycorrhizalandarbuscularmycorrhizalplants[j].plosone,2014,9(1):e86566
|
| [4] | 4mongodinef,emersonjb,nelsonke.microbialmetagenomics[j].genomebiol,2005,6(10):347
|
| [5] | 5lechateliere,nielsent,qinj,priftie,hildebrandf,falonyg,almeidam,arumugamm,battojm,kennedys,leonardp,lij,burgdorfk,grarupn,jorgensent,brandslundi,nielsenhb,junckeras,bertalanm,levenezf,ponsn,rasmussens,sunagawas,tapj,timss,zoetendaleg,brunaks,clementk,dorej,kleerebezemm,kristiansenk,renaultp,sicheritz-pontent,devoswm,zuckerjd,raesj,hansent,metahitc,borkp,wangj,ehrlichsd,pederseno.richnessofhumangutmicrobiomecorrelateswithmetabolicmarkers[j].nature,2013,500(7464):541-546
|
| [6] | 6delongef.microbialcommunitygenomicsintheocean[j].natrevmicrobiol,2005,3(6):459-469
|
| [7] | 7viku,logaresr,blaalidr,halvorsenr,carlsent,bakkei,kolstoab,okstadoa,kauserudh.differentbacterialcommunitiesinectomycorrhizaeandsurroundingsoil[j].scirep-uk,2013,3:3471
|
| [8] | 8yangq,wangj,wangh,chenx,rens,lix,xuy,zhangh,lix.evolutionofthemicrobialcommunityinafull-scaleprintinganddyeingwastewatertreatmentsystem[j].bioresourtechnol,2012,117:155-163
|
| [9] | 9wernerjj,knightsd,garciaml,scalfonenb,smiths,yarasheskik,cummingsta,beersar,knightr,angenentlt.bacterialcommunitystructuresareuniqueandresilientinfull-scalebioenergysystems[j].procnatlacadsciusa,2011,108(10):4158-4163
|
| [10] | 10muyzerg,dewaalec,uitterlindenag.profilingofcomplexmicrobialpopulationsbydenaturinggradientgelelectrophoresisanalysisofpolymerasechainreaction-amplifiedgenescodingfor16srrna[j].applenvironmicrobiol,1993,59(3):695-700
|
| [11] | 11andersonic,cairneyjw.diversityandecologyofsoilfungalcommunities:increasedunderstandingthroughtheapplicationofmoleculartechniques[j].environmicrobiol,2004,6(8):769-779
|
| [12] | 16narak.ectomycorrhizalnetworksandseedlingestablishmentduringearlyprimarysuccession[j].newphytol,2006,169(1):169-178
|
| [13] | 17rincona,ruiz-diezb,garcia-frailes,garciaja,fernandez-pascualm,pueyojj,defelipemr.colonisationofpinushalepensisrootsbypseudomonasfluorescensandinteractionwiththeectomycorrhizalfungussuillusgranulatus[j].femsmicrobiolecol,2005,51(3):303-311
|
| [14] | 20chenqj,niuyr,haoc,zhanggq,qinl,lijh,lius,weihl.occurrenceofediblemycorrhizalmushroominthepineforestsofbeijingmountainousarea[j].sciagricsin,2011,44(16):3377-3385
|
| [15] | 21zengg,yuz,cheny,zhangj,lih,yum,zhaom.responseofcompostmaturityandmicrobialcommunitycompositiontopentachlorophenol(pcp)-contaminatedsoilduringcomposting[j].bioresourtechnol,2011,102(10):5905-5911
|
| [16] | 36rappems,giovannonisj.theunculturedmicrobialmajority[j].annurevmicrobiol,2003,57:369-394
|
| [17] | 12carvalhaislc,dennispg,tysongw,schenkpm.applicationofmetatranscriptomicstosoilenvironments[j].jmicrobiolmeth,2012,91(2):246-251
|
| [18] | 13dickgj,anderssonaf,bakerbj,simmonssl,thomasbc,yeltonap,banfieldjf.community-wideanalysisofmicrobialgenomesequencesignatures[j].genomebiol,2009,10(8):r85
|
| [19] | 14ercolinid.pcr-dggefingerprinting:novelstrategiesfordetectionofmicrobesinfood[j].jmicrobiolmeth,2004,56(3):297-314
|
| [20] | 15diases,araujoef,guimaraeswv,muchovejrm.productionandregenerationofprotoplastsfromthemycorrhizalfungussuillusgranulatus[j].worldjmicrobbiot,1996,12(6):625-628
|
| [21] | 18lix,jinx,liqiang,xiongc,zhengl.effectsofecologicalenvironmentfactorsonsoilmicroorganismsoftricholomamatsutakeshiroinsichuanprovince[j].chinjapplenvironbiol,2015,21(1):164-169
|
| [22] | 19ogawam.microbialecologyof“shiro”intricholomamatsutake(ito.etimai)sing.anditsalliedspecies.vtricholomamatsutakeintsugasieboldiiforests[j].transmycolsocjpn,1977,18,:34-36
|
| [23] | 22schabereiter-gurtnerc,macas,kaminskys,rollekes,lubitzw,barisani-asenbauert.investigationofananaerobicmicrobialcommunityassociatedwithacornealulcerbydenaturinggradientgelelectrophoresisand16srdnasequenceanalysis[j].diagnmicrobinfecdis,2002,43(3):193-199
|
| [24] | 23garbevap,vanveenja,vanelsasjd.microbialdiversityinsoil:selectionmicrobialpopulationsbyplantandsoiltypeandimplicationsfordiseasesuppressiveness[j].annurevphytopathol,2004,42:243-270
|
| [25] | 24kirkjl,beaudettela,hartm,moutoglisp,klironomosjn,leeh,trevorsjt.methodsofstudyingsoilmicrobialdiversity[j].jmicrobiolmeth,2004,58(2):169-188
|
| [26] | 25zhaof,xuk.microbialgeneticdiversityandciliatecommunitystructurealonganenvironmentalgradientincoastalsoil[j].eurjprotistol,2013,49(4):516-525
|
| [27] | 26wangp,liuy,yiny,jinh,wangs,xuf,zhaos,gengx.diversityofmicroorganismsisolatedfromthesoilsamplesurroundchroogomphusrutilusinthebeijingregion[j].intjbiolsci,2011,7(2):209-220
|
| [28] | 27leekb,liuct,anzaiy,kimh,aonot,oyaizuh.thehierarchicalsystemofthe‘alphaproteobacteria’:descriptionofhyphomonadaceaefam.nov.,xanthobacteraceaefam.nov.anderythrobacteraceaefam.nov[j].intjsystevolmicrob,2005,55(pt5):1907-1919
|
| [29] | 28asakawas,kimuram.comparisonofbacterialcommunitystructuresatmainhabitatsinpaddyfieldecosystembasedondggeanalysis[j].soilbiolbiochem,2008,40:1322-1329
|
| [30] | 29gelsominoa,caccog.compositionalshiftsofbacterialgroupsinasolarizedandamendedsoilasdeterminedbydenaturinggradientgelelectrophoresis[j].soilbiolbiochem,2006,38:91-102
|
| [31] | 30xuy,wangg,jinj,liuj,zhangq,liux.bacterialcommunitiesinsoybeanrhizosphereinresponsetosoiltype,soybeangenotype,andtheirgrowthstage[j].soilbiolbiochem,2009,41:919-925
|
| [32] | 31niuy,wangm,zhangg,chenq,lius,jiangw.itssequenceanalysisofstrainobtainedfromwildsuillusgranulatusfruitbodiesandoptimizationofselectedparameters[j].jediblefungi,2009,20(4):6-10
|
| [33] | 32barnssm,cainec,sommervillel,kuskecr.acidobacteriaphylumsequencesinuranium-contaminatedsubsurfacesedimentsgreatlyexpandtheknowndiversitywithinthephylum[j].applenvironmicrobiol,2007,73(9):3113-3116
|
| [34] | 33quaisera,ochsenreitert,lanzc,schustersc,treuschah,eckj,schleperc.acidobacteriaformacoherentbuthighlydiversegroupwithinthebacterialdomain:evidencefromenvironmentalgenomics[j].molmicrobiol,2003,50(2):563-575
|
| [35] | 34eichorstsa,breznakja,schmidttm.isolationandcharacterizationofsoilbacteriathatdefineterriglobusgen.nov.,inthephylumacidobacteria[j].applenvironmicrobiol,2007,73(8):2708-2717
|
| [36] | 35kuskecr,barnssm,buschjd.diverseuncultivatedbacterialgroupsfromsoilsofthearidsouthwesternunitedstatesthatarepresentinmanygeographicregions[j].applenvironmicrobiol,1997,63(9):3614-3621
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
