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OALib Journal期刊
ISSN: 2333-9721
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基于高通量测序的杨树人工林根际和非根际细菌群落结构比较
DOI: 10.3724/SP.J.1145.2015.03018, PP. 967-973
Keywords: 杨树人工林,根际效应,细菌群落多样性,454高通量测序
Abstract:
为准确分析定殖在根际和非根际土壤的细菌类群组成，采用454高通量测序技术，对杨树人工林根际和非根际细菌群落进行对比分析.本次测序共获得原始序列70081条，过滤后序列总数为44009条.物种注释结果显示，根际土壤包含145个细菌属，非根际土壤包含141个细菌属.根际和非根际相对丰度＞4%的属有8个，它们是acidobacteriumgp1、acidobacteriumgp3、acidobacteriumgp6、gemmatimonas、bradyrhizobium、burkholderia、streptomyces和acidobacteriumgp4，同一属的细菌类群在根际和非根际土壤中相对丰度存在显著差异（p<0.05).α多样性分析表明，根际土壤细菌群落多样性高于非根际土壤，但差异未达到显著水平.细菌群落排序结果较好地反映了土壤细菌群落从根际到非根际的变化和不同取样点的空间差异，根际效应对细菌群落差异的贡献率占21.2%.β多样性分析表明，杨树人工林根际和非根际土壤样品间细菌群落组成具有较大差异，根际和非根际土壤中各含有一些特有属（根际15个，非根际11个），此外还发现丰度发生显著变化的23个属，主要以纤维素降解菌和固氮菌属为主.本研究表明，根系对根际微生物的选择是导致根际微生物群落组成和结构发生显著差异的重要机制，这将对根土界面碳氮循环产生显著影响.
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