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OALib Journal期刊
ISSN: 2333-9721
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真养产碱杆菌atcc17699生产聚β-羟基丁酸摇瓶发酵条件的研究
, PP. 308-314
Keywords: 真养产碱杆菌,聚β-羟基丁酸,发酵
Abstract:
在摇瓶条件下,首先对a.eutrophus生产phb的一步法和二步法进行了比较研究,然后探讨了a.eutrophus对不同碳源的利用情况,提出了较佳的phb发酵条件,并分析了phb的发酵过程曲线。研究结果表明,本研究采用的a.eutrophus以一步法生产phb较为适宜,其对3种不同碳源的利用能力为:果糖>丁酸>葡萄糖;培养基中的丁酸浓度和ph值对phb发酵的生产指标和动力学参数有较大影响,其最佳值为1.8%φ和ph为0.8;最终发酵过程达到细胞于重ρ=7.2g？l-1,胞内phb含量ρ=5.0g？l-1,ω(phb)占细胞干重72.2%.
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