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OALib Journal期刊
ISSN: 2333-9721
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一株中度嗜盐菌的分离及生物学特性
DOI: 10.3724/SP.J.1145.2012.00477, PP. 477-481
Keywords: 中度嗜盐菌,海杆菌属,脂肪酸成分,16srrna基因,生物学特性
Abstract:
从我国内蒙古吉兰泰盐湖的样品中分离得到一株中度嗜盐细菌菌株jlt-01，研究了其形态和特性.结果显示：该菌株为杆状，革兰氏阴性，无芽孢，无荚膜，无鞭毛；最适培养温度为30℃，最适生长ph值7.5，最适生长盐度10%~12%（m/v），最适生长mg2+浓度3.0%（m/v）；能利用葡萄糖、蔗糖、半乳糖作为唯一碳源生长.脂肪酸成分分析表明在jlt-01中主要脂肪酸为c16:0与c18:1ω9c，含量分别为26.39%和20.30%.tm法测定该菌的（g+c）含量（摩尔分数）为52.3%.以该菌的16srrna基因序列为基础构建了系统发育树；16srrna基因序列的序列同源性比对表明该菌与海杆菌属marinobacterlipolyticussm19t的同源性为98.0%，与marinobacter属内其它菌株的同源性在96.0%~99.0%之间.dna-dna杂交实验分析显示：jlt-01与m.lipolyticusdsm15157的杂交率小于70%.结果表明，菌株jlt-01是海杆菌属的一个新菌株.
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