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OALib Journal期刊
ISSN: 2333-9721
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不同生境产脂肪酶菌株的筛选及多样性
DOI: 10.3724/SP.J.1145.2012.00455, PP. 455-459
Keywords: 产脂肪酶菌株,碱性脂肪酶,eric-pcr,青海高寒草地
Abstract:
为发掘不同特性脂肪酶微生物资源，采用纯培养方法从青海高寒草地、雅安山区、成都平原分离得到产脂肪酶菌株54株.通过对菌株的dna进行eric-pcr指纹图谱分析，按聚类树划分为5个操作分类单元.16srdna序列测定和聚类分析显示，分离菌株分布于芽孢杆菌属（bacillus）、克雷伯氏菌属（klebsiella）、假单胞菌属（pseudomonas）、葡萄球菌属（staphylococcus）、肠杆菌属（enterobacter）.多样性分析表明，与青海高寒草地、雅安山区相比，成都平原产脂肪酶菌株遗传多样性更为丰富.在54株菌中都出现了300bp和1300bp两个特异且稳定出现的条带，这两个特异条带可能是产脂肪酶菌株的scar标记条带.
| [1] | janainanp,julianaabc,gláuciamp.characterizationofalkalinelipasefromfusariumoxysporumandtheeffectofdifferentsurfactantsanddetergentsontheenzymeactivity.brazjmicrobiol,2006,37:505~509
|
| [2] | burkertjf,maugerif,rodriguesmi.optimizationofextracellularlipaseproductionbygeotrichumsp.usingfactorialdesign.bioresourtechnol,2004,91(1):77~84
|
| [3] | guptar,guptan,rathip.bacteriallipases:anoverviewofproductionpurificationandbiochemicalproperties.applmicrobiol.biotechnol,2004,64(6):763~781
|
| [4] | daviesme.astudyofthediffusiblelipaseproducedbystaphylococciandofitsimmunologicalactivity.jgenmicrobiol,1954,11(1):37~44
|
| [5] | zhaolp(赵立平),xiaoh(肖红),liqy(李琴艳),zhangf(张峰).eric-pcrasanewtoolforquickidentificationofenvironmentalbacteria.chinjapplenvironbiol(应用与环境生物学报),1999,5(suppl):30~33
|
| [6] | chengdr,sunhc,xujs,gaos.pcrdetectionofvirulencefactorgenesinescherichiacoliisolatesfromweanedpigletswithedemadiseaseand/ordiarrheainchina.vetmicrobiol,2006,115(4):320~328
|
| [7] | sorokindy,jonesbe.improvedmethodfordirectscreeningoftruelipase-producingmicroorganismswithparticularemphasisonalkalineconditions.mikrobiologiia,2009,78(1):144~149
|
| [8] | ghosha,maityb,chakabartik,chattopadhyayd.bacterialdiversityofeastcalcuttawetlandarea:possibleidentificationofpotentialbacterialpopulationfordifferentbiotechnologicaluses.microbecol,2007,54(3):452~459
|
| [9] | ertugruls,donmezg,takaçs.isolationoflipaseproducingbacillussp.fromolivemillwastewaterandimprovingitsenzymeactivity.jhazardmater,2007,149(3):720~724
|
| [10] | zhangxm(张晓梅),douwf(窦文芳),xuhy(许泓瑜),xuzh(许正宏).identificationofalipaseproducinghalophilicstrainanditshalophilicmechanism.chinjapplenvironbiol(应用与环境生物学报),2010,16(1):100~103
|
| [11] | burrmd,josephsonkl,pepperil.anevaluationoferic-pcrandapfingerprintingfordiscriminatingsalmonellaserotypes.lettapplmicrobiol,1998,27(1):24~30
|
| [12] | klibanovam.improvingenzymesbyusingtheminorganicsolvents.nature,2001,409:241~246
|
| [13] | macedoga,parkyk,pastoregm.partialpurificationandcharacterizationofanextracellularlipasefromanewlyisolatedstrainofgeotrichumsp.brazjmicrobiol,1997,39:687~692
|
| [14] | ciafardinig,zulloba,iridea.lipaseproductionbyyeastsfromextravirginoliveoil.foodmicrobiol,2006,23(1):60~67
|
| [15] | yuanw,chaitj,miaozm.eric-pcridentificationofthespreadofairborneescherichiacoliinpighouses.scitotalenviron,2000,408(6):1446~1450
|
| [16] | rohlffj.ntsys-pcnumericaltaxonomyandmulti-variateanalysissystem.version2.1.exetersoftware,setauket,newyork,2000
|
| [17] | macraea.theuseof16srdnamethodsinsoilmicrobialecology.brazjmicrobiol,2000,31(2):77~82
|
| [18] | itos,kobayashit,arak,ozakik,kawais,hataday.alkalinedetergentenzymesfromalkaliphiles:enzymaticpropertiesgeneticsandstructures.extremophiles,1998,2(3):185~190
|
| [19] | josephb,ramtekepw,thomasg.coldactivemicrobiallipases:somehotissuesandrecentdevelopments.biotechnoladv,2008,26(5):457~470
|
| [20] | kulkarnin,gadrerv.productionandpropertiesofalkalinethermophiliclipasefrompseudomonasfluorescensns2w.jindmicrobiolbiotechnol,2002,28(6):344~348
|
| [21] | leclercje,lib,paynewl,cebulata.highmutationfrequenciesamongescherichiacolisalmonellapathogens.science,1996,274(5290):1208~1211
|
| [22] | potumarthir,subhakarc,vanajakshij,jettya.effectofaerationandagitationregimesonlipaseproductionbynewlyisolatedrhodotorulamucilaginosa-mtcc8737instirredtankreactorusingmolassesassolecarbonsource.applbiochembiotechnol,2008,151(2~3):700~710
|
| [23] | rodriguez-barradasmc,hamillrj,houstoned,georghioupr,clarridgeje,regneryrl,koehlerje.genomicfingerprintingofbartonellaspeciesbyrepetitiveelementpcrfordistinguishingspeciesandisolates.jclinmicrobiol,1995,33(5):1089~1093
|
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