|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
生物样品体外致突变高通量检测方法研究进展
DOI: 10.3724/SP.J.1145.2012.00518, PP. 518-523
Keywords: 致突变性,生物样品,高通量检测,ames实验,sos反应,单细胞凝胶电泳,tk基因,染色体损伤
Abstract:
评述了几种常用的体外致突变检测方法及其用于生物样品检测的可行性，有些方法经改进可用于高通量检测生物样品体外致突变性.经典ames实验受生物样品中组氨酸的影响,易产生假阳性结果，尽管经过修正可以排除组氨酸的干扰，但操作繁琐，不适合高通量检测.基于sos反应的检测体系避开了组氨酸的影响，且简单易行，适合高通量检测：以β-半乳糖苷酶基因（lacz）作为报告基因的检测体系灵敏度高，且经过离心洗涤或后培养的方式可降低样品颜色的影响；以绿色荧光蛋白（gfp）基因作为报告基因的检测体系避开了颜色的干扰，但这类方法灵敏度普遍不高，可以寻找信号更强的荧光蛋白以替代gfp；荧光素酶（lux）基因集lacz和gfp的优点于一身，但检测时需要额外添加辅助因子，限制了其应用.也对单细胞凝胶电泳、tk基因突变实验、染色体损伤检测等方法进行了分析，有些适合生物样品高通量检测，但由于缺少国际通用的标准，很难推广使用.
| [1] | 1amesbn,mccannj,yamasakie.methodsfordetectingcarcinogensandmutagenswiththesalmonella/mammalian-microsomemutagenicitytest.mutatres,1975,31:347~364
|
| [2] | 7fluckiger-islers,baumeisterm,braunk,gervaisv,hasler-nguyenn,reimannr,vangompelj,wunderlichhg,engelhardtg.assessmentoftheperformanceoftheamesiiassay:acollaborativestudywith19codedcompounds.gentoxicol&environmutagenesis,2004,558:181~197
|
| [3] | 8czyza,jasieckij,bogdana,szpilewskah,wegrzyng.geneticallymodifiedvibrioharveyistrainsaspotentialbioindicatorsofmutagenicpollutionofmarineenvironments.applenvironmicrobiol,2000,66:599~605
|
| [4] | 9podgrskab,chece,ulanowskak,wegrzyng.optomisationofthemicrobiologicalmutagenicityassaybasedongeneticallymodifiedvibrioharveyistrains.applgenet,2005,46:241~246
|
| [5] | 10grttera,griederichu,wurglerfe.themutagenicityofediblemushroomsinahistidine-independentbacterialtestsystem.foodchemtoxicol,1991,29:159~165
|
| [6] | 13mohtashamipure,norpothk,fiederl.isolationofframeshiftmutagensfromsmokers’urine:experienceswiththreeconcentrationmethods.carcinogenesis,1985,6(5):783~788
|
| [7] | 15yamasakie，amesbn.concentrationofmutagensfromurinebyadsorptionwiththenonpolarresinxad-2:cigarettesmokershavemutagenicurine.procnatlacadsciusa,1977,74(8):3555~3559
|
| [8] | 19liub,jinjl,chengyf,zhanghq,gaopj.amodifiedsuspensiontestforestimatingthemutagenicityofsamplescontainingfreeand(or)protein-boundhistidine.canjmicrobiol,2009,55:1~8
|
| [9] | 25quillardetp,huismano,arird,hofnungm.soschromotest,adirectassayofinductionofansosfunctioninescherichiacolik-12tomeasuregenotoxicity.procnatlacadsciusa,1982,79(19):5971~5975
|
| [10] | 26nunoshibat,nishiokah.‘rec-lactest’fordetectingsos-inducingactivityofenvironmentalgenotoxicsubstances.mutatres,1991,254:71~77
|
| [11] | 27oday,nakamuras,okii,katot,shinagawah.evaluationofthenewsystem(umu-test)forthedetectionofenvironmentalmutagensandcarcinogens.mutatres,1985,147:219~229
|
| [12] | 28nakamuras,oday,ugawam.inductionofumugeneexpressioninsalmonellatyphimuriumta1535/psk1002bydimethylsulfoxide(dmso).mutatres,1990,229:11~15
|
| [13] | 37oday,funasakak,kitanom,nakamaa,yoshikurat.useofahigh-throughputumu-microplatetestsystemforrapiddetectionofgenotoxicityproducedbymutageniccarcinogensandairborneparticulatematter.environmolmutagen,2004,43:10~19
|
| [14] | 38funasakak,kitanom,nakamaa,yoshikurat,oday.detectionofgenotoxicityofatmosphericparticlesusingahigh-throughputmicroplateumu-testsystem.actabiochimpol,2003,50(1):291~296
|
| [15] | 39oday,yamazakih,watanabem,nohmit,shimadat.developmentofhighsensitiveumutestsystem:rapiddetectionofgenotoxicityofpromutagenicaromaticaminesbysalmonellatyphimuriumstrainnm2009possessinghigho-acetyltransferaseactivity.mutatres,1995,334:145~156
|
| [16] | 40oday,yamazakih,watanabem,nohmit,shimadat,shimadat.highlysensitiveumutestsystemforthedetectionofmutagenicnirtoarenesinsalmonellatyphimuriumnm3009havingo-acetyltransferaseandnitroreductaseactivities.environmolmutagen,1993,21(4):357~364
|
| [17] | 41ozturkk,durusoym.thedetectionandcomparisonofthegenotoxiceffectsofsomenitroaromaticcompoundsbytheumuandsoschromotestsystems.toxicollett,1999,108:63~68
|
| [18] | 47justust,thomassm.evaluationoftranscriptionalfusionswithgreenfluorescentproteinversusluciferaseasreportersinbacterialmutagenicitytests.mutagenesis,1999,14(4):351~356
|
| [19] | 48sorensensj,burmollem,hansenlh.makingbio-senseoftoxicity:newdevelopmentsinwhole-cellbiosensors.curropinbiotechnol,2006,17:11~16
|
| [20] | 49sagie,hevern,rosenr,bartolomeaj,premkumarjr,ulberr,levo,schepert,belkins.fluorescenceandbioluminescencereporterfunctionsingeneticallymodifiedbacterialsensorstrains.sensactuatb,2003,90:2~8
|
| [21] | 53rojanapow,nagaom,kawachit,sugimurat.prophageλinductiontest(inductest)ofanti-tumorantibiotics.genettoxicol,1981,88(4):325~335
|
| [22] | 2jinjl(金建玲),liub(刘波),gaopj(高培基).thinkingofrevisingamessystemsuitedtoinvitromutagenicitytestforchinesemateriamedica.chintrad&herbaldrugs(中草药),2009,40(7):1155~1157
|
| [23] | 3nylundl,einistop.mutagenicitytestingofprotein-containingandbiologicalsamplesusingtheamse/salmonellaplateincorporationtestandthefluctuationtest.mutatres,1993,272:205~214
|
| [24] | 4koyaman,yasuim,oday,suzukis,satoht,suzukit,matsudat,masudas,kinaen,honmam.genotoxicityofacrylamideinvitro:acrylamideisnotmetabolicallyactivatedinstandardinvitrosystems.environmolmutagen,2011,52(1):9~11
|
| [25] | 5liub(刘波),jinjl(金建玲),zhangh(张辉),gaopj(高培基).uncertaintyofamestest.chinjapplenvironbiol(应用与环境生物学报),2007,13(5):726~730
|
| [26] | 6marondm,amesbn.revisedmethodsforthesalmonellamutagenicitytest.mutatres,1983,113:173~215
|
| [27] | 11schmidc,arndtc,reifferscheidg.mutagenicitytestsystembasedonareportergeneassayforshort-termdetectionofmutagens(mutagenassay).mutatres,2003,535:55~72
|
| [28] | 12cariellonf,narayanans,kwanyuenp,muthh,caseywm.anovelbacterialreversionandforwardmutationassaybasedongreenfluorescentprotein.mutatres,1998,414:95~105
|
| [29] | 14yamamotoh,mizutanit,nomurah.studiesonthemutagenicityofcrudedrugextracts.yakugakuzasshi,1982,102(6):596~601
|
| [30] | 16jinjl(金建玲),zhangh(张辉),liub(刘波),tianx(田晓),gaopj(高培基).射干水煎液的致突变性检验及ames测验的改进.actachinmedpharmacol(中医药学报),2009,37(3):12~16
|
| [31] | 17jinjl(金建玲),zhangh(张辉),liub(刘波),caiyp(蔡玉品),gaopj(高培基).mutagenicityofenphorbialunulatadecoctioninvitro.chinpharmacolbull(中国药理学通报),2010,26(2):263~266
|
| [32] | 18jinjl,liub,zhangh,tianx,caiypandgaopj.mutagenicityofchinesetraditionalmedicinesemenarmeniacaeamarumbytwomodifiedamestests.bmccomplement&alternativemed,2009,9:43~50
|
| [33] | 20burckhardtse,woodgater,scheuermannrh,echolsh.umudmutagenesisproteinofescherichiacoli:overproduction,purification,andcleavagebyreca.procnatlacadsciusa,1988,85:1811~1815
|
| [34] | 21humayunmz.sosandmayday:multipleinduciblemutagenicpathwaysinescherichiacoli.molmicrobiol,1998,30(5):905~910
|
| [35] | 22nohmit,hakuraa,nakaiy,watanabem,murayamasy,sofunit.salmonellatyphimuriumhastwohomologousbutdifferentumudcoperons:cloningofanewumudc-likeoperon(samab)presentina60-megadaltoncrypticplasmidofs.typhimurium.jbacteriol,1991,173(3):1051~1063
|
| [36] | 23janionc.induciblesosresponsesystemofdnarepairandmutagenesisinescherichiacoli.intjbiolsci,2008,4(6):338~344
|
| [37] | 24smithbt,walkergc.mutagenesisandmore:umudcandtheescherichiacolisosresponse.gensocam,1998,148:1599~1610
|
| [38] | 29kosakah,oday,uozumim.inductionofumucgeneexpressionbynitrogendioxideinsalmonellatyphimurium.mutatres,1985,142:99~102
|
| [39] | 30jinzc,qianj.plasmidpsk1002-mediatedmutatoreffectandsosresponseandsosmutagenesisof2,5-dichloronitrobenzolinsalmonellatyphimurium.mutatres,1991,264:1~5
|
| [40] | 31ongt,stewartj,wenyf,whongwz.applicationofsosumu-testforthedetectionofgenotoxicvolatilechemicalsandairpollutants.environmutagen,1987,9:171~176
|
| [41] | 32hamerb,biharin,reifferscheidg,zahnrk,mullerweg,batelr.evaluationofthesos/umu-testpost-treatmentassayforthedetectionofgenotoxicactivitiesofpurecompoundsandcomplexenvironmentalmixtures.mutatres,2000,466:161~171
|
| [42] | 33palak,rahmanms,chatterjecsn.ontheinductionofumugeneexpressioninsalmonellatyphimuriumstrainta1535/psk1002bysomenitrofurans.mutatres,1992,280:67~71
|
| [43] | 34ohtj,kimig.thesosinductionofumu-laczfusiongenebyoxidativedamageisinfluencedbypolyaminesinescherichiacoli.cellbioltoxicol,1999,15:291~297
|
| [44] | 35motohashin,saitoy.inductionofsosresponseinsalmonellatyphimuriumta4107/psk1002byperoxynitrite-generatingagent,n-morpholinosydnonimine.mutatres,2002,502:11~18
|
| [45] | 36reifferscheidg,heilj,oday,zahnrk.amicroplateversionofthesos/umu-testforrapiddetectionofgenotoxinsandgenotoxicpotentialsofenvironmentalsamples.mutatres,1991,253:215~222
|
| [46] | 42vahlhh,karbel,westendorfj.genotoxicityassessmentofsuspendedparticulatematterintheelberiver:comparisonofslamonellamicrosometest,arabinoseresistancetest,andumu-test.mutatres,1997,394:81~93
|
| [47] | 43schmidc,reifferscheidg,zahnrk,bachmannm.increaseofsensitivityandvalidityofthesos/umu-testafterreplacementoftheβ-galactosidasereportergenewithluciferase.mutatres,1997,397:9~16
|
| [48] | 44justust,thomassm.constructionofaumuc’-luxabplasmidforthedetectionofmutagenicdnarepairvialuminescence.mutatres,1998,398:131~141
|
| [49] | 45kostrzynskam,leungkt,leeh,trevorsjt.greenfluorescentprotein-basedbiosensorfordetectingsos-inducingactivityofgenotoxiccompounds.jmicrobiolmethods,2002,48:43~51
|
| [50] | 46arair,makitay,oday,nagamunet.constructionofgreenfluorescentproteinreportergenesforgenotoxicitytest(sos/umu-test)andimprovementofmutagen-sensitivity.jbioscibioeng,2001,92(3):301~304
|
| [51] | 50normana,hansenlh,sorensensj.aflowcytometry-optimizedassayusingansos-greenfluorescentprotein(sos-gfp)whole-cellbiosensorforthedetectionofgenotoxinsincomplexenvironments.mutatres,2006,693:164~172
|
| [52] | 51dikkijm,chenxy,shuaijb,chenn,fangwh.attenuatedsalmonellatyphimuriumasacarrierforprokaryoticandeukaryoticexpressionvectors.jzhejianguniv(浙江大学学报),2006,32(3):237~244
|
| [53] | 52mambersw,brysonvandkatzse.evaluationoftheescherichiacolik12inductestfordetectionofpotentialchemical.mutatres,1984,130(3):141~151
|
| [54] | 54hatanakan,yamazakih,oday,guengerichfp,nakajimam,yokoit.metabolicactivationofcarcinogenic1-nitropyrenebyhumancytochromep4501b1insalmonellatyphimuriumstrainexpressingano-acetyltransferaseinsos/umuassay.mutatres,2001,497(1~2):223~233
|
| [55] | 55lichtenberg-frateh,schmittm,gellertg,ludwigj.ayeast-basedmethodforthedetectionofcytoandgenetoxicity.toxicolinvitro,2003,17(5~6):709~716
|
| [56] | 56reifferscheidg,heilj.validationofthesos/umutestusingtestresultsof486chemicalsandcomparisonwiththeamestestandcarcinogenicitydata.mutatres,1996,369:129~145
|
| [57] | 57ostlingo,johansonkj.microelectrophoreticstudyofradiation-induceddnadamageinindividualmammaliancells.biochembiophysrescommun,1984,123:291~298
|
| [58] | 58singhnp,mccoymt,ticerr.asimpletechniqueforquantitationoflowlevelofdnadamageinindividualcells.expcellsres,1988,175:184~191
|
| [59] | 59liberhl,thillywg.mutationassayatthethymidinekinaselocusindiploidhumanlymphoblasts.mutatres,1982,94(2):467~485
|
| [60] | 60liub(刘波),dengzp(邓中平).tk基因突变的分子机制研究.carcinogteratog&mutag(癌变�畸变�突变),2005,17(3):190~192
|
| [61] | 61xingwp(刑卫平),zhaohk(赵恒奎),jiangzr(姜宗荣).微核及微核试验在遗传毒理学中的应用.anhuijpreventmed(安徽预防医学杂志),2002,8(5):317~319
|
| [62] | 62zhengk(郑科),panjw(潘建伟),jiangzm(姜志明),zhumy(朱睦元).姐妹染色单体(sce)的检测原理及其分子机理.jcellbiol(细胞生物学杂志),2002,24(6):355~359
|
| [63] | 63shengp(沈光平),wangqn(王钦南),zhouzz(周祉祯).微核与染色体畸变的相关性.hereditas(遗传),1985,7(1):15~17
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
