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OALib Journal期刊
ISSN: 2333-9721
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禾谷孢囊线虫侵染对小麦根部防御酶的影响
DOI: 10.3724/SP.J.1145.2012.00359, PP. 359-363
Keywords: 禾谷孢囊线虫,脂氧合酶,苯丙氨酸转氨酶,茉莉酸甲酯,小麦
Abstract:
以抗禾谷孢囊线虫（heteroderaavenaewollenweber，cerealcystnematode，ccn）小麦品系‘e-10’和易感材料‘中国春’为材料，通过人工接种禾谷孢囊线虫二龄幼虫，并在接种线虫后测定根部防御酶苯丙氨酸转氨酶（pal）和脂氧合酶（lox）活力变化，研究抗感性材料对线虫侵染后防御酶的响应，以了解作物的抗虫机制.结果发现，‘e-10’和‘中国春’防御酶活力变化存在显著差异，‘e-10’在线虫侵染后6h，lox酶活增强，在24h达到最大值，酶活变化比‘中国春’迅速，暗示‘e-10’启动线虫防御反应更为有效直接.此外，发现小麦在接种线虫后会引起邻近未接种植株根部防御酶活力变化.‘e-10’在接种线虫后12h，其接触组未接种材料根部pal酶活增加0.9倍，lox酶活增加1.1倍，并且‘e-10’未接种植株的酶活变化比‘中国春’更迅速，幅度更剧烈.表明可能有某些气态或挥发性物质参与了小麦防御线虫侵染过程，并且这种现象在抗感材料间差异显著.使用茉莉酸甲酯处理‘e-10’和‘中国春’，小麦根部pal和lox酶活并未产生与接触接种线虫材料相一致的变化，表明该气态或挥发性物质并非茉莉酸甲酯.
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