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OALib Journal期刊
ISSN: 2333-9721
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酵母蛋白激酶sch9激活环磷酸化状态参与热胁迫应答调控
DOI: 10.3724/SP.J.1145.2012.00369, PP. 369-372
Keywords: sch9,热胁迫,磷酸化,pdk1位点,酵母
Abstract:
？在酵母（saccharomycescerevisiae）中，蛋白激酶sch9已经被证明与细胞大小、胁迫应答、自噬以及寿命的调控有关.通过不同基因型酵母在固体培养基上的热敏感性研究，发现sch9的激酶功能与热胁迫相关，进一步通过sch9特异磷酸化位点抗体的免疫印迹，检测了细胞内sch9关键的激活环t570位点（pdk1位点）在热胁迫条下的磷酸化状态，发现半乳糖诱导表达的sch9在热胁迫条件下t570位点去磷酸化，首次证明了sch9通过调控激酶活性参与细胞热胁迫应答.研究结果揭示了sch9在胁迫应答中的部分功能，并为生理条件下sch9调控功能的研究提供了生物化学证据.
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