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OALib Journal期刊
ISSN: 2333-9721
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sch9蛋白激酶调控酵母细胞全蛋白的泛素化
DOI: 10.3724/SP.J.1145.2012.00364, PP. 364-368
Keywords: 酵母,sch9,泛素化,蛋白酶体,自噬,热胁迫
Abstract:
为探讨蛋白激酶sch9是否影响细胞蛋白质稳态水平及其在热胁迫时的响应，以野生型、sch9基因缺失突变体、转sch9基因δsch9（sch9-3ha）酵母为材料，利用特异的泛素抗体，检测长期培养时全蛋白泛素化水平变化.结果表明，sch9缺失引起细胞全蛋白泛素化水平降低，转sch9基因可以使细胞全蛋白泛素化水平恢复.55℃热胁迫30min处理，突变体δsch9全蛋白泛素化水平降低，转sch9基因δsch9(sch9-3ha)全蛋白泛素化水平不变，回到正常生长温度时，蛋白泛素化水平也没有改变.外加蛋白酶体抑制剂mg132时，突变体δsch9蛋白泛素化水平不受影响，而转sch9基因△sch9(sch9-3ha)全蛋白泛素化水平显著增加，表明前者是自噬途径降解，而增加部分是通过蛋白酶体途径降解.蛋白激酶sch9调控细胞全蛋白泛素化水平，以及2个不同的降解途径.
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