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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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细菌dietziasp.dq12-45-1b代谢十六烷产物分析和检测方法优化
DOI: 10.3724/SP.J.1145.2013.00519, PP. 519-522
Keywords: 十六烷,生物降解,表面活性物质,迪茨氏菌,tlc检测
Abstract:
为研究菌株dietziasp.dq12-45-1b降解十六烷产生的活性代谢产物，优化了该菌株产生的表面活性物质的提取和检测方法，分析了该菌株代谢十六烷不同时间产生表面活性物质的情况.结果显示，乙酸乙酯萃取法从培养液中回收代谢产物的效率最高，正己烷:氨水:异丙醇（2:1:4，v:v:v）的展层体系可均匀分开十六烷降解培养液萃取物.菌株dq12-45-1b能够以十六烷为底物产生糖脂类表面活性物质，产量随培养时间延长逐渐增加.十六烷乳化层与培养液水相中表面活性物质含量比为4.16:1，经检测发现，水相萃取物中含有一种表面活性物质，而乳化层萃取物中含有两种表面活性物质.
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