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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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家蝇纤维素酶的检测及酶活性与其发育的关系
DOI: 10.3724/SP.J.1145.2013.00444, PP. 444-448
Keywords: 纤维素酶,β-葡萄糖苷酶,内切β-1,4-葡聚糖酶,外切β-1,4-葡聚糖酶,酶活性,发育,家蝇
Abstract:
为了解家蝇能否降解利用纤维素，采用dnsa法检测家蝇幼虫纤维素酶活性及酶学特性；并进一步研究纤维素酶活性与家蝇发育的关系.结果显示，在生长发育的不同时期，家蝇都具有滤纸酶活性，活性范围为0.85(±0.035)iumg-1至10.45(±0.050)iumg-1；家蝇纤维素酶的组成包含3种酶，即β-葡萄糖苷酶（β-glucosidase，bg）、内切β-1,4-葡聚糖酶（endo-β-1,4glucanase，eg）和外切β-1,4-葡聚糖酶（exo-β-1,4-glucanase，cbh）.在ph5.6的条件下，这3种酶的最适反应温度和时间分别为50℃和60min，在体外具有较强的热稳定性，50℃放置1h后仍可保持较强的酶活性.纤维素酶活性随着家蝇的发育逐渐增高（p<0.05），卵的酶活性最低,成虫的酶活性最高，在最适反应条件下，成虫bg、eg和cbh的活性分别为(7.96±0.065)iumg-1、(9.76±0.080)iumg-1和(10.86±0.091)iumg-1.这3种酶的活性在家蝇卵、幼虫、蛹和成虫4个发育阶段，均是cbh最高，bg最低.增加家蝇幼虫饲料中粗纤维的含量，3龄幼虫的纤维素酶活性明显提高（p<0.05），而平均体重和体长与对照组比无显著差异（p>0.05）.研究表明家蝇具有完整的降解利用植物纤维素的酶系，其纤维素酶活水平与家蝇的发育密切相关.
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