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OALib Journal期刊
ISSN: 2333-9721
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类球红细菌lhⅱβ-亚基/α-亚基融合蛋白吸收光谱分析
DOI: 10.3724/SP.J.1145.2013.00395, PP. 395-398
Keywords: 类球红细菌,融合表达,柔性序列,捕光色素蛋白复合体ⅱ,近红外吸收光谱,蛋白鉴定
Abstract:
类球红细菌（rb.sphaeroides）捕光色素蛋白复合体ⅱ（lhⅱ）由9对β-亚基和α-亚基组成，分别是pucb和puca基因编码的.为探究β-亚基和α-亚基融合后是否能够形成正常结构与功能的lhⅱ，在pucb和puca基因之间加入一段柔性序列（flexiblesequence，fs），构建pucb-fs-puca融合表达载体，导入双缺失突变体rb.sphaeroidescqu68菌株中，经iptg诱导，近红外700-900nm吸收光谱扫描细菌活体，亲和层析分离纯化蛋白并进行sds-page电泳鉴定.与对照（未融合的菌株）相比，融合菌株在800nm和850nm附近也出现了两个典型的特征吸收峰，但吸收峰值略微降低并蓝移，电泳结果说明融合蛋白在类球红细菌中获得成功表达.可见，两亚基融合后能够形成有活性的lhⅱ并进入到光合膜系统.
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