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OALib Journal期刊
ISSN: 2333-9721
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辣椒cawrky5启动子的分离及其调控元件分析
DOI: 10.3724/SP.J.1145.2013.00389, PP. 389-394
刘志钦, 杨晟, 蔡金森, 黄雪盈, 马小玲, 石兰平, 王博, 郭吟枫, 陈桂信, 牟少亮, 官德义, 何水林
Keywords: 辣椒,cawrky5启动子,病原菌应答,机械损伤应答,烟草叶片瞬间表达
Abstract:
采用genomewalking方法从辣椒基因组dna中分离获得辣椒cawrky5基因上游的启动子序列，命名为cawrky5p.为了分析cawrky5p对病原菌和机械损伤的应答以及可能的调控区域，将该基因5’端顺序4个不同缺失片段分别与gus报告基因相融合构成表达载体，在农杆菌介导的烟草叶片瞬间表达系统中表达.结果表明：启动子tata框为起始密码子atg上游-140bp至-136bp之间的tatataa，同时含有多种与逆境胁迫相关的顺式作用元件，如w-box、s-box、meja应答元件和abre类似元件等；cawrkyk5基因上游-936bp启动子可以应答青枯病菌、抗病信号分子以及机械损伤；cawrky5p应答青枯病菌和茉莉酸甲酯（meja）的核心元件可能位于启动子-886bp至-489bp之间，应答乙烯（et）核心调控区域位于-936bp和-886bp之间，而应答机械损伤的核心元件位于启动子-336bp以及atg之间.
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