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冰乙酸水解法提高女贞子提取物中红景天苷含量

DOI: 10.3724/SP.J.1145.2013.00723, PP. 723-726

Keywords: 酸水解法,女贞子,红景天苷,正交试验

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Abstract:

了提高女贞子提取物中红景天苷的含量,以红景天苷和酪醇的含量为考察指标,采用高效液相色谱法测定其含量,利用正交试验设计优选女贞子提取物中红景天苷的水解工艺,并考察料液比、水解时间和水解温度对提取物中红景天苷含量的影响.结果发现其中冰乙酸与提取液的料液比以及水解时间对提取物中红景天苷的含量起着主要作用;最佳工艺条件为:女贞子浓缩液与冰乙酸体积比为20?1,100℃下回流20h.水解后红景天苷的含量约是未经酸水解的3倍.研究表明冰乙酸水解法可显著提高女贞子提取物中红景天苷的含量;该方法操作简单,后续处理容易,水解原料无毒,可以满足大规模工业提取的需要.图1表3参15

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