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OALib Journal期刊
ISSN: 2333-9721
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高分子量多环芳烃降解过程中菌种间的相互作用
DOI: 10.3724/SP.J.1145.2013.00677, PP. 677-682
Keywords: 高分子量多环芳烃,解环菌属,海杆菌属,生物修复,协同效应
Abstract:
为研究海洋微生物间降解高分子量多环芳烃（highmolecularweight-polycyclicaromatichydrocarbons，简称hmw-pahs）过程中的相互作用，筛选具有协同效应的菌群，以1株可降解hmw-pahs的解环菌属细菌cycloclasticussp.py97m和4株其他属细菌分别构建二元菌群降解芘和荧蒽，通过gc-ms测定hmw-pahs降解率，并采用发光细菌法测定其降解前后的生物毒性.结果表明，由py97m和marinobacternanhaiticusd15-8wt组成的菌群（py97m+d15-8w）对初始质量浓度均为0.1g/l的单一碳源芘、荧蒽21d后的降解率分别为67.40%和62.79%，相对于纯培养py97m分别提高了20.30%和20.29%.另外，该菌群对初始质量浓度均为0.1g/l的芘和荧蒽组成的混合碳源14d后的降解率分别为71.05%和67.36%；生物毒性检测结果显示，hmw-pahs经该菌群降解后其急性毒性和遗传毒性相对于母体都有显著降低.解环菌与海杆菌在芘和荧蒽的降解过程中表现出明显的协同效应，表明该菌群具有应用到hmw-pahs污染海洋环境生物修复的潜力.图5表1参24
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