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OALib Journal期刊
ISSN: 2333-9721
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青香茅和五节芒内生固氮菌的分离与生理生化鉴定
DOI: 10.3724/SP.J.1145.2013.00643, PP. 643-649
Keywords: 乙炔还原法,全细胞蛋白电泳,is-pcr,内生固氮菌,固氮酶活性,微生物多样性,青香茅,五节芒
Abstract:
为研究青香茅（cymbopogoncaesius）和五节芒（miscanthusfloridulus）内生固氮菌的多样性，基于两种无氮培养基结合乙炔还原法从这两种牧草中分别分离到30株和36株菌.采用sds-page（dodecylsulfatesodiumsalt-polyacrylamidegelelectrophoresis）全细胞蛋白电泳和is-pcr（insertionsequence-basedpcr）指纹图谱将这66株内生固氮菌聚类为10个类群（i-x，每群3株以上，共61个聚群菌株）和3个未归类小组（共5个未聚群菌株）.从10个聚类群和3个未归类小组中各选1株菌作为代表进行16srdna序列分析，发现它们与洋葱伯克霍尔德菌（burkholderiacepacia）、阴沟肠杆菌（enterobactercloacae）、固氮植物菌（phytobacterdiazotrophicus）、阿氏肠杆菌（enterobacterasburiae）、织片草螺菌（herbaspirillumseropedicae）、阪崎克洛诺菌（cronobactersakazakii）、水稻肠杆菌（enterobacteroryzae）、肺炎克雷伯氏菌（klebsiellapneumoniae）、溶解肠杆菌（enterobacterdissolvens）、无乳固氮螺螺（azospirillumamazonense）、都柏林克罗诺杆菌（cronobacterdublinensis）等的相似性分别达97%以上，表明所分离内生固氮菌多样性高.进一步对它们进行生理生化测定，结果表明有些菌株具泌氨能力、产吲哚、解磷、解钾效果，在农业生产上具有一定的应用潜力.图2表3参38
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