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OALib Journal期刊
ISSN: 2333-9721
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与水稻褐飞虱中肠刷状缘膜囊泡（bbmv）相结合的活性短肽筛选
DOI: 10.3724/SP.J.1145.2013.00637, PP. 637-642
Keywords: 水稻褐飞虱,bbmv,噬菌体短肽文库,中肠,短肽
Abstract:
参照鳞翅目和双翅目昆虫中肠刷状缘膜囊泡（bbmv）的提取方法，成功提取了水稻褐飞虱中肠bbmv蛋白，利用噬菌体短肽文库展示技术，筛选到2个能够与水稻褐飞虱中肠bbmv结合的短肽p1z和p2z.以egfp蛋白为标签通过pulldown和westernblot方法对短肽p1z、p2z与褐飞虱中肠bbmv的结合活性进行了验证.结果表明，利用噬菌体短肽文库展示技术，以褐飞虱中肠bbmv作为底物，经过3轮淘洗筛选到的短肽蛋白与褐飞虱中肠bbmv上的潜在受体具有较强的结合能力.通过对短肽结构域的分析发现，短肽结构α两亲区与β两亲区的分布情况是影响短肽与褐飞虱中肠bbmv结合的一个潜在因素.本研究成功筛选并验证了能够与褐飞虱中肠bbmv结合的短肽.图5表1参35
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