|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
番茄slwd1基因的克隆及slwd1与ddb1的相互作用
DOI: 10.3724/SP.J.1145.2013.00623, PP. 623-629
Keywords: 番茄,slwd1基因,cdt2,cul4-ddb1e3泛素连接酶,酵母双杂交,免疫共沉淀
Abstract:
在真核生物中，cdt2作为一个wd40家族蛋白，是cul4-ddb1e3泛素连接酶复合体的重要组成部分.其主要功能是将cul4-ddb1e3泛素连接酶和靶向蛋白连接起来，从而实现靶蛋白的泛素化修饰.利用rt-pcr技术和3’race技术，本研究在番茄中克隆到一个与人类cdt2同源的基因，命名为slwd1.通过对番茄slwd1蛋白序列与其它模式生物的蛋白序列进行氨基酸保守结构域分析和同源性比对，发现slwd1具有2个非常保守的wd40家族的dwd结构域.酵母双杂交和免疫共沉淀实验都证明，slwd1与ddb1具有相互作用；实时定量rt-pcr分析证明，slwd1基因在各个组织中都表达，为组成型表达，但是，在生殖器官中的表达量要明显比其它的组织高.图7表1参33
| [1] | pickartcm.backtothefuturewithubiqutin[j].cell,2004,116(2):181-190
|
| [2] | petroskimd,deshaiesrj.functionandregulationofcullin-ringubiquitinligases[j].natrevmolcellbiol,2005,6(1):9-20
|
| [3] | lijm,jinj.crlubiquitinligasesanddnadamageresponse[j].frontoncol,2012,2:29
|
| [4] | schwechheimerc,villalobosli.cullin-containinge3ubiquitinligasesinplantdevelopment[j].curropinplantbiol,2004,7:677-686
|
| [5] | jacksons,xiongy.crl4s:thecul4-ringe3ubiquitinligases[j].trendsbiochemsci,2009,34:562-570
|
| [6] | chenx,zhangy,douglasl,uv-damageddna-bindingproteinsaretargetsofcul4a-mediatedubiquitinationanddegradation[j].jbiolchem,2006,276(51):48175-48182
|
| [7] | angerss,lit,yix,maccossmj,moonrt,zhengn.moleculararchitectureandassemblyoftheddb1-cul4aubiquitinligasemachinery[j].nature,443(7111):590-593
|
| [8] | heyj,mccallcm,huj,zengy,xiongy.ddb1functionsasalinkertorecruitreceptorwd40proteinstocul4-roc1ubiquitinligases[j].genesdev,2006,20(21):2949-2954
|
| [9] | biedermanns，hellmannh.wd40andcul4-basede3ligases:lubricatingallaspectsoflife[j].trendsplantsci,2011,16(1):38-46
|
| [10] | kamura,t,maenakak,kotoshibas,matsumotom,kohda,conawayrc,conawayjw,nakayamaki.vhl-boxandsocs-boxdomainsdeterminebindingspecificityforcul2-rbx1andcul5-rbx2modulesofubiquitinligases[j].genesdev,18:3055-3065
|
| [11] | liuc,poiteleam,watsona,yoshidash,shimodac,holmbergc,nielseno,carram.transactivationofschizosaccharomycespombecdt2+stimulatesapcu4-ddb1-csnubiquitinligase[j].emboj,2005,24:3940-3951
|
| [12] | leejh,terzaghiw,gusmarolig,charronjb,yoonhj,chenh,heyj,xiongy,dengxw.characterizationofarabidopsisandricedwdproteinsandtheirrolesassubstratereceptorsforcul4-ringe3ubiquitinligases[j].plantcell,2008,20(1):152-167
|
| [13] | higal,banksd,wum,kobayashir,sunh,zhangh.l2dtl/cdt2interactswiththecul4/ddb1complexandpcnaandregulatescdt1proteolysisinresponsetodnadamage[j].cellcycle,2006,5:1675-1680
|
| [14] | zhangy,fengs,chenf,chenh,wangj,mccallc,xiongy,dengxw.arabidopsisddb1-cul4associatedfactor1formsanucleare3ubiquitinligasewithddb1andcul4thatisinvolvedinmultipleplantdevelopmentalprocesses[j].plantcell,2008,20(6):1437-1455
|
| [15] | chenh,huangx,gusmarolig,terzaghiw,lauos,yanagaway,zhangy,lij,leejh,zhud,dengxw.arabidopsiscullin4-damageddnabindingprotein1interactswithconstitutivelyphotomorphogenic1-suppressorofphyacomplexestoregulatephotomorphogenesisandfloweringtime[j].plantcell,2010,22:108-123
|
| [16] | dumbliauskase,lechnere,jaciubekm,berra,pazhouhandehm,aliouam,cognatv,brukhinv,konczc,grossniklausu,molinierj,genschikp.thearabidopsiscul4�ddb1complexinteractswithmsi1andisrequiredtomaintainmedeaparentalimprinting[j].emboj,2011,30:731-743
|
| [17] | pazhouhandehm,molinierj,berra,genschikp.msi4/fveinteractswithcul4-ddb1andaprc2-likecomplextocontrolepigeneticregulationoffloweringtimeinarabidopsis[j].procnatlacadsciusa,2011,108:3430-3435
|
| [18] | leejh,yoonhj,terzaghiw,martinezc,daim,lij,byunmo,dengxw.dwa1anddwa2,twoarabidopsisdwdproteincomponentsofcul4-basede3ligases,acttogetherasnegativeregulatorsinabasignaltransduction[j].plantcell,2010,22:1716-1732
|
| [19] | wangs,liuj,fengy,niux,giovannonij,liuy.alteredplastidlevelsandpotentialforimprovedfruitnutrientcontentbydownregulationofthetomatoddb1-interactingproteincul4[j].plantj,2008,55:89-103
|
| [20] | liuy,roofs,yez,barryc,vantuinena,vrebalovj,bowlerc,giovannonij.manipulationoflightsignaltransductionasameansofmodifyingfruitnutritionalqualityintomato[j].procnatlacadsciusa,2004,101:9897-9902
|
| [21] | liuj,tangx,gaol,gaoy,liy,huangs,sunx,miaom,zengh,tianx,niux,zhengl,giovannonij,xiaof,liuy.aroleoftomatouv-damageddnabindingprotein1(ddb1)inorgansizecontrolviaanepigeneticmanner[j].plosone,2012,7:e42621
|
| [22] | roodbarkelarif,bramsiepej,weinlc,marquardts,novákb,jakobymj,lechnere,genschikp,schnittgera.cullin4-ringfinger-ligaseplaysakeyroleinthecontrolofendoreplicationcyclesinarabidopsistrichomes[j].procnatlacadsciusa,2010,107:15275-15280
|
| [23] | bennettej,rushj,gygisp,harperjw.dynamicsofcullin-ringubiquitinligasenetworkrevealedbysystematicquantitativeproteomics[j].cell,2010,143(6):951-965
|
| [24] | 刘继恺,高永峰,牛向丽,刘永胜.番茄hp1和hp2基因rna共干涉载体的构建及遗传转化[j].应用与环境生物学报,2009,15(5):591-595[liujk,gaoyf,niuxl,liuys.constructionandtransformationofco-rnaivectoroftomatohp1andhp2genes[j].chinjapplenvironbiol,2009,15(5):591-
|
| [25] | 李颖楠,冯媛媛,刘永胜.番茄cdt2同源基因rna干涉载体的构建及遗传转化研究[j].四川大学学报(自然科学版),2011,48(4):917-923[liyn,fengyy,liuys.constructionandtransformationofrnaivectoroftomatohomologuecdt2[j].jsichuanuniv(natscied),2011,48(4):917-
|
| [26] | liebermanm,segevo,gilboan,lalazara,levini.thetomatohomologofthegeneencodinguv-damageddnabindingprotein1(ddb1)underlinedasthegenethatcausesthehighpigment-1mutantphenotype[j].theorapplgenet,2004,108:1574-1581
|
| [27] | jin,j,ariasee,chenj,harperjw,walterjc.afamilyofdiversecul4-ddb1-interactingproteinsincludescdt2,whichisrequiredforsphasedestructionofthereplicationfactorcdt1[j].molcell,2004,23:709-721
|
| [28] | sengat,sivaprasadu,zhuw,parkjh,ariasee,walterjc,duttaa.pcnaisaco-factorforcdt1degradationbycul4/ddb1mediatedn-terminalubiquitination[j].jbiolchem,2006,281:6246-6252
|
| [29] | melixetianm,ballabenia,masierol,gasparinip,zamponir,bartekj,lukasj,helink.lossofgeminininducesrereplicationinthepresenceoffunctionalp53[j].jcellbiol,165:473-482
|
| [30] | nishitanih,shiomiy,iidah,michishitam,takamit,tsurimotot.cdkinhibitorp21isdegradedbyaproliferatingcellnuclearantigen-coupledcul4-ddb1cdt2pathwayduringsphaseandafteruvirradiation[j].jbiolchem,2008,283:29045-29052
|
| [31] | abbast,shibatae,parkj,jhas,karnanin,duttaa.crl4cdt2regulatescellproliferationandhistonegeneexpressionbytargetingpr-set7/set8fordegradation[j].molcell,2010,40:9-21
|
| [32] | odah,hübnermr,beckdb,vermeulenm,hurwitzj,spectordl,reinbergd.regulationofthehistoneh4monomethylasepr-set7bycrl4cdt2-mediatedpcna-dependentdegradationduringdnadamage[j].molcell,2010,12,40(3):364-376
|
| [33] | o’connellbc,harperjw.ubiquitinproteasomesystem(ups):whatcanchromatindoforyou[j]?curropincellbiol,2007,19(2):206-214
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
