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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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一株乙醇利用菌的分离及其在乙醇含量测定中的应用
DOI: 10.3724/SP.J.1145.2009.00559, PP. 559-562
Keywords: 乙醇,嗜有机甲基杆菌,测定,电化学
Abstract:
从山西清徐农用沼气池中分离得到一株能以乙醇为唯一碳源和能源生长的菌株me45，通过生理生化特征及16srdna序列分析，将me45鉴定为嗜有机甲基杆菌(methylobacteriumorganophilium).依据me45能在有氧条件下利用乙醇的特性，因此以固定化细胞和溶氧仪组成测定系统测定了不同含量乙醇的响应时间以及溶氧变化与乙醇含量的关系.结果表明，乙醇含量在0.02%~2%(v/v)范围内，响应时间小于25min，溶氧消耗量同乙醇含量呈函数关系，拟合系数(r2)为0.9947.干扰实验结果表明，该测定体系具有较强的选择性，待测乙醇样品中其它醇类物质含量不高于乙醇含量5倍对乙醇测定无干扰.以95%乙醇为样品进行多次测定，测定平均值为91.8%，rsd为3.5%，表明该反应体系重现性良好.本研究为进一步研究乙醇微生物传感器奠定了基础.图7表1参15
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